Anti-MLKL Antibody (R3V41)

Catalog No.

RHJ23605

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:50-1:100

Target

Mixed lineage kinase domain-like protein, MLKL, hMLKL

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q8NB16

Applications

IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3V41

Ficolin-3 Activates Complement and Triggers Necroptosis in Cholangiocarcinoma Cells via the RIPK1/RIPK3/MLKL Signaling Pathway., PMID:40407231

TNF-α antagonist alleviates muscular layer enlargement but does not prevent myenteric neuronal loss in the colon of streptozotocin-induced diabetic rats., PMID:40288461

MLKL-Mediated Necroptosis Predominantly Contributes to Immune-Associated Myocardial Damage., PMID:40195182

A determination of the main regulators of necroptosis in testicular tissue under different heat stresses., PMID:39856359

Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells., PMID:39633178

Serum HMGB-1 released by ferroptosis and necroptosis as a novel potential biomarker for systemic lupus erythematosus., PMID:39128419

Mechanisms of programmed cell death associated to severe dengue in human renal lesions., PMID:39025381

Metabolic changes enhance necroptosis of type 2 diabetes mellitus mice infected with Mycobacterium tuberculosis., PMID:38728367

PANoptosis Features, a Humanized NSG Murine Model of Sjogren's Syndrome., PMID:38635961

Pharmacological inhibition of ICOS attenuates the protective effect of exercise on cardiac fibrosis induced by isoproterenol., PMID:38224847

The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells., PMID:37995810

Activation of the TNF-α-Necroptosis Pathway in Parvalbumin-Expressing Interneurons of the Anterior Cingulate Cortex Contributes to Neuropathic Pain., PMID:37895135

Vagus Nerve Stimulation Prevents Endothelial Necroptosis to Alleviate Blood-Spinal Cord Barrier Disruption After Spinal Cord Injury., PMID:37460917

Major adverse cardiovascular events are associated with necroptosis during severe COVID-19., PMID:37081485

SARS-CoV-2 immune complex triggers human monocyte necroptosis., PMID:36870284

Necroptosis Drives Major Adverse Cardiovascular Events During Severe COVID-19., PMID:36711834

Cannabinoid Receptor 2-Centric Molecular Feedback Loop Drives Necroptosis in Diabetic Heart Injuries., PMID:36448459

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells., PMID:36342164

Necroptosis Plays a Crucial Role in Vascular Injury during DVT and Is Enhanced by IL-17B., PMID:36046722

The HSP90 inhibitor 17-DMAG alleviates primary biliary cholangitis via cholangiocyte necroptosis prevention., PMID:36037317

Preparation of the RIPK3 Polyclonal Antibody and Its Application in Immunoassays of Nephropathogenic Infectious Bronchitis Virus-Infected Chickens., PMID:36016369

Diverse actions of sirtuin-1 on ovulatory genes and cell death pathways in human granulosa cells., PMID:35840944

An IgM monoclonal antibody against domain 1 of CD147 induces non-canonical RIPK-independent necroptosis in a cell type specific manner in hepatocellular carcinoma cells., PMID:35598753

Necroptotic TNFα-Syndecan 4-TNFα Vicious Cycle as a Therapeutic Target for Preventing Temporomandibular Joint Osteoarthritis., PMID:35278225

Kupffer cells play a crucial role in monocrotaline-induced liver injury by producing TNF-α., PMID:35065160

TNF-α-dependent neuronal necroptosis regulated in Alzheimer's disease by coordination of RIPK1-p62 complex with autophagic UVRAG., PMID:34646380

Alendronate Augments Lipid A-Induced IL-1α Release via Activation of ASC but Not Caspase-11., PMID:34080091

Airway epithelial cell necroptosis contributes to asthma exacerbation in a mouse model of house dust mite-induced allergic inflammation., PMID:34045680

Boosting mitochondria activity by silencing MCJ overcomes cholestasis-induced liver injury., PMID:33997750

Cell death of hippocampal CA1 astrocytes during early epileptogenesis., PMID:33955001

MLKL inhibits intestinal tumorigenesis by suppressing STAT3 signaling pathway., PMID:33767595

A toolbox for imaging RIPK1, RIPK3, and MLKL in mouse and human cells., PMID:33589776

The necroptotic cell death pathway operates in megakaryocytes, but not in platelet synthesis., PMID:33510145

Detection of necroptosis by phospho-MLKL immunohistochemical labeling., PMID:33458710

Inducing regulated necrosis and shifting macrophage polarization with anti-EMMPRIN antibody (161-pAb) and complement factors., PMID:33205451

Increased MLKL mRNA level in the PBMCs is correlated with autoantibody production, renal involvement, and SLE disease activity., PMID:33054864

Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production., PMID:32595496

Protein-Bound Polysaccharides from Coriolus Versicolor Induce RIPK1/RIPK3/MLKL-Mediated Necroptosis in ER-Positive Breast Cancer and Amelanotic Melanoma Cells., PMID:32531147

Squalene emulsion-based vaccine adjuvants stimulate CD8 T cell, but not antibody responses, through a RIPK3-dependent pathway., PMID:32515732

Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis., PMID:32428502

Endoplasmic reticulum stress occurs in association with the extrusion of toxic extracellular vesicles from human placentae treated with antiphospholipid antibodies., PMID:32068238

Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages., PMID:32051018

HMGB1-associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats., PMID:31961020

Necroptotic cell binding of β2 -glycoprotein I provides a potential autoantigenic stimulus in systemic lupus erythematosus., PMID:31187539

Pathogenesis of lupus nephritis: RIP3 dependent necroptosis and NLRP3 inflammasome activation., PMID:31133359

FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation., PMID:31028177

Complement C5b-9 and Cancer: Mechanisms of Cell Damage, Cancer Counteractions, and Approaches for Intervention., PMID:31024572

Necroptotic Cell Death Promotes Adaptive Immunity Against Colonizing Pneumococci., PMID:31019504

RIP1 kinase inhibitor halts the progression of an immune-induced demyelination disease at the stage of monocyte elevation., PMID:30837313

The pathological features of regulated necrosis., PMID:30714148

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