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Anti-TWIST1 Antibody (R3T93)

Catalog #:   RHH16301 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: Q15672
Overview

Catalog No.

RHH16301

Species reactivity

Human, Mouse, Rat, Rabbit

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:500, IHC: 1:100-1:500, WB: 1:500-1:2000

Target

TWIST1, TWIST, H-twist, bHLHa38, Twist-related protein 1, Class A basic helix-loop-helix protein 38, BHLHA38

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q15672

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3T93

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using TWIST1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using TWIST1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse kidney(A)Mouse spleen(B)Mouse liver(C)Mouse brain(D) using TWIST1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse kidney(A)Mouse spleen(B)Mouse liver(C)Mouse brain(D) using TWIST1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat kidney(A)Rat spleen(B)Rat liver(C)Rat brain(D) using TWIST1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit kidney(A)Rabbit spleen(B)Rabbit liver(C)Rabbit brain(D) using TWIST1 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
References

Advancing pancreatic cancer therapy by mesothelin-specific nanobody conjugation., PMID:40275270

Signaling Dynamics in Osteogenesis: Unraveling Therapeutic Targets for Bone Generation., PMID:39791147

Interleukin-9 promotes EMT-mediated PM2.5-induced pulmonary fibrosis by activating the STAT3 pathway., PMID:39259283

Is epithelial-mesenchymal transition related to the biological behavior of salivary gland neoplasms?, PMID:38852529

TOX, TWIST1, STAT4, and SATB1 protein expressions in early-stage mycosis fungoides., PMID:37932931

Multi-kinase compensation rescues EGFR knockout in a cell line model of head and neck squamous cell carcinoma., PMID:37844343

Integrated single-cell and spatial transcriptomics reveals heterogeneity of fibroblast and pivotal genes in psoriasis., PMID:37816883

Blockade of tumor-derived colony-stimulating factor 1 (CSF1) promotes an immune-permissive tumor microenvironment., PMID:37505292

Pirfenidone suppressed triple-negative breast cancer metastasis by inhibiting the activity of the TGF-β/SMAD pathway., PMID:36651490

Cancer-Associated Fibroblasts Promote Migration and Invasion of Non-Small Cell Lung Cancer Cells via miR-101-3p Mediated VEGFA Secretion and AKT/eNOS Pathway., PMID:34977016

Interference of p53:Twist1 interaction through competing nanobodies., PMID:34863830

NOL6 promotes the proliferation and migration of endometrial cancer cells by regulating TWIST1 expression., PMID:34607487

Enumeration, characterisation and clinicopathological significance of circulating tumour cells in patients with colorectal carcinoma., PMID:33610860

Gene expression in circulating tumor cells reveals a dynamic role of EMT and PD-L1 during osimertinib treatment in NSCLC patients., PMID:33504904

Co-Overexpression of TWIST1-CSF1 Is a Common Event in Metastatic Oral Cancer and Drives Biologically Aggressive Phenotype., PMID:33466385

Epithelial to Mesenchymal Transition (EMT) in a Laryngeal Squamous Cell Carcinoma of a Horse: Future Perspectives., PMID:33297475

Mechanically Driven Counter-Regulation of Cortical Bone Formation in Response to Sclerostin-Neutralizing Antibodies., PMID:33049076

TWIST1 upregulation affects E-cadherin expression in brain metastases., PMID:33006113

Gene Expression Profile and Acute Gene Expression Response to Sclerostin Inhibition in Osteogenesis Imperfecta Bone., PMID:32803109

Antitumor Activity of Ficus deltoidea Extract on Oral Cancer: An In Vivo Study., PMID:32104177

Twist1-Induced Epithelial Dissemination Requires Prkd1 Signaling., PMID:31676574

SEMA4C is a novel target to limit osteosarcoma growth, progression, and metastasis., PMID:31582836

Twist1 Inactivation in Dmp1-Expressing Cells Increases Bone Mass but Does Not Affect the Anabolic Response to Sclerostin Neutralization., PMID:31505764

