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Anti-RSK2/RPS6KA3 Antibody (R3M71)

Catalog #:   RHE88003 Specific References (29) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, WB
Accession: P51812
Overview

Catalog No.

RHE88003

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

Insulin-stimulated protein kinase 1, MAP kinase-activated protein kinase 1b, ISPK-1, RSK2, Ribosomal S6 kinase 2, MAPKAPK-1b, ISPK1, pp90RSK2, p90RSK3, RSK-2, 90 kDa ribosomal protein S6 kinase 3, MAPKAPK1B, S6K-alpha-3, MAPKAP kinase 1b, p90-RSK 3, RPS6KA3, MAPK-activated protein kinase 1b, Ribosomal protein S6 kinase alpha-3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P51812

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3M71

Data Image
  • Flow Cytometry
    Flow cytometric analysis of HepG2 cells using RSK2 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot
    Western blot analysis using RSK2 mouse mAb against Hela (1), MCF-7 (2), and HepG2 (3) cell lysate.
References

Efficiency of Fulvestrant Monotherapy After CDK4/6 Inhibitor Exposure: Is This a Viable Choice?, PMID:40075731

Acquisition of Letrozole Resistance Through Activation of the p38/MAPK Signaling Cascade., PMID:33517263

Involvement of RSK1 activation in malformin-enhanced cellular fibrinolytic activity., PMID:29615689

Low-molecular weight keratins with anti-skin aging activity produced by anaerobic digestion of poultry feathers with Fervidobacterium islandicum AW-1., PMID:29438785

Protein phosphatase 2Cδ/Wip1 regulates phospho-p90RSK2 activity in lesional psoriatic skin., PMID:29290690

Nerve Growth Factor Signaling from Membrane Microdomains to the Nucleus: Differential Regulation by Caveolins., PMID:28338624

Interactions between FGFR2 and RSK2-implications for breast cancer prognosis., PMID:27476168

Rsk2, the Kinase Mutated in Coffin-Lowry Syndrome, Controls Cementum Formation., PMID:26927527

Fibroblast and Epidermal Growth Factors Utilize Different Signaling Pathways to Induce Anchorage-independent Cell Transformation in JB6 Cl41 Mouse Skin Epidermal Cells., PMID:25337589

Targeting of magnolin on ERKs inhibits Ras/ERKs/RSK2-signaling-mediated neoplastic cell transformation., PMID:24031026

Cell cycle regulated phosphorylation of the telomere-associated protein TIN2., PMID:23977114

Targeting p90 ribosomal S6 kinase eliminates tumor-initiating cells by inactivating Y-box binding protein-1 in triple-negative breast cancers., PMID:22674792

Antibody microarray analyses of signal transduction protein expression and phosphorylation during porcine oocyte maturation., PMID:18484764

Extracellular receptor kinase and cAMP response element binding protein activation in the neonatal rat heart after perinatal cocaine exposure., PMID:15470197

Involvement of ERKs and mitogen- and stress-activated protein kinase in UVC-induced phosphorylation of ATF2 in JB6 cells., PMID:15192015

Phosphorylation and acetylation of histone H3 at inducible genes: two controversies revisited., PMID:15171249

The SK3 subunit of small conductance Ca2+-activated K+ channels interacts with both SK1 and SK2 subunits in a heterologous expression system., PMID:14559917

Small conductance Ca2+-activated K+ channels formed by the expression of rat SK1 and SK2 genes in HEK 293 cells., PMID:14555714

RSK2 activity is regulated by its interaction with PEA-15., PMID:12796492

Partial apamin sensitivity of human small conductance Ca2+-activated K+ channels stably expressed in Chinese hamster ovary cells., PMID:12382077

Biochemical and biological functions of the N-terminal, noncatalytic domain of extracellular signal-regulated kinase 2., PMID:11113199

Activation of RSK by UV-light: phosphorylation dynamics and involvement of the MAPK pathway., PMID:10980595

Activation of the c-fos enhancer by the erk MAP kinase pathway through two sequence elements: the c-fos AP-1 and p62TCF sites., PMID:10723128

Generation of constitutively active p90 ribosomal S6 kinase in vivo. Implications for the mitogen-activated protein kinase-activated protein kinase family., PMID:10428774

Germline mosaicism in Coffin-Lowry syndrome., PMID:9887375

Expression of mitogen-activated protein kinase pathways during postnatal development of rat heart., PMID:9779826

Epinephrine and insulin stimulate different mitogen-activated protein kinase signaling pathways in rat skeletal muscle., PMID:9753291

The inhibition of glycogen synthase kinase-3 by insulin or insulin-like growth factor 1 in the rat skeletal muscle cell line L6 is blocked by wortmannin, but not by rapamycin: evidence that wortmannin blocks activation of the mitogen-activated protein kinase pathway in L6 cells between Ras and Raf., PMID:7945242

Activation of ribosomal protein S6 kinases does not increase glycogen synthesis or glucose transport in rat adipocytes., PMID:8063749

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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For research use only. Not for human or drug use.

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Anti-RSK2/RPS6KA3 Antibody (R3M71) [RHE88003]
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