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Anti-NEDD4 Antibody (R3L57)

Catalog #:   RHE49904 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: WB
Accession: P46934
Overview

Catalog No.

RHE49904

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

WB: 1:500-1:1000

Target

HECT-type E3 ubiquitin transferase NEDD4, NEDD4, KIAA0093, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, NEDD4-1, NEDD-4, Neural precursor cell expressed developmentally down-regulated protein 4

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P46934

Applications

WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3L57

Data Image
  • Western blot
    Western blot analysis using NEDD4 mouse mAb against Jurkat lysate
References

HDAC1-3 inhibition triggers NEDD4-mediated CCR2 downregulation and attenuates immunosuppression in myeloid-derived suppressor cells., PMID:39891718

Potassium Activates mTORC2-dependent SGK1 Phosphorylation to Stimulate Epithelial Sodium Channel: Role in Rapid Renal Responses to Dietary Potassium., PMID:36890646

Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer., PMID:36774408

Cx43 upregulation in HUVECs under stretch via TGF-β1 and cytoskeletal network., PMID:35350835

NEDD4L binds the proteasome and promotes autophagy and bortezomib sensitivity in multiple myeloma., PMID:35236820

A five amino acids deletion in NKCC2 of C57BL/6 mice affects analysis of NKCC2 phosphorylation but does not impact kidney function., PMID:34114742

NEDD4-like ubiquitin ligase 2 protein (NEDL2) in porcine spermatozoa, oocytes, and preimplantation embryos and its role in oocyte fertilization†., PMID:33030211

Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea., PMID:32567324

A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVβ subunits., PMID:31403402

The phosphorylation site T613 in the β-subunit of rat epithelial Na+ channel (ENaC) modulates channel inhibition by Nedd4-2., PMID:29397423

Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone., PMID:29184507

Tmbim1 is a multivesicular body regulator that protects against non-alcoholic fatty liver disease in mice and monkeys by targeting the lysosomal degradation of Tlr4., PMID:28481357

The E3 ubiquitin ligase NEDD4 is translationally upregulated and facilitates pancreatic cancer., PMID:28423617

Multi-layered control of Galectin-8 mediated autophagy during adenovirus cell entry through a conserved PPxY motif in the viral capsid., PMID:28192531

The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages., PMID:27926943

Targeting of PYK2 Synergizes with EGFR Antagonists in Basal-like TNBC and Circumvents HER3-Associated Resistance via the NEDD4-NDRG1 Axis., PMID:27793840

The Nedd4-2/Ndfip1 axis is a negative regulator of IgE-mediated mast cell activation., PMID:27786273

Ubiquitin ligase Nedd4-2 modulates Kv1.3 current amplitude and ion channel protein targeting., PMID:27146988

Renal tubular SGK1 deficiency causes impaired K+ excretion via loss of regulation of NEDD4-2/WNK1 and ENaC., PMID:27009335

Mimicking the phosphorylation of Rsp5 in PKA site T761 affects its function and cellular localization., PMID:26548973

HER3/ErbB3, an emerging cancer therapeutic target., PMID:26496898

Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication., PMID:25785312

Regulation of ERBB3/HER3 signaling in cancer., PMID:25400118

TCR stimulation without co-stimulatory signals induces expression of "tolerogenic" genes in memory CD4 T cells but does not compromise cell proliferation., PMID:25306961

Expression of ENaC, SGK1 and Nedd4 isoforms in the cochlea of guinea pig., PMID:25007182

Abnormal splicing of NEDD4 in myotonic dystrophy type 2: possible link to statin adverse reactions., PMID:24907641

The E3 ubiquitin ligase NEDD4 negatively regulates HER3/ErbB3 level and signaling., PMID:24662824

Nedd4-mediated increase in HIV-1 Gag and Env proteins and immunity following DNA-vaccination of BALB/c mice., PMID:24614057

The HECT type ubiquitin ligase NEDL2 is degraded by anaphase-promoting complex/cyclosome (APC/C)-Cdh1, and its tight regulation maintains the metaphase to anaphase transition., PMID:24163370

Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1., PMID:23517290

Interaction of two translational components, lysyl-tRNA synthetase and p40/37LRP, in plasma membrane promotes laminin-dependent cell migration., PMID:22751010

Septin 4, the drosophila ortholog of human CDCrel-1, accumulates in parkin mutant brains and is functionally related to the Nedd4 E3 ubiquitin ligase., PMID:22562816

Accumulation of endogenous LITAF in aggresomes., PMID:22276139

AMP-activated protein kinase downregulates Kv7.1 cell surface expression., PMID:21957902

Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase., PMID:21231794

Protein kinase C-dependent ubiquitination and clathrin-mediated endocytosis of the cationic amino acid transporter CAT-1., PMID:21212261

Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer., PMID:21039987

Spartin activates atrophin-1-interacting protein 4 (AIP4) E3 ubiquitin ligase and promotes ubiquitination of adipophilin on lipid droplets., PMID:20504295

In vivo delivery of antigens by adenovirus dodecahedron induces cellular and humoral immune responses to elicit antitumor immunity., PMID:20179681

Lysine 63-linked polyubiquitination of the dopamine transporter requires WW3 and WW4 domains of Nedd4-2 and UBE2D ubiquitin-conjugating enzymes., PMID:20051513

Nedd4-2-mediated ubiquitination facilitates processing of surfactant protein-C., PMID:19423771

The nucleocapsid region of HIV-1 Gag cooperates with the PTAP and LYPXnL late domains to recruit the cellular machinery necessary for viral budding., PMID:19282983

Expression patterns of Nedd4 isoforms and SGK1 in the rat cochlea., PMID:19051070

Interaction between syntaxin 8 and HECTd3, a HECT domain ligase., PMID:18821010

Nedd4, a human ubiquitin ligase, affects actin cytoskeleton in yeast cells., PMID:18804462

The E3 ligase Aip4/Itch ubiquitinates and targets ErbB-4 for degradation., PMID:17463226

Identification of the WW domain-interaction sites in the unstructured N-terminal domain of EBV LMP 2A., PMID:17174309

RNA interference screen reveals an essential role of Nedd4-2 in dopamine transporter ubiquitination and endocytosis., PMID:16885233

Cell survival through Trk neurotrophin receptors is differentially regulated by ubiquitination., PMID:16701206

14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels., PMID:16613846

Datasheet

Document Download

Anti-NEDD4 Antibody (R3L57).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-NEDD4 Antibody (R3L57) [RHE49904]
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