Catalog No.
RHF18501
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
WB: 1:500-1:1000
Target
Proto-oncogene Wnt-3, Proto-oncogene Int-4 homolog, INT4, WNT3
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P56703
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3N60
Amyloid-β predominant Alzheimer's disease neuropathologic change., PMID:39417691
A mechanism of action-reflective, dual cell-based bioassay for determining the bioactivity of sclerostin-neutralizing antibodies., PMID:39389544
Complement C3a and C3a Receptor Activation Mediates Podocyte Injuries in the Mechanism of Primary Membranous Nephropathy., PMID:35777783
Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells., PMID:30664649
Dkk1 KO Mice Treated with Sclerostin Antibody Have Additional Increases in Bone Volume., PMID:29845410
A83-01 inhibits TGF-β-induced upregulation of Wnt3 and epithelial to mesenchymal transition in HER2-overexpressing breast cancer cells., PMID:28337662
Expression of Wnt/β-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-β1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model., PMID:27247798
Hippocampal Wnt3a is Necessary and Sufficient for Contextual Fear Memory Acquisition and Consolidation., PMID:24904070
Inhibition of Wnt signaling induces cell apoptosis and suppresses cell proliferation in cholangiocarcinoma cells., PMID:23799613
Recruitment of normal stem cells to an oncogenic phenotype by noncontiguous carcinogen-transformed epithelia depends on the transforming carcinogen., PMID:23687063
Limbal dermoid epithelium shares phenotypic characteristics common to both hair epidermal and limbal epithelial stem cells., PMID:23573994
Expression of Wnt3 activates Wnt/β-catenin pathway and promotes EMT-like phenotype in trastuzumab-resistant HER2-overexpressing breast cancer cells., PMID:23071104
Culture of mouse embryonic stem cells with serum but without exogenous growth factors is sufficient to generate functional hepatocyte-like cells., PMID:21829697
Wnt3a induces myofibroblast differentiation by upregulating TGF-β signaling through SMAD2 in a β-catenin-dependent manner., PMID:21611174
Hepatitis C virus core protein activates Wnt/β-catenin signaling through multiple regulation of upstream molecules in the SMMC-7721 cell line., PMID:21340743
Wnt and Notch pathways have interrelated opposing roles on prostate progenitor cell proliferation and differentiation., PMID:21308863
Activation of β-catenin and Akt pathways by Twist are critical for the maintenance of EMT associated cancer stem cell-like characters., PMID:21284870
Intestinal lineage commitment of embryonic stem cells., PMID:20934799
Inhibition of tumorigenesis driven by different Wnt proteins requires blockade of distinct ligand-binding regions by LRP6 antibodies., PMID:20713706
N-glycosylation gene DPAGT1 is a target of the Wnt/beta-catenin signaling pathway., PMID:20693288
Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation., PMID:20639871
Opposite spectrum of activity of canonical Wnt signaling in the osteogenic context of undifferentiated and differentiated mesenchymal cells: implications for tissue engineering., PMID:20590472
Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation., PMID:19945460
Variation in WNT genes expression in different subtypes of chronic lymphocytic leukemia., PMID:19863181
The first propeller domain of LRP6 regulates sensitivity to DKK1., PMID:19477926
Transcriptional regulation of SM22alpha by Wnt3a: convergence with TGFbeta(1)/Smad signaling at a novel regulatory element., PMID:19344627
The internally truncated LRP5 receptor presents a therapeutic target in breast cancer., PMID:19158955
Measuring GSK3 expression and activity in cells., PMID:19099245
Prostate tumor progression is mediated by a paracrine TGF-beta/Wnt3a signaling axis., PMID:18724388
Breast cancer-derived Dickkopf1 inhibits osteoblast differentiation and osteoprotegerin expression: implication for breast cancer osteolytic bone metastases., PMID:18546262
Frizzled-1 is involved in the neuroprotective effect of Wnt3a against Abeta oligomers., PMID:18521822
Aortic Msx2-Wnt calcification cascade is regulated by TNF-alpha-dependent signals in diabetic Ldlr-/- mice., PMID:17932314
SFRP2 regulates cardiomyogenic differentiation by inhibiting a positive transcriptional autofeedback loop of Wnt3a., PMID:17916803
Transcriptional program induced by Wnt protein in human fibroblasts suggests mechanisms for cell cooperativity in defining tissue microenvironments., PMID:17895986
Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor., PMID:17698587
Activation of Wnt/beta-catenin pathway mediates growth and survival in B-cell progenitor acute lymphoblastic leukaemia., PMID:17614820
[Changes of Wnt-3 protein during the proliferation of endogenous neural stem cells in neonatal rats with hypoxic-ischemic brain damage after hyperbaric oxygen therapy]., PMID:17582265
Wnt3/RhoA/ROCK signaling pathway is involved in adhesion-mediated drug resistance of multiple myeloma in an autocrine mechanism., PMID:17575106
Wnt3a regulates Lef-1 expression during airway submucosal gland morphogenesis., PMID:17335794
Monoclonal antibody 7F9 recognizes rat protein homologous to human carboxypeptidase-M in developing and adult rat lung., PMID:17207026
Glucocorticoid suppresses the canonical Wnt signal in cultured human osteoblasts., PMID:15721290
Directed differentiation of embryonic stem cells into dorsal interneurons., PMID:15545303
A proliferative role for Wnt-3a in chick somites., PMID:15110715
Biochemical characterization of the Drosophila wingless signaling pathway based on RNA interference., PMID:14966281
Axin-mediated CKI phosphorylation of beta-catenin at Ser 45: a molecular switch for the Wnt pathway., PMID:12000790
Wnt/frizzled-2 signaling induces aggregation and adhesion among cardiac myocytes by increased cadherin-beta-catenin complex., PMID:10893270
WNT signaling in the control of hair growth and structure., PMID:10049570
Cytoskeletal reorganization by soluble Wnt-3a protein signalling., PMID:9893023
For research use only. Not for human or drug use.
