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Anti-LIMS1 Antibody (R3L69)

Catalog #:   RHE55201 Specific References (23) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, WB
Accession: P48059
Overview

Catalog No.

RHE55201

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

PINCH, Renal carcinoma antigen NY-REN-48, PINCH1, LIM and senescent cell antigen-like-containing domain protein 1, LIMS1, PINCH-1, Particularly interesting new Cys-His protein 1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P48059

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3L69

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot
    Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
References

Genomic collision at the LIMS-1 locus increases risk of cellular mediated allograft rejection in heart transplanted recipients., PMID:39642779

Coexistence of systemic sclerosis and cryopyrin-associated periodic syndrome., PMID:38940218

Circulating Autoantibody Profiling Identifies LIMS1 as a Potential Target for Pathogenic Autoimmunity in pathologic Myopia., PMID:38729610

LIMS1 risk genotype and T cell-mediated rejection in kidney transplant recipients., PMID:33909908

Genetic background and transplantation outcomes: insights from genome-wide association studies., PMID:31815792

Genomic Mismatch at LIMS1 Locus and Kidney Allograft Rejection., PMID:31091373

ILKAP, ILK and PINCH1 control cell survival of p53-wildtype glioblastoma cells after irradiation., PMID:26460618

Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures., PMID:26004008

A protocol for exosome isolation and characterization: evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods., PMID:25820723

Galectin 9 and PINCH, novel immunotherapy targets of renal cell carcinoma: a rationale to find potential tumour antigens and the resulting cytotoxic T lymphocytes induced by the derived peptides., PMID:24895689

Caveolin-1 and integrin β1 regulate embryonic stem cell proliferation via p38 MAPK and FAK in high glucose., PMID:21506116

Neuronal PINCH is regulated by TNF-α and is required for neurite extension., PMID:20689998

Structural basis of focal adhesion localization of LIM-only adaptor PINCH by integrin-linked kinase., PMID:19117955

Novel expression of PINCH in the central nervous system and its potential as a biomarker for human immunodeficiency virus-associated neurodegeneration., PMID:18459134

Signalling via integrins: implications for cell survival and anticancer strategies., PMID:17084981

PINCH1 regulates cell-matrix and cell-cell adhesions, cell polarity and cell survival during the peri-implantation stage., PMID:15976450

Stromal staining for PINCH is an independent prognostic indicator in colorectal cancer., PMID:15720806

Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites., PMID:12432066

The signaling adapter protein PINCH is up-regulated in the stroma of common cancers, notably at invasive edges., PMID:12216108

Characterization of PINCH-2, a new focal adhesion protein that regulates the PINCH-1-ILK interaction, cell spreading, and migration., PMID:12167643

A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading., PMID:11331308

Telomeric protein Pin2/TRF1 induces mitotic entry and apoptosis in cells with short telomeres and is down-regulated in human breast tumors., PMID:11313893

Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways., PMID:9843575

Datasheet

Document Download

Anti-LIMS1 Antibody (R3L69).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-LIMS1 Antibody (R3L69) [RHE55201]
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