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Anti-SMAD3 Antibody (R3P94)

Catalog #:   RHF75506 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IF, WB
Accession: P84022
Overview

Catalog No.

RHF75506

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IF: 1:200-1:1000, WB: 1:500-1:2000

Target

Mad3, JV15-2, Mothers against decapentaplegic homolog 3, SMAD family member 3, Smad3, SMAD3, MADH3, hMAD-3, SMAD 3, MAD homolog 3, hSMAD3, Mothers against DPP homolog 3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P84022

Applications

IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3P94

Data Image
  • Immunofluorescence
    Immunofluorescence analysis of NIH/3T3 cells using SMAD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot
    Western blot analysis using SMAD3 mouse mAb against A549 (1), Hela (2), Jurkat (3), PC-2 (4) and NIH/3T3 (5) cell lysate.
References

Autophagy inhibition enhances antifibrotic potential of placental mesenchymal stem cells of fetal origin via regulating TGF-β1 mediated protein degradation of HGF., PMID:40258831

Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains., PMID:39840816

Fibroblast growth factor 21 alleviated atopic march by inhibiting the differentiation of type 2 helper T cells., PMID:39798471

Addressing the unmet need in NSCLC progression with advances in second-line therapeutics., PMID:39759220

[Effect of Buzhong Yiqi Decoction on Treg cells in rats with autoimmune thyroiditis through TGF-β/Smad signaling pathway]., PMID:39701767

Single-Nucleus RNA Sequencing Reveals That Gabra6+ Neurons in Prefrontal Cortex Promote the Progression of PTSD After Shockwave-Induced TBI., PMID:39665190

Iron overload in acquired sideroblastic anemias and MDS: pathophysiology and role of chelation and luspatercept., PMID:39644054

C-reactive protein promotes diabetic kidney disease via Smad3-mediated NLRP3 inflammasome activation., PMID:39539016

Suppression of Class Switch Recombination to IgA by RASA2 and RASA3 through Inhibition of TGF-β Signaling., PMID:39451029

Allergo-immunopathology mechanism of thymol-inhibiting airway remodeling in asthmatic mice by regulating TGF-β/Smad3 pathway., PMID:39278851

The anti-mCRP199-206 antibodies aggravate tubulointerstitial lesions in lupus nephritis., PMID:39237077

Genetic variants associated with immune-mediated liver injury from checkpoint inhibitors., PMID:39185906

Exploring the therapeutic potential of Potentilla fragarioides var. major (Rosaceae) extract in Alzheimer's disease using in vitro and in vivo models: A multi-faceted approach., PMID:39179018

Analysis of the DNA-binding properties of TGF-β-activated Smad complexes unveils a possible molecular basis for cellular context-dependent signaling., PMID:39114961

Blocking TGFβR synergistically enhances anti-tumor effects of anti-PD-1 antibody in a mouse model of incomplete thermal ablation., PMID:38950456

Two prospective, multicenter studies for the identification of biomarker signatures for early detection of pulmonary hypertension (PH): The CIPHER and CIPHER-MRI studies., PMID:38868397

Activin A, a Novel Chemokine, Induces Mouse NK Cell Migration via AKT and Calcium Signaling., PMID:38727264

ID1high/activin Ahigh glioblastoma cells contribute to resistance to anti-angiogenesis therapy through malformed vasculature., PMID:38658527

Complex and variable regulation of ΔNp63 and TAp63 by TGFβ has implications for the dynamics of squamous cell epithelial to mesenchymal transition., PMID:38538801

TGF-β1 induces PD-1 expression in macrophages through SMAD3/STAT3 cooperative signaling in chronic inflammation., PMID:38441961

[The Role of TGF-β1/SMAD in Diabetic Nephropathy: Mechanisms and Research Development]., PMID:38162063

Dexamethasone alleviates pulmonary sarcoidosis by regulating the TGF-β/Smad3 signaling to promote Th17/Treg cell rebalance., PMID:38159414

