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Anti-ZP2 Antibody (R3R21)

Catalog #:   RHG06501 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IHC
Accession: Q05996
Overview

Catalog No.

RHG06501

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000

Target

Zona pellucida sperm-binding protein 2, ZP2, Zona pellucida glycoprotein 2, Zp-2, ZPA, Zona pellucida protein A

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q05996

Applications

ELISA, FCM, IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3R21

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using ZP2 mouse mAb (green) and negative control (red).
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ZP2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using ZP2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ZP2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using ZP2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using ZP2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat kidney tissues using ZP2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissues using ZP2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit kidney tissues using ZP2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit spinal cord tissues using ZP2 mouse mAb with DAB staining.
References

Structural basis of ZP2-targeted female nonhormonal contraception., PMID:40215272

Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice., PMID:36752682

Two Japanese pepper (Zanthoxylum piperitum) fruit-derived compounds attenuate IgE-mediated allergic response in vitro and in vivo via inhibition of mast cell degranulation., PMID:32745602

The Human Egg's Zona Pellucida., PMID:29853184

Inhibition of PSMD4 alters ZP1 ubiquitination state and sperm-oocyte-binding ability in pigs., PMID:29575084

Intracellular activation of ovastacin mediates pre-fertilization hardening of the zona pellucida., PMID:28911209

Egg Envelope Glycoproteins ZP1 and ZP3 Mediate Sperm-Egg Interaction in the Japanese Quail., PMID:32908412

The bioactivities of the central segment of Zp2 polypeptide., PMID:27193969

A structured interdomain linker directs self-polymerization of human uromodulin., PMID:26811476

Amyloid properties of the mouse egg zona pellucida., PMID:26043223

[Preparation and characterization of rabbit anti-mouse zona pellucida 2 antibodies]., PMID:25907936

Role of zona pellucida glycoproteins during fertilization in humans., PMID:25445843

Moonlighting proteins in sperm-egg interactions., PMID:25399599

Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3., PMID:24334245

Biomolecule screening for efficient attachment of biofunctionalized microparticles to the zona pellucida of mammalian oocytes and embryos., PMID:23613175

Identification of sperm head proteins involved in zona pellucida binding., PMID:23355646

Genetic and physiological study of morphologically abnormal human zona pellucida., PMID:22889493

Reversine promotes porcine muscle derived stem cells (PMDSCs) differentiation into female germ-like cells., PMID:22821411

Porcine zona pellucida glycoprotein ZP4 is responsible for the sperm-binding activity of the ZP3/ZP4 complex., PMID:22008510

Oocytic expression of zona pellucida protein ZP4 in Japanese quail (Coturnix japonica)., PMID:21729200

In humans, zona pellucida glycoprotein-1 binds to spermatozoa and induces acrosomal exocytosis., PMID:20504872

Zona pellucida protein ZP2 is expressed in the oocyte of Japanese quail (Coturnix japonica)., PMID:19846483

Binding characteristics of sperm with recombinant human zona pellucida glycoprotein-3 coated beads., PMID:19700799

Bacterial ghosts as a delivery system for zona pellucida-2 fertility control vaccines for brushtail possums (Trichosurus vulpecula)., PMID:18948157

Production of monoclonal antibodies against recombinant human zona pellucida glycoproteins: utility in immunolocalization of respective zona proteins in ovarian follicles., PMID:18313762

Cellular origin of the Bufo arenarum sperm receptor gp75, a ZP2 family member: its proteolysis after fertilization., PMID:17973629

Bonnet monkey (Macaca radiata) ovaries, like human oocytes, express four zona pellucida glycoproteins., PMID:17894386

Relevance of glycosylation of human zona pellucida glycoproteins for their binding to capacitated human spermatozoa and subsequent induction of acrosomal exocytosis., PMID:17486637

Immunocontraception in mice using repeated, multi-antigen peptides: immunization with purified recombinant antigens., PMID:17474093

Immunogenicity and contraceptive potential of three infertility-relevant zona pellucida 2 epitopes in the marsupial brushtail possum (Trichosurus vulpecula)., PMID:17244744

Immunocontraceptive effects on female rabbits infected with recombinant myxoma virus expressing rabbit ZP2 or ZP3., PMID:16306421

Mapping of B cell epitopes on the zona pellucida 2 protein of a marsupial, the brushtail possum (Trichosurus vulpecula)., PMID:15685627

Recombinant human zona pellucida proteins ZP1, ZP2 and ZP3 co-expressed in a human cell line., PMID:15064827

Localization and functional importance of a conserved zona pellucida 2 protein domain in the human and bovine ovary using monoclonal anti-ZP2 peptide antibodies., PMID:14511786

[Interests and limits of immunocontraception]., PMID:14499726

Expression in Escherichia coli and immunological characterization of three zona pellucida proteins (ZP1, ZP2, and ZP3) from a marsupial, the brushtail possum (Trichosurus vulpecula)., PMID:12506345

Localization of species conserved zona pellucida antigens in mammalian ovaries., PMID:12470573

Evaluation of the immunocontraceptive potential of Escherichia coli-expressed recombinant dog ZP2 and ZP3 in a homologous animal model., PMID:12052239

Subcellular distribution of ZP1, ZP2, and ZP3 glycoproteins during folliculogenesis and demonstration of their topographical disposition within the zona matrix of mouse ovarian oocytes., PMID:11906903

Analysis of recombinant mouse zona pellucida protein 2 (ZP2) constructs for immunocontraception., PMID:11858857

Essential role of ZP molecules in tubal transport of embryos in mice., PMID:11835578

A mouse acrosomal cortical matrix protein, MC41, has ZP2-binding activity and forms a complex with a 75-kDa serine protease., PMID:11784014

Distinct membrane fractions from mouse sperm bind different zona pellucida glycoproteins., PMID:11751265

The ovary as an immune target., PMID:11223372

Molecular and immunological approaches to mammalian fertilization., PMID:10924747

Cytochemical demonstration of modification of carbohydrates in the mouse zona pellucida during folliculogenesis., PMID:10817675

Expression of recombinant human zona pellucida protein 2 and its binding capacity to spermatozoa., PMID:10570015

Mapping of dominant B-cell epitopes of a human zona pellucida protein (ZP1)., PMID:10569978

The zona pellucida 'receptors' ZP1, ZP2 and ZP3., PMID:10526650

Inhibition of bovine sperm-oocyte fusion by a monoclonal antibody recognising the TEC-2 epitope on bovine oocytes., PMID:10471477

Datasheet

Document Download

Anti-ZP2 Antibody (R3R21).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-ZP2 Antibody (R3R21) [RHG06501]
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