Catalog No.
RHF90101
Species reactivity
Human, Mouse
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
SPIB, Transcription factor Spi-B
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q01892
Applications
ELISA, FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3Q50
The ETS-family transcription factor PU.1 is a critical regulator of the inhibitory Fcγ receptor IIB expression in humans., PMID:40420414
Blockade of OX40/OX40L signaling using anti-OX40L alleviates murine lupus nephritis., PMID:38798163
SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cells., PMID:37965309
Application of spatial transcriptomics analysis using the Visium system for the mouse nasal cavity after intranasal vaccination., PMID:37545501
Expression and Clinical Significance of Spi-B in B-cell Acute Lymphoblastic Leukemia., PMID:36169277
Activation-induced cytidine deaminase localizes to G-quadruplex motifs at mutation hotspots in lymphoma., PMID:33094287
Opposing Roles for the Related ETS-Family Transcription Factors Spi-B and Spi-C in Regulating B Cell Differentiation and Function., PMID:32457757
Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB., PMID:29127283
Increased levels of the T-helper 22-associated cytokine (interleukin-22) and transcription factor (aryl hydrocarbon receptor) in patients with periodontitis are associated with osteoclast resorptive activity and severity of the disease., PMID:28393368
Allograft inflammatory factor 1 is a regulator of transcytosis in M cells., PMID:28224999
PU.1 Regulates Ig Light Chain Transcription and Rearrangement in Pre-B Cells during B Cell Development., PMID:28062693
Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation., PMID:25733685
Mucosal Immunosenescence in the Gastrointestinal Tract: A Mini-Review., PMID:25531743
MiR-126: a novel route for natalizumab action?, PMID:24598267
A myelopoiesis gene signature during remission in anti-neutrophil cytoplasm antibody-associated vasculitis does not predict relapses but seems to reflect ongoing prednisolone therapy., PMID:24215168
Human Polyomavirus JC monitoring and noncoding control region analysis in dynamic cohorts of individuals affected by immune-mediated diseases under treatment with biologics: an observational study., PMID:24079660
The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model., PMID:23439650
Regulation of B cell linker protein transcription by PU.1 and Spi-B in murine B cell acute lymphoblastic leukemia., PMID:22956576
Polyomavirus JC reactivation and noncoding control region sequence analysis in pediatric Crohn's disease patients treated with infliximab., PMID:21547609
Sp1 binds to the G allele of the-1087 polymorphism in the IL-10 promoter and promotes IL-10 mRNA transcription and protein production., PMID:20072143
Initiation of antigen receptor-dependent differentiation into plasma cells by calmodulin inhibition of E2A., PMID:19553523
Transgenic expression of Spi-C impairs B-cell development and function by affecting genes associated with BCR signaling., PMID:18792411
Spi-B inhibits human plasma cell differentiation by repressing BLIMP1 and XBP-1 expression., PMID:18552212
Lamina propria c-kit+ immune precursors reside in human adult intestine and differentiate into natural killer cells., PMID:17681176
Spi-C has opposing effects to PU.1 on gene expression in progenitor B cells., PMID:16887979
The Ets factor Spi-B is a direct critical target of the coactivator OBF-1., PMID:16861304
Spi-1 and Spi-B control the expression of the Grap2 gene in B cells., PMID:15936902
Digoxin-specific antibody fragments: how much and when?, PMID:15862081
Tracing the pre-B to immature B cell transition in human leukemia cells reveals a coordinated sequence of primary and secondary IGK gene rearrangement, IGK deletion, and IGL gene rearrangement., PMID:15611260
Analysis of gene expression and Ig transcription in PU.1/Spi-B-deficient progenitor B cell lines., PMID:14688320
SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus., PMID:12749910
The transcription factor Spi-B is not required for somatic hypermutation., PMID:12431391
Gene array identification of Epstein Barr virus-regulated cellular genes in EBV-converted Burkitt lymphoma cell lines., PMID:12429807
Blimp-1 orchestrates plasma cell differentiation by extinguishing the mature B cell gene expression program., PMID:12150891
Critical role of tumor necrosis factor-alpha and NF-kappa B in interferon-gamma -induced CD40 expression in microglia/macrophages., PMID:11830590
The Ets factors PU.1 and Spi-B regulate the transcription in vivo of P2Y10, a lymphoid restricted heptahelical receptor., PMID:10567398
PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction., PMID:10229183
Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development., PMID:10187812
Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B., PMID:9384589
The Ets protein Spi-B is expressed exclusively in B cells and T cells during development., PMID:8691135
Nuclear localization of a DNA-binding C-terminal domain from Balbiani ring coded secretory protein., PMID:3061809
For research use only. Not for human or drug use.