Cancer-associated fibroblasts induce epithelial-mesenchymal transition of bladder cancer cells through paracrine IL-6 signalling., PMID:30744595

Role of epithelial-mesenchymal transition factors in the histogenesis of uterine carcinomas., PMID:30739164

Bovine lactoferrin reverses programming of epithelial-to-mesenchymal transition to mesenchymal-to-epithelial transition in oral squamous cell carcinoma., PMID:30415774

Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma., PMID:30297829

TIP60-dependent acetylation of the SPZ1-TWIST complex promotes epithelial-mesenchymal transition and metastasis in liver cancer., PMID:30154425

Clinical implication of programmed cell death-1 ligand-1 expression in tonsillar squamous cell carcinoma in association with intratumoral heterogeneity, human papillomavirus, and epithelial-to-mesenchymal transition., PMID:29634978

miR-200c inhibits TGF-β-induced-EMT to restore trastuzumab sensitivity by targeting ZEB1 and ZEB2 in gastric cancer., PMID:29302045

Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index., PMID:29197002

Selective targeting of pro-inflammatory Th1 cells by microRNA-148a-specific antagomirs in vivo., PMID:29183643

Multiplex Gene Expression Profiling of In Vivo Isolated Circulating Tumor Cells in High-Risk Prostate Cancer Patients., PMID:29122836

Expression of Epithelial-Mesenchymal Transition Regulating Transcription Factors in Head and Neck Squamous Cell Carcinomas., PMID:28982853

Human HER2 overexpressing mouse breast cancer cell lines derived from MMTV.f.HuHER2 mice: characterization and use in a model of metastatic breast cancer., PMID:28978097

Intercellular Genetic Interaction Between Irf6 and Twist1 during Craniofacial Development., PMID:28769044

CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer., PMID:28488542

TWIST1-WDR5-Hottip Regulates Hoxa9 Chromatin to Facilitate Prostate Cancer Metastasis., PMID:28484075

Anti-angiogenic treatment promotes triple-negative breast cancer invasion via vasculogenic mimicry., PMID:28278077

The importance of immunohistochemistry in the differential diagnosis of molar disease., PMID:28195254

Aminopeptidase A initiates tumorigenesis and enhances tumor cell stemness via TWIST1 upregulation in colorectal cancer., PMID:28177885

Differential expression of the epithelial mesenchymal transition factors Snail, Slug, Twist, TGF-β, and E-cadherin in ameloblastoma., PMID:27995335

Enhanced expression of the stemness-related factors OCT4, SOX15 and TWIST1 in ectopic endometrium of endometriosis patients., PMID:27881125

SUSD2 expression in high-grade serous ovarian cancer correlates with increased patient survival and defective mesothelial clearance., PMID:27775699

Dataset of TWIST1-regulated genes in the cranial mesoderm and a transcriptome comparison of cranial mesoderm and cranial neural crest., PMID:27699189

Twist1-induced epithelial-mesenchymal transition according to microsatellite instability status in colon cancer cells., PMID:27494849

Asparaginyl endopeptidase promotes the invasion and metastasis of gastric cancer through modulating epithelial-to-mesenchymal transition and analysis of their phosphorylation signaling pathways., PMID:27102302

TWIST1 Polymorphisms Predict Survival in Patients with Metastatic Colorectal Cancer Receiving First-Line Bevacizumab plus Oxaliplatin-Based Chemotherapy., PMID:26983880

Downregulation of Melanoma Cell Adhesion Molecule (MCAM/CD146) Accelerates Cellular Senescence in Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells., PMID:26941359

Expression of the Long Non-Coding RNA HOTAIR Correlates with Disease Progression in Bladder Cancer and Is Contained in Bladder Cancer Patient Urinary Exosomes., PMID:26800519

Datasheet

Document Download

Anti-TWIST1 Antibody (R3T93).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-TWIST1 Antibody (R3T93) [RHH16301]
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