Investigating dysregulation of TGF-β1/SMAD3 signaling in atopic dermatitis: a molecular and immunohistochemical analysis., PMID:38066678

Maternal diet during gestation affect prostatic tissue component in SHR/Izm offspring., PMID:38032025

Radial glia promote microglial development through integrin αVβ8 -TGFβ1 signaling., PMID:37790363

Externalized histones fuel pulmonary fibrosis via a platelet-macrophage circuit of TGFβ1 and IL-27., PMID:37756334

Oxymatrine relieves high-fructose/fat-induced obesity via reprogramming the activity of lipid metabolism-related enhancer., PMID:37600712

Chlamydia trachomatis infection regulates the expression of tetraspanins, activin-A, and inhibin-A in tubal ectopic pregnancy., PMID:37480234

Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion., PMID:37340199

S100A4-neutralizing monoclonal antibody 6B12 counteracts the established experimental skin fibrosis induced by bleomycin., PMID:37314987

Analysis of intronic SNP (rs4147358) and expression of SMAD3 gene in Atopic Dermatitis: A case-control study., PMID:37100019

Role of C-Reactive Protein in Kidney Diseases., PMID:37065607

Astrocyte-Derived Exosomes Contribute to Pathologies of Neuromyelitis Optica Spectrum Disorder in Rodent Model., PMID:36966488

Cell surface GRP78 regulates TGFβ1-mediated profibrotic responses via TSP1 in diabetic kidney disease., PMID:36909183

A therapeutic target for CKD: activin A facilitates TGFβ1 profibrotic signaling., PMID:36717814

Combination therapy with DHA and BMSCs suppressed podocyte injury and attenuated renal fibrosis by modulating the TGF-β1/Smad pathway in MN mice., PMID:36648018

Phenytoin induces connective tissue growth factor (CTGF/CCN2) production through NADPH oxidase 4-mediated latent TGFβ1 activation in human gingiva fibroblasts: Suppression by curcumin., PMID:36205057

EGF Contributes to Hypertrophy of Human Ligamentum Flavum via the TGF-β1/Smad3 Signaling Pathway., PMID:36185336

Inflammation and Oxidative Stress Induce NGF Secretion by Pulmonary Arterial Cells through a TGF-β1-Dependent Mechanism., PMID:36139373

Carvedilol ameliorates dexamethasone-induced myocardial injury in rats independent of its action on the α1-adrenergic receptor., PMID:36085425

IL-37 alleviates liver granuloma caused by Schistosoma japonicum infection by inducing alternative macrophage activation., PMID:36002836

Isolation of Swine Bone Marrow Lin-/CD45-/CD133 + Cells and Cardio-protective Effects of its Exosomes., PMID:35925437

Deep learning for de-convolution of Smad2 versus Smad3 binding sites., PMID:35858839

Urokinase-type plasminogen activator negatively regulates α-smooth muscle actin expression via Endo180 and the uPA receptor in corneal fibroblasts., PMID:35649252

Attenuation of Excess TNF-α Release in Crohn's Disease by Silencing of iRHOMs 1/2 and the Restoration of TGF-β Mediated Immunosuppression Through Modulation of TACE Trafficking., PMID:35585977

Pirfenidone mitigates TGF-β1-mediated fibrosis in an idiopathic inflammatory myositis-associated interstitial lung disease model., PMID:35504143

Dioscin exhibits protective effects on in vivo and in vitro asthma models via suppressing TGF-β1/Smad2/3 and AKT pathways., PMID:35481609

ACPA Alleviates Bleomycin-Induced Pulmonary Fibrosis by Inhibiting TGF-β-Smad2/3 Signaling-Mediated Lung Fibroblast Activation., PMID:35355726

Tumor in the Crossfire: Inhibiting TGF-β to Enhance Cancer Immunotherapy., PMID:35353346

Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells., PMID:35203627

Datasheet

Document Download

Anti-SMAD3 Antibody (R3P94).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-SMAD3 Antibody (R3P94) [RHF75506]
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