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Anti-TUBB3/Beta Tubulin Antibody (R3S59)

Catalog #:   RHG56704 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: FCM, IF, WB
Accession: Q13509
Overview

Catalog No.

RHG56704

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

Tubulin beta-III, TUBB4, TUBB3, Tubulin beta-4 chain, Tubulin beta-3 chain

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13509

Applications

FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3S59

Data Image
  • Flow Cytometry
    Flow cytometric analysis of A549 cells using TUBB3 mouse mAb (green) and negative control (purple).
  • Immunofluorescence
    Immunofluorescence analysis of PANC-1 cells using TUBB3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot
    Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.
References

High Intraocular Concentration of Fibrinogen Regulates Retinal Function Via the ICAM-1 Pathway., PMID:39546292

Single-extracellular vesicle (EV) analyses validate the use of L1 Cell Adhesion Molecule (L1CAM) as a reliable biomarker of neuron-derived EVs., PMID:38868956

Regenerated Corneal Epithelium Expresses More βIII-Tubulin After Chemical Injuries Compared to Mechanical Injuries., PMID:38085248

Randomised phase II trial of weekly ixabepilone ± biweekly bevacizumab for platinum-resistant or refractory ovarian/fallopian tube/primary peritoneal cancer., PMID:35149854

Nuclear IMPDH Filaments in Human Gliomas., PMID:34498062

Modulation of the IGF1R-MTOR pathway attenuates motor neuron toxicity of human ALS SOD1G93A astrocytes., PMID:33749521

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

BET inhibition increases βIII-tubulin expression and sensitizes metastatic breast cancer in the brain to vinorelbine., PMID:32848091

Characterization of T-DM1-resistant breast cancer cells., PMID:32583565

Alpha- and beta-tubulin isotypes are differentially expressed during brain development., PMID:32293117

Monoclonal antibody AC10364 inhibits cell proliferation in 5-fluorouracil resistant hepatocellular carcinoma via apoptotic pathways., PMID:31303763

An in vitro developmental neurotoxicity screening assay for retinoic acid-induced neuronal differentiation using the human NT2/D1 cell line., PMID:30980846

Optic Nerve Head Astrocytes Display Axon-Dependent and -Independent Reactivity in Response to Acutely Elevated Intraocular Pressure., PMID:30665231

Preoperative Anti-Class III β-Tubulin Antibodies As Relevant Clinical Biomarkers in Ovarian Cancer., PMID:29448203

High protein and mRNA expression levels of TUBB3 (class III ß-tubulin) are associated with aggressive tumor features in esophageal adenocarcinomas., PMID:29383151

Post-Translational Tubulin Modifications in Human Astrocyte Cultures., PMID:28512712

Comparison of the Effects of Curcumin and RG108 on NGF-Induced PC-12 Adh Cell Differentiation and Neurite Outgrowth., PMID:28338387

Structure of neuro-endocrine and neuro-epithelial interactions in human foetal pancreas., PMID:27823763

Malignant glioma with primitive neuroectodermal tumor-like component (MG-PNET): novel microarray findings in a pediatric patient., PMID:27781423

Corneal Reinnervation and Sensation Recovery in Patients With Herpes Zoster Ophthalmicus: An In Vivo and Ex Vivo Study of Corneal Nerves., PMID:26989956

Induction of neural differentiation in rat C6 glioma cells with taxol., PMID:26665000

Human organotypic retinal cultures (HORCs) as a chronic experimental model for investigation of retinal ganglion cell degeneration., PMID:26432917

Coordinated interaction of Down syndrome cell adhesion molecule and deleted in colorectal cancer with dynamic TUBB3 mediates Netrin-1-induced axon branching., PMID:25754961

An agarose gel-based neurosphere culture system leads to enrichment of neuronal lineage cells in vitro., PMID:25539864

Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts., PMID:25343338

Tubulin β-III: a novel immunohistochemical marker for intrahepatic peripheral cholangiocarcinoma., PMID:25039376

Effect of all-trans retinoic acid on the differentiation of U87 glioma stem/progenitor cells., PMID:23852377

Autoantibodies against neuronal progenitors in sera from children with autism., PMID:23838310

Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation., PMID:23430457

Blockade of VEGFR-1 and VEGFR-2 enhances paclitaxel sensitivity in gastric cancer cells., PMID:23364970

[Immunohistochemical characteristics of human brain vascular plexuses]., PMID:23342655

Use of high content image analyses to detect chemical-mediated effects on neurite sub-populations in primary rat cortical neurons., PMID:23142577

An angiogenesis model for investigating multicellular interactions across intact microvascular networks., PMID:23125212

Overexpression of class III beta tubulin and amplified HER2 gene predict good response to paclitaxel and trastuzumab therapy., PMID:23028798

Myelin sheath decompaction, axon swelling, and functional loss during chronic secondary degeneration in rat optic nerve., PMID:22879411

[Molecular cloning of tubulin beta 3 (TUBB3) in Gekko japonicus and preparation of its polyclonal antibody]., PMID:22855447

Limiting multiple sclerosis related axonopathy by blocking Nogo receptor and CRMP-2 phosphorylation., PMID:22544872

Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia., PMID:22134676

Identification of class III β-tubulin as a marker of angiogenic perivascular cells., PMID:21958528

Disease-free survival of patients after surgical resection of non-small cell lung carcinoma and correlation with excision repair cross-complementation group 1 expression and genotype., PMID:21899657

Neural stem/progenitor cells circulating in peripheral blood of patients with neovascular form of AMD: a novel view on pathophysiology., PMID:21847578

Optineurin inclusions occur in a minority of TDP-43 positive ALS and FTLD-TDP cases and are rarely observed in other neurodegenerative disorders., PMID:21360076

Oral soft tissue alterations in patients with neurofibromatosis., PMID:21301902

Neutralization of BDNF attenuates the in vitro protective effects of olfactory ensheathing cell-conditioned medium on scratch-insulted retinal ganglion cells., PMID:21082236

Immunoreactivity for high-affinity choline transporter colocalises with VAChT in human enteric nervous system., PMID:20490865

Expression of tubulin, p53, ki67, receptors for estrogen, and progesterone in endometrial cancer., PMID:19899405

Detection of pain-related molecules in the subchondral bone of osteoarthritic knees., PMID:19730932

Brain-derived neurotrophic factor released from engineered mesenchymal stem cells attenuates glutamate- and hydrogen peroxide-mediated death of staurosporine-differentiated RGC-5 cells., PMID:19524566

Secondary retinal ganglion cell death and the neuroprotective effects of the calcium channel blocker lomerizine., PMID:19474405

[Merkel cell carcinoma--immunohistochemical study in a group of 11 patients]., PMID:19402315

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-TUBB3/Beta Tubulin Antibody (R3S59) [RHG56704]
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