Catalog No.
RHG55202
Species reactivity
Human, Mouse
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
Smad4, Mothers against decapentaplegic homolog 4, Deletion target in pancreatic carcinoma 4, DPC4, MAD homolog 4, SMAD 4, Mothers against DPP homolog 4, MADH4, SMAD4, hSMAD4, SMAD family member 4
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q13485
Applications
FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3S56
SMAD4 Deficiency Promotes Pancreatic Cancer Progression and Confers Susceptibility to TGFβ Inhibition., PMID:40440097
Pancreatic Cancer: Pathogenesis and Clinical Studies., PMID:40182139
Enhancing human NK cell antitumor function by knocking out SMAD4 to counteract TGFβ and activin A suppression., PMID:40119192
Smad4 deficiency ameliorates the progressive corneal stroma thinning caused by the loss of Tbr1., PMID:39894408
A High-Affinity Monoclonal Antibody Against the Pancreatic Ductal Adenocarcinoma Target, Anterior Gradient-2 (AGR2/PDIA17)., PMID:39727484
[Effect of Buzhong Yiqi Decoction on Treg cells in rats with autoimmune thyroiditis through TGF-β/Smad signaling pathway]., PMID:39701767
Genetic and microenvironmental evolution of colorectal liver metastases under chemotherapy., PMID:39631402
TGF-β-Induced PAUF Plays a Pivotal Role in the Migration and Invasion of Human Pancreatic Ductal Adenocarcinoma Cell Line Panc-1., PMID:39518973
The road to overcome pancreatic cancer: Where are we?, PMID:39403527
Involvement of mitogen- and stress-activated protein kinase 1 in BMP-6-induced chondrocyte differentiation., PMID:39307301
CCL9/CCR1 axis-driven chemotactic nanovesicles for attenuating metastasis of SMAD4-deficient colorectal cancer by trapping TGF-β., PMID:39220887
Clinical, genomic and immune microenvironmental determinants of nivolumab response in head and neck squamous cell carcinoma., PMID:39136017
Analysis of the DNA-binding properties of TGF-β-activated Smad complexes unveils a possible molecular basis for cellular context-dependent signaling., PMID:39114961
Echinacoside ameliorates hepatic fibrosis and tumor invasion in rats with thioacetamide-induced hepatocellular carcinoma., PMID:38461536
Adhesion, metastasis, and inhibition of cancer cells: a comprehensive review., PMID:38252369
Role of Arctiin in Fibrosis and Apoptosis in Experimentally Induced Hepatocellular Carcinoma in Rats., PMID:38205087
Sotorasib with panitumumab in chemotherapy-refractory KRASG12C-mutated colorectal cancer: a phase 1b trial., PMID:38177853
Inducible nitric oxide synthase (iNOS)-activated Cxcr2 signaling in myeloid cells promotes TGFβ-dependent squamous cell carcinoma lung metastasis., PMID:37524225
Immunohistochemical Characterization of a Large Cohort of Triple Negative Breast Cancer., PMID:37306115
Genistein ameliorated experimentally induced gastric ulcer in rats via inhibiting gastric tissues fibrosis by modulating Wnt/β-catenin/TGF-β/PKB pathway., PMID:37260037
[Hereditary hemorrhagic telangiectasia]., PMID:37062633
TGF-B1-over-expressed adipose stem cells-derived secretome exhibits CD44 suppressor and anti-cancer properties via antagonistic effects against SMAD4 in breast cancer cells., PMID:36660741
The loss of epithelial Smad4 drives immune evasion via CXCL1 while displaying vulnerability to combinatorial immunotherapy in gastric cancer., PMID:36577366
Mutational Status of SMAD4 and FBXW7 Affects Clinical Outcome in TP53-Mutated Metastatic Colorectal Cancer., PMID:36497403
Novel Antibody Exerts Antitumor Effect through Downregulation of CD147 and Activation of Multiple Stress Signals., PMID:36385956
The Detection of Immunity against WT1 and SMAD4P130L of EpCAM+ Cancer Cells in Malignant Pleural Effusion., PMID:36293034
