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Anti-SIRT1 Antibody (R3V82)

Catalog #:   RHJ38701 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: FCM, IF, IHC, WB
Accession: Q96EB6
Overview

Catalog No.

RHJ38701

Species reactivity

Human, Mouse, Rat, Rabbit, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

NAD-dependent protein deacylase sirtuin-1, hSIR2, NAD-dependent protein deacetylase sirtuin-1, SIR2-like protein 1, 75SirT1, SIR2L1, Regulatory protein SIR2 homolog 1, SIRT1, hSIRT1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q96EB6

Applications

FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3V82

Data Image
  • Flow Cytometry
    Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).
  • Immunofluorescence
    Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse testis(A)Mouse kidney(B) using SIRT1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat spleen(A)Rat liver(B) using SIRT1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat spleen(A)Rat liver(B) using SIRT1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit spleen(A)Rabbit liver(B) using SIRT1 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysate.
  • Western blot
    Western blot analysis using SIRT1 mouse mAb against L1210(1),F9,(2),Hela(3) cell lysate.
References

Impact of sirtuin‑1 expression on progression‑free survival in non‑endometrioid endometrial cancer: A retrospective cohort study., PMID:40486084

Resveratrol reduces the activation of NLRP3 inflammasomes in rheumatoid arthritis through SIRT1 and ITGB α5β1, especially in patients with high expression of ACPA., PMID:40480022

Buzhong Yiqi decoction improves inflammation and oxidative damage in autoimmune thyroiditis by inhibiting apoptosis via the SIRT1-Mediated Nrf2/NF-κB axis., PMID:40360040

Discovery of an ApoE4-targeted small-molecule SirT1 enhancer for the treatment of Alzheimer's disease., PMID:40269061

The Clinical Role of SIRT-3 in the Acute Rejection Process of Kidney Transplantation and Its Effects on Graft Outcomes: Evaluation of Biomarker Potential., PMID:40142268

CD44 in myeloid cells is a major driver of liver inflammation and injury in alcohol-associated liver disease., PMID:39808819

Metabolic-Immune Suppression Mediated by the SIRT1-CX3CL1 Axis Induces Functional Enhancement of Regulatory T Cells in Colorectal Carcinoma., PMID:39783838

Intraperitoneal Treatment of Cambinol, a Synthetic SIRT1 and SIRT2 Inhibitory Compound, Exacerbates Brucella abortus 544 Burden in the Spleens of Institute of Cancer Research Mice., PMID:39770737

The Prognostic Impact of SIRT1, STAT3, and YAP1 in Colorectal Carcinoma., PMID:39636316

Disruption of PCSK9 Suppresses Inflammation and Attenuates Abdominal Aortic Aneurysm Formation., PMID:39588646

CD38 modulates cytokine secretion by NK cells through the Sirt1/NF-κB pathway, suppressing immune surveillance in colorectal cancer., PMID:39562615

Bone-protective effects of neutralizing angiopoietin-like protein 4 monoclonal antibody in rheumatoid arthritis., PMID:39367607

7D, a small molecule inhibits dengue infection by increasing interferons and neutralizing-antibodies via CXCL4:CXCR3:p38:IRF3 and Sirt1:STAT3 axes respectively., PMID:39284947

Comparison of SNCG and NEFH Promoter-Driven Expression of Human SIRT1 Expression in a Mouse Model of Glaucoma., PMID:39177995

Based on SIRT1/NF-κB/NLRP3 Signal Pathway to Explore the Effect of Yiqi Huatan Huoxue Recipe on Inflammatory Injury in AIT Mice by Pyroptosis., PMID:39136510

Effect of Urolithin A on the Improvement of Circadian Rhythm Dysregulation in Intestinal Barrier Induced by Inflammation., PMID:39064706

Sleep deprivation induced fat accumulation in the visceral white adipose tissue by suppressing SIRT1/FOXO1/ATGL pathway activation., PMID:38856814

Two-year post-distraction cartilage-related structural improvement is accompanied by increased serum full-length SIRT1., PMID:38790038

Conserved role of hnRNPL in alternative splicing of epigenetic modifiers enables B cell activation., PMID:38744970

Inhibition of de novo ceramide synthesis by sirtuin-1 improves beta-cell function and glucose metabolism in type 2 diabetes., PMID:38739545

Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission., PMID:38697011

Engineering of VCAM-1-targeted nanostructured lipid carriers for delivery of melatonin against acute lung injury through SIRT1/NLRP3 mediated pyroptosis signaling pathway., PMID:38490396

