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Anti-MIB1 Antibody (R3V29)

Catalog #:   RHJ17603 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, WB
Accession: Q86YT6
Overview

Catalog No.

RHJ17603

Species reactivity

Human, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP-1, Mind bomb homolog 1, ZZANK2, DAPK-interacting protein 1, E3 ubiquitin-protein ligase MIB1, KIAA1323, DIP1, RING-type E3 ubiquitin transferase MIB1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q86YT6

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3V29

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using MIB1 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using MIB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Western blot
    Western blot analysis using MIB1 mouse mAb against Hela (1) and COS7 (2) cell lysate.
References

Analytical Comparison of Commonly Used Laboratory-Developed Tests for the Assessment of Ki-67 in Breast Carcinoma With an FDA-Approved Benchmark., PMID:40516608

Congress of Neurological Surgeons systematic review and evidence based guideline on neuropathology for WHO grade II diffuse glioma: update., PMID:39747718

P53 and Ki67 Biomarkers are Predictors for Malignant Transformation in Oral Submucous Fibrosis: A Prospective Study., PMID:39733401

The combined assessment of p16INK4a and Mib/Ki-67 in oral squamous cell carcinoma., PMID:39664175

Comparison of MIB-1-Specific Membrane Staining in Hyalinising Trabecular Tumor Using Mainstream Automated Immunohistochemical Staining Platforms., PMID:39447085

Sjögren syndrome induced by anti PDL-1 treatment for TNBC: case report and review of literature., PMID:39434886

Flow cytometric analysis for Ki67 assessment in formalin-fixed paraffin-embedded breast cancer tissue., PMID:39183273

Immunohistochemical Profile of p62/SQSTM1/Sequestosome-1 in Human Low- and High-Grade Intracranial Meningiomas., PMID:39131899

POTENTIAL DIAGNOSTIC BIOMARKERS FOR HUMAN MESENCHYMAL TUMORS, ESPECIALLY LMP2/Β1I AND CYCLIN E1/MIB1 DIFFERENTIAL EXPRESSION: PRUM-IBIO STUDY., PMID:39089269

Meningioma Prognostic Tool Based on Correlation of Histopathological Grading and Immunohistochemistry., PMID:39055905

The expression of c-MYC, Cyclin D1 and Ki-67/MIB-1 in benign and malignant thyroid tissues: is there any diagnostic value?, PMID:39020541

[Plasmablastic lymphoma presenting with plasmacytosis and polyclonal hypergammopathy]., PMID:38448005

Development of a flow cytometric panel to assess prognostic biomarkers in fine needle aspirates of canine cutaneous or subcutaneous mast cell tumors., PMID:38076567

Voxel-based clustered imaging by multiparameter diffusion tensor images for predicting the grade and proliferative activity of meningioma., PMID:37644780

Comparison Between Manual and Automated Assessment of Ki-67 in Breast Carcinoma: Test of a Simple Method in Daily Practice., PMID:37559526

The Approach of Artificial Intelligence in Neuroendocrine Carcinomas of the Breast: A Next Step towards Precision Pathology?-A Case Report and Review of the Literature., PMID:37109630

Multiparametric magnetic resonance imaging for radiation therapy response monitoring in soft tissue sarcomas: a histology and MRI co-registration algorithm., PMID:37056570

Ki-67, topoisomerase IIα and miR-221 have a limited prostate cancer risk stratification ability on a medium-term follow-up: results of a high-risk radical prostatectomy cohort., PMID:36217395

Expression of Ki67 as detected by MIB-1 and its association with histopathological high-risk factors among patients with retinoblastoma tumour: a cross-sectional study., PMID:36161862

Systematically higher Ki67 scores on core biopsy samples compared to corresponding resection specimen in breast cancer: a multi-operator and multi-institutional study., PMID:35729220

Desmoplastic Small Round Cell Tumor of the Uterus: A Report of Molecularly Confirmed Case with EWSR1-WT1 Fusion., PMID:35626339

Spatial Metabolomics Identifies Distinct Tumor-Specific Subtypes in Gastric Cancer Patients., PMID:35395077