Monitoring tumour resistance to the BRAF inhibitor combination regimen in colorectal cancer patients via circulating tumour DNA., PMID:36202008
Inhibition of lysyl oxidase‑like 2 ameliorates folic acid‑induced renal tubulointerstitial fibrosis., PMID:36168418
Selective targeting BMP2 and 4 in SMAD4 negative esophageal adenocarcinoma inhibits tumor growth and aggressiveness in preclinical models., PMID:35902550
Genomic Landscape of Primary Tumor Site and Clinical Outcome for Patients with Metastatic Colorectal Cancer Receiving Standard-of-Care Chemotherapy., PMID:35524872
Genetic Characteristics Associated With Drug Resistance in Lung Cancer and Colorectal Cancer Using Whole Exome Sequencing of Cell-Free DNA., PMID:35402275
Epidermal growth factor-like domain protein 6 recombinant protein facilitates osteogenic differentiation in adipose stem cells via bone morphogenetic protein 2/recombinant mothers against decapentaplegic homolog 4 signaling pathway., PMID:35220882
Case Report: Sarcoid-Like Reactions and Tertiary Lymphoid Structures Following Dual Checkpoint Inhibition in a Patient with Early-Stage Lung Adenocarcinoma., PMID:35173719
Bile cytology diagnosis in challenging cases: Validation of diagnostic bile cytology criteria and extensive study for immunocytochemical markers., PMID:34981669
Association between Altered Oncogenic Signaling Pathways and Overall Survival of Patients with Metastatic Colorectal Cancer., PMID:34943546
KLF4, negatively regulated by miR-7, suppresses osteoarthritis development via activating TGF-β1 signaling., PMID:34891002
An Early-Onset Advanced Rectal Cancer Patient With Increased KRAS Gene Copy Number Showed A Primary Resistance to Cetuximab in Combination With Chemotherapy: A Case Report., PMID:34888240
Successful Immunotherapy for Pancreatic Cancer in a Patient With TSC2 and SMAD4 Mutations: A Case Report., PMID:34880877
Epigenetic Modulation of Class-Switch DNA Recombination to IgA by miR-146a Through Downregulation of Smad2, Smad3 and Smad4., PMID:34868004
Cullin3 deficiency shapes tumor microenvironment and promotes cholangiocarcinoma in liver-specific Smad4/Pten mutant mice., PMID:34803491
Upfront progression under pembrolizumab followed by a complete response after encorafenib and cetuximab treatment in BRAF V600E-mutated and microsatellite unstable metastatic colorectal cancer patient: A case report., PMID:34773327
Longitudinal change of genetic variations in cetuximab-treated metastatic colorectal cancer., PMID:34315006
Comparative Analysis on Abnormal Methylome of Differentially Expressed Genes and Disease Pathways in the Immune Cells of RA and SLE., PMID:34079550
Association of SMAD4 loss with drug resistance in clinical cancer patients: A systematic meta-analysis., PMID:34048444
PD-L1 expression and surgical outcomes of adenosquamous carcinoma of the pancreas in a single-centre study of 56 lesions., PMID:33773917
Tumor-associated macrophages promote pancreatic ductal adenocarcinoma progression by inducing epithelial-to-mesenchymal transition., PMID:33428605
DEK-targeting aptamer DTA-64 attenuates bronchial EMT-mediated airway remodelling by suppressing TGF-β1/Smad, MAPK and PI3K signalling pathway in asthma., PMID:33124760
Molecular alterations and targeted therapy in pancreatic ductal adenocarcinoma., PMID:33008426
Suppressing neutrophil-dependent angiogenesis abrogates resistance to anti-VEGF antibody in a genetic model of colorectal cancer., PMID:32817421
Therapeutic Zfra4-10 or WWOX7-21 Peptide Induces Complex Formation of WWOX with Selective Protein Targets in Organs that Leads to Cancer Suppression and Spleen Cytotoxic Memory Z Cell Activation In Vivo., PMID:32764489
For research use only. Not for human or drug use.
