Catalog No.
RHE49904
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
WB: 1:500-1:1000
Target
HECT-type E3 ubiquitin transferase NEDD4, NEDD4, KIAA0093, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, NEDD4-1, NEDD-4, Neural precursor cell expressed developmentally down-regulated protein 4
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P46934
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3L57
HDAC1-3 inhibition triggers NEDD4-mediated CCR2 downregulation and attenuates immunosuppression in myeloid-derived suppressor cells., PMID:39891718
Potassium Activates mTORC2-dependent SGK1 Phosphorylation to Stimulate Epithelial Sodium Channel: Role in Rapid Renal Responses to Dietary Potassium., PMID:36890646
Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer., PMID:36774408
Cx43 upregulation in HUVECs under stretch via TGF-β1 and cytoskeletal network., PMID:35350835
NEDD4L binds the proteasome and promotes autophagy and bortezomib sensitivity in multiple myeloma., PMID:35236820
A five amino acids deletion in NKCC2 of C57BL/6 mice affects analysis of NKCC2 phosphorylation but does not impact kidney function., PMID:34114742
NEDD4-like ubiquitin ligase 2 protein (NEDL2) in porcine spermatozoa, oocytes, and preimplantation embryos and its role in oocyte fertilization†., PMID:33030211
Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea., PMID:32567324
A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVβ subunits., PMID:31403402
The phosphorylation site T613 in the β-subunit of rat epithelial Na+ channel (ENaC) modulates channel inhibition by Nedd4-2., PMID:29397423
Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone., PMID:29184507
Tmbim1 is a multivesicular body regulator that protects against non-alcoholic fatty liver disease in mice and monkeys by targeting the lysosomal degradation of Tlr4., PMID:28481357
The E3 ubiquitin ligase NEDD4 is translationally upregulated and facilitates pancreatic cancer., PMID:28423617
Multi-layered control of Galectin-8 mediated autophagy during adenovirus cell entry through a conserved PPxY motif in the viral capsid., PMID:28192531
The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages., PMID:27926943
Targeting of PYK2 Synergizes with EGFR Antagonists in Basal-like TNBC and Circumvents HER3-Associated Resistance via the NEDD4-NDRG1 Axis., PMID:27793840
The Nedd4-2/Ndfip1 axis is a negative regulator of IgE-mediated mast cell activation., PMID:27786273
Ubiquitin ligase Nedd4-2 modulates Kv1.3 current amplitude and ion channel protein targeting., PMID:27146988
Renal tubular SGK1 deficiency causes impaired K+ excretion via loss of regulation of NEDD4-2/WNK1 and ENaC., PMID:27009335
Mimicking the phosphorylation of Rsp5 in PKA site T761 affects its function and cellular localization., PMID:26548973
HER3/ErbB3, an emerging cancer therapeutic target., PMID:26496898
Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication., PMID:25785312
Regulation of ERBB3/HER3 signaling in cancer., PMID:25400118
TCR stimulation without co-stimulatory signals induces expression of "tolerogenic" genes in memory CD4 T cells but does not compromise cell proliferation., PMID:25306961
Expression of ENaC, SGK1 and Nedd4 isoforms in the cochlea of guinea pig., PMID:25007182
Abnormal splicing of NEDD4 in myotonic dystrophy type 2: possible link to statin adverse reactions., PMID:24907641
The E3 ubiquitin ligase NEDD4 negatively regulates HER3/ErbB3 level and signaling., PMID:24662824
Nedd4-mediated increase in HIV-1 Gag and Env proteins and immunity following DNA-vaccination of BALB/c mice., PMID:24614057
The HECT type ubiquitin ligase NEDL2 is degraded by anaphase-promoting complex/cyclosome (APC/C)-Cdh1, and its tight regulation maintains the metaphase to anaphase transition., PMID:24163370
Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1., PMID:23517290
Interaction of two translational components, lysyl-tRNA synthetase and p40/37LRP, in plasma membrane promotes laminin-dependent cell migration., PMID:22751010
Septin 4, the drosophila ortholog of human CDCrel-1, accumulates in parkin mutant brains and is functionally related to the Nedd4 E3 ubiquitin ligase., PMID:22562816
Accumulation of endogenous LITAF in aggresomes., PMID:22276139
AMP-activated protein kinase downregulates Kv7.1 cell surface expression., PMID:21957902
Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase., PMID:21231794
Protein kinase C-dependent ubiquitination and clathrin-mediated endocytosis of the cationic amino acid transporter CAT-1., PMID:21212261
Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer., PMID:21039987
Spartin activates atrophin-1-interacting protein 4 (AIP4) E3 ubiquitin ligase and promotes ubiquitination of adipophilin on lipid droplets., PMID:20504295
In vivo delivery of antigens by adenovirus dodecahedron induces cellular and humoral immune responses to elicit antitumor immunity., PMID:20179681
Lysine 63-linked polyubiquitination of the dopamine transporter requires WW3 and WW4 domains of Nedd4