Catalog No.
RHH21702
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
NEDD-8, NEDD8, Neural precursor cell expressed developmentally down-regulated protein 8, Neddylin, Ubiquitin-like protein Nedd8
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q15843
Applications
ELISA, FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3U14
Quantitative proteomics reveals cellular responses to individual mAb expression and tunicamycin in CHO cells., PMID:38896138
Targeted degradation of oncogenic BCR-ABL by silencing the gene of NEDD8 E3 ligase RAPSYN., PMID:38741123
Whole-genome CRISPR screening identifies molecular mechanisms of PD-L1 expression in adult T-cell leukemia/lymphoma., PMID:38142436
TGF-β Score based on Silico Analysis can Robustly Predict Prognosis and Immunological Characteristics in Lower-grade Glioma: The Evidence from Multicenter Studies., PMID:37718518
Activity-based profiling of cullin-RING E3 networks by conformation-specific probes., PMID:37653169
Activity-based profiling of cullin-RING ligase networks by conformation-specific probes., PMID:36711970
A Mass Spectrometry-Based Strategy for Mapping Modification Sites for the Ubiquitin-Like Modifier NEDD8., PMID:36446972
PD-L1 induction via the MEK-JNK-AP1 axis by a neddylation inhibitor promotes cancer-associated immunosuppression., PMID:36192389
New Therapeutic Strategies for Adult Acute Myeloid Leukemia., PMID:35681786
The NEDD8-activating enzyme inhibitor MLN4924 reduces ischemic brain injury in mice., PMID:35101976
A protocol to visualize cytosolic aggresome-like bodies using confocal microscopy., PMID:34337443
Proteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation., PMID:33472076
Antibody RING-Mediated Destruction of Endogenous Proteins., PMID:32454028
Inhibition of neddylation causes meiotic arrest in mouse oocyte., PMID:31111756
Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics., PMID:31042464
Phosphorylated NUB1 distinguishes α-synuclein in Lewy bodies from that in glial cytoplasmic inclusions in multiple system atrophy., PMID:31006160
MLN4924 protects against interleukin-17A-induced pulmonary inflammation by disrupting ACT1-mediated signaling., PMID:30892082
Flavokawain B targets protein neddylation for enhancing the anti-prostate cancer effect of Bortezomib via Skp2 degradation., PMID:30885218
Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection., PMID:30462731
[Incorporation of novel agents into the treatment for acute myeloid leukemia]., PMID:30305501
Ubiquitin-Mimicking Peptides Transfer Differentiates by E1 and E2 Enzymes., PMID:30246024
Antibody-free detection of cellular neddylation dynamics of Cullin1., PMID:29733811
All-trans retinoic acid induces autophagic degradation of ubiquitin-like modifier activating enzyme 3 in acute promyelocytic leukemia cells., PMID:28849722
Assessment of Drug Sensitivity in Hematopoietic Stem and Progenitor Cells from Acute Myelogenous Leukemia and Myelodysplastic Syndrome Ex Vivo., PMID:28297583
An inhibitor of apoptosis protein antagonist T-3256336 potentiates the antitumor efficacy of the Nedd8-activating enzyme inhibitor pevonedistat (TAK-924/MLN4924)., PMID:27771247
Profiling of Signaling Proteins in Penumbra After Focal Photothrombotic Infarct in the Rat Brain Cortex., PMID:27771897
Efficacy of NEDD8 Pathway Inhibition in Preclinical Models of Poorly Differentiated, Clinically Aggressive Colorectal Cancer., PMID:27771609
Immunodepletion and Immunopurification as Approaches for CSN Research., PMID:27613030
The E3 Ubiquitin Ligase CRL4 Regulates Proliferation and Progression Through Meiosis in Chinese Mitten Crab Eriocheir sinensis., PMID:26864195
Pharmacologic Inhibition of Nedd8 Activation Enzyme Exposes CD4-Induced Epitopes within Env on Cells Expressing HIV-1., PMID:26676780
Pevonedistat, a NEDD8-activating enzyme inhibitor, is active in mantle cell lymphoma and enhances rituximab activity in vivo., PMID:26675347
A PTEN translational isoform has PTEN-like activity., PMID:26543340
Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis., PMID:24334119
Knockdown of SCF(Skp2) function causes double-parked accumulation in the nucleus and DNA re-replication in Drosophila plasmatocytes., PMID:24205363
Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis., PMID:22871113
Ubiquitin-related proteins in neuronal and glial intranuclear inclusions in intranuclear inclusion body disease., PMID:22612509
Immunohistochemical analysis of Marinesco bodies, using antibodies against proteins implicated in the ubiquitin-proteasome system, autophagy and aggresome formation., PMID:22118216
Radiosensitization of human pancreatic cancer cells by MLN4924, an investigational NEDD8-activating enzyme inhibitor., PMID:22072567
Characterization of MRFAP1 turnover and interactions downstream of the NEDD8 pathway., PMID:22038470
TRIM40 promotes neddylation of IKKγ and is downregulated in gastrointestinal cancers., PMID:21474709
Perturbation of 60 S ribosomal biogenesis results in ribosomal protein L5- and L11-dependent p53 activation., PMID:20554519
Inhibition of NEDD8-activating enzyme: a novel approach for the treatment of acute myeloid leukemia., PMID:20203261
Phosphorylation of VACM-1/Cul5 by protein kinase A regulates its neddylation and antiproliferative effect., PMID:19917606
The human Dcn1-like protein DCNL3 promotes Cul3 neddylation at membranes., PMID:19617556
Proteomic analysis in monocytes of antiphospholipid syndrome patients: deregulation of proteins related to the development of thrombosis., PMID:18759277
NEDD8 acts as a 'molecular switch' defining the functional selectivity of VHL., PMID:18323857
Accumulation of NEDD8 in neuronal and glial inclusions of neurodegenerative disorders., PMID:15634231
NEDD8 protein is involved in ubiquitinated inclusion bodies., PMID:12533840
The COP9 signalosome inhibits p27(kip1) degradation and impedes G1-S phase progression via deneddylation of SCF Cul1., PMID:11967155
A novel mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localized in the nucleolus at interphase., PMID:11029585
For research use only. Not for human or drug use.