Establishment and Characterization of Brain Cancer Primary Cell Cultures From Patients to Enable Phenotypic Screening for New Drugs., PMID:35370679

Lymphoid hyperplasia with a polyp form of the gallbladder macroscopically mimicking carcinoma., PMID:35091990

Ketogenic Metabolic Therapy, Without Chemo or Radiation, for the Long-Term Management of IDH1-Mutant Glioblastoma: An 80-Month Follow-Up Case Report., PMID:34136522

Expression patterns for Bcl-2, EMA, β-catenin, E-cadherin, PAX8, and MIB1 in thymomas., PMID:34135467

A simple digital image analysis system for automated Ki67 assessment in primary breast cancer., PMID:33590538

UK NEQAS ICC & ISH Ki-67 Data Reveal Differences in Performance of Primary Antibody Clones., PMID:33337635

A juvenile case of epilepsy-associated, isocitrate dehydrogenase wild-type/histone 3 wild-type diffuse glioma with a rare BRAF A598T mutation., PMID:32996219

Myoepithelial Tumors of Salivary Gland: A Clinicopathologic and Immunohistochemical Study of 15 Patients with MIB-1 Correlation., PMID:32959208

A method to obtain reproducible Ki-67 indices in lung adenocarcinoma., PMID:32813926

Prediction of relapse in stage I testicular germ cell tumor patients on surveillance: investigation of biomarkers., PMID:32758242

Comparison of different anti-Ki67 antibody clones and hot-spot sizes for assessing proliferative index and grading in pancreatic neuroendocrine tumours using manual and image analysis., PMID:32617996

PSMD9 expression correlates with recurrence after radiotherapy in patients with cervical cancer., PMID:32565983

Preoperative denosumab treatment with curettage may be a risk factor for recurrence of giant cell tumor of bone., PMID:32539628

Performance analysis of the anti-Ki67 antibody clone 30-9 for immunohistochemical staining of breast cancer., PMID:32440959

Spontaneous pancreatic undifferentiated pleomorphic sarcoma in a laboratory rat: A case report., PMID:31773099

Correlation Between Digital and Manual Determinations of Ki-67/MIB-1 Proliferative Indices in Human Meningiomas., PMID:31771372

Impact of Primary Antibody Clone, Format, and Stainer Platform on Ki67 Proliferation Indices in Breast Carcinomas., PMID:31460869

CytoLyt fixation significantly inhibits MIB1 immunoreactivity whereas alternative Ki-67 clone 30-9 is not susceptible to the inhibition: Critical diagnostic implications., PMID:31398281

A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa., PMID:31281635

18F-Fluoroethyl-tyrosine uptake is correlated with amino acid transport and neovascularization in treatment-naive glioblastomas., PMID:31289907

Two ways to inactivate the Ki-67 protein-Fragmentation by nanoparticles, crosslinking with fluorescent dyes., PMID:31251462

Mindbomb Homolog-1 Index in the Prognosis of High-Grade Glioma and Its Clinicopathological Correlation., PMID:31001003

Subtypes of Breast Cancer in Lao P.D.R.: A Study in a Limited-Resource Setting., PMID:30806064

Angiogenic regeneration defines loco-regional recurrence following pre-operative radio-chemotherapy for rectal cancer: a pilot study., PMID:30721420

SMARCB1- and vimentin-positive esophageal carcinoma with undifferentiated components, rhabdoid features, and a good prognosis: a case report., PMID:30649642

LC3A, LC3B and Beclin-1 Expression in Gastric Cancer., PMID:30504397

Ki67 reproducibility using digital image analysis: an inter-platform and inter-operator study., PMID:30181553

The Combination Of Weak Expression Of PRDX4 And Very High MIB-1 Labelling Index Independently Predicts Shorter Disease-free Survival In Stage I Lung Adenocarcinoma., PMID:30013444

Datasheet

Document Download

Anti-MIB1 Antibody (R3V29).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-MIB1 Antibody (R3V29) [RHJ17603]
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