Reciprocal regulation of SIRT1 and AMPK by Ginsenoside compound K impedes the conversion from plasma cells to mitigate for podocyte injury in MRL/lpr mice in a B cell-specific manner., PMID:38465215

D-galacto-D-mannan-mediated Dectin-2 activation orchestrates potent cellular and humoral immunity as a viral vaccine adjuvant., PMID:38433834

Inhibition of CD38 enzymatic activity enhances CAR-T cell immune-therapeutic efficacy by repressing glycolytic metabolism., PMID:38307031

Investigating Forkhead Box O Transcription Factor 1 Gene's Relation to Immunoglobulin E in House Dust Mite-Allergic Asthma Patients., PMID:37987301

The anti-inflammatory activity of probiotic Dadiah to activate Sirtuin-1 in inhibiting diabetic nephropathy progression., PMID:37975108

Senescence-Targeted and NAD+-Dependent SIRT1-Activated Nanoplatform to Counteract Stem Cell Senescence for Promoting Aged Bone Regeneration., PMID:37948437

Mechanisms of weight-loss effect in obese mice by the endogenous cannabinoid receptor 2 agonist beta-caryophyllene., PMID:37919194

Assessment of global histone acetylation in pediatric and adolescent obesity: Correlations with SIRT1 expression and metabolic-inflammatory profiles., PMID:37862340

Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells., PMID:37781569

A1BG-AS1 promotes the biological functions of osteosarcoma cells via regulating the microRNA-148a-3p/USP22 axis and stabilizing the expression of SIRT1 through deubiquitinase function., PMID:37747800

Crocin improves the renal autophagy in rat experimental membranous nephropathy via regulating the SIRT1/Nrf2/HO-1 signaling pathway., PMID:37724538

A new insight into GH regulation and its disturbance from nutrition and autoimmune perspectives., PMID:37544742

Epigenomics Analysis of the Suppression Role of SIRT1 via H3K9 Deacetylation in Preadipocyte Differentiation., PMID:37511041

SARS-CoV-2 infection dysregulates NAD metabolism., PMID:37457744

Chronic SIRT1 supplementation in diabetic mice improves endothelial function by suppressing oxidative stress., PMID:37401647

The Association between Blood SIRT1 and Ghrelin, Leptin, and Antibody Anti-Hypothalamus: A Comparison in Normal Weight and Anorexia Nervosa., PMID:37373917

High-Dosage NMN Promotes Ferroptosis to Suppress Lung Adenocarcinoma Growth through the NAM-Mediated SIRT1-AMPK-ACC Pathway., PMID:37173894

DR region of NKAα1 is a target to ameliorate hepatic lipid metabolism disturbance in obese mice., PMID:37127227

VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation., PMID:37114564

The cardioprotective role of sirtuins is mediated in part by regulating KATP channel surface expression., PMID:36878847

Serum sirtuin-1 a potential marker in the diagnosis of rheumatoid arthritis., PMID:36843438

Gypenosides regulate autophagy through Sirt1 pathway and the anti-inflammatory mechanism of mitochondrial autophagy in systemic lupus erythematosus., PMID:36700474

CCL3 secreted by hepatocytes promotes the metastasis of intrahepatic cholangiocarcinoma by VIRMA-mediated N6-methyladenosine (m6A) modification., PMID:36691046

Short-chain fatty acids ameliorate necrotizing enterocolitis-like intestinal injury through enhancing Notch1-mediated single immunoglobulin interleukin-1-related receptor, toll-interacting protein, and A20 induction., PMID:36410023

Protein succinylation associated with the progress of hepatocellular carcinoma., PMID:36308411

HDAC4 depletion ameliorates IL-13-triggered inflammatory response and mucus production in nasal epithelial cells via activation of SIRT1/NF-κB signaling., PMID:36301023

Recombinant Humanized IgG1 Antibody Protects against oxLDL-Induced Oxidative Stress and Apoptosis in Human Monocyte/Macrophage THP-1 Cells by Upregulation of MSRA via Sirt1-FOXO1 Axis., PMID:36233020

Saponins from Panax notoginseng ameliorate steroid resistance in lupus nephritis through regulating lymphocyte-derived exosomes in mice., PMID:36210823

Datasheet

Document Download

Anti-SIRT1 Antibody (R3V82).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-SIRT1 Antibody (R3V82) [RHJ38701]
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