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Anti-CEBPA Antibody (R3L95)

Catalog #:   RHE68401 Specific References (32) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P49715
Overview

Catalog No.

RHE68401

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

C/EBP alpha, CCAAT/enhancer-binding protein alpha, CEBPA, CEBP

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P49715

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3L95

Data Image
  • Flow Cytometry
    Flow cytometric analysis of MCF-7 cells using CEBPA mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of HeLa cells using CEBPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum tissues using CEBPA mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), and HepG2 (3) cell lysate.
  • Western blot
    Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), and HepG2 (3) cell lysate.
References

TIMEPOINT, a phase 1 study combining MTL-CEBPA with pembrolizumab, supports the immunomodulatory effect of MTL-CEBPA in solid tumors., PMID:40168999

Tumor-derived CCL15 regulates RNA m6A methylation in cancer-associated fibroblasts to promote hepatocellular carcinoma growth., PMID:39734010

7-MEGA™ inhibits adipogenesis in 3T3-L1 adipocytes and suppresses obesity in high-fat-diet-induced obese C57BL/6 mice., PMID:38909257

The lncRNA expression profile signature of leukemia stem cells is altered upon PI3K/mTOR inhibition: an in vitro and in silico study., PMID:37470414

A clinicopathological experience in acute myeloid leukemia: Effects of clinical data and status of FLT3, CEBPA and NPM1 on prognosis., PMID:35900493

CEBPA mutants down-regulate AML cell susceptibility to NK-mediated lysis by disruption of the expression of NKG2D ligands, which can be restored by LSD1 inhibition., PMID:35003895

Dysregulation of PI3K and Hippo signaling pathways synergistically induces chronic pancreatitis via CTGF upregulation., PMID:34032634

Anacardic Acid Suppresses Adipogenesis Through Inhibition of the Hsp90/Akt Signaling Pathway in 3T3-L1 Preadipocytes., PMID:34009020

Molecular classification and prognosis in younger adults with acute myeloid leukemia and intermediate-risk cytogenetics treated or not by gemtuzumab ozogamycin: Final results of the GOELAMS/FILO acute myeloid leukemia 2006-intermediate-risk trial., PMID:33765335

Outcomes following hematopoietic stem cell transplantation in patients treated with standard chemotherapy with or without gemtuzumab ozogamicin for acute myeloid leukemia., PMID:33564120

The predicting role of circulating tumor DNA landscape in gastric cancer patients treated with immune checkpoint inhibitors., PMID:33126883

Single-cell RNA-Seq of follicular lymphoma reveals malignant B-cell types and coexpression of T-cell immune checkpoints., PMID:30591526

[AML in Adults - An Update on Diagnosis, Risk Classification and Therapy]., PMID:30199909

Inhibition of Casein Kinase 2 Disrupts Differentiation of Myeloid Cells in Cancer and Enhances the Efficacy of Immunotherapy in Mice., PMID:30139814

Orchestration of B lymphoid cells and their inner myeloid by Bach., PMID:26894991

Twist1 and Twist2 Contribute to Cytokine Downregulation following Chronic NOD2 Stimulation of Human Macrophages through the Coordinated Regulation of Transcriptional Repressors and Activators., PMID:26019273

Transcriptome analysis of medaka following epinecidin-1 and TH1-5 treatment of NNV infection., PMID:25449377

CCAAT/enhancer-binding protein α negatively regulates IFN-γ expression in T cells., PMID:25398328

Hematopoiesis in the equine fetal liver suggests immune preparedness., PMID:25179685

IGH@ translocations co-exist with other primary rearrangements in B-cell precursor acute lymphoblastic leukemia., PMID:24816234

Clinical impact of gene mutations and lesions detected by SNP-array karyotyping in acute myeloid leukemia patients in the context of gemtuzumab ozogamicin treatment: results of the ALFA-0701 trial., PMID:24659740

Transcription onset of genes critical in liver carcinogenesis is epigenetically regulated by methylated DNA-binding protein MBD2., PMID:23955541

C/EBPα and DEK coordinately regulate myeloid differentiation., PMID:22474248

Evaluation of prognostic factors in AML. Preface., PMID:22127310

Stromal pleiotrophin regulates repopulation behavior of hematopoietic stem cells., PMID:21791434

CD40L induces inflammation and adipogenesis in adipose cells--a potential link between metabolic and cardiovascular disease., PMID:20174757

Mutation of C/EBPalpha predisposes to the development of myeloid leukemia in a retroviral insertional mutagenesis screen., PMID:18212247

[Acute myeloid leukemia]., PMID:18079616

Fat depot-related differences in gene expression, adiponectin secretion, and insulin action and signalling in human adipocytes differentiated in vitro from precursor stromal cells., PMID:17960360

Five members of the CEBP transcription factor family are targeted by recurrent IGH translocations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL)., PMID:17170124

Overexpression of CEBPA resulting from the translocation t(14;19)(q32;q13) of human precursor B acute lymphoblastic leukemia., PMID:16873674

DNA mimics a self-protein that may be a target for some anti-DNA antibodies in systemic lupus erythematosus., PMID:7836777

Datasheet

Document Download

Anti-CEBPA Antibody (R3L95).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-CEBPA Antibody (R3L95) [RHE68401]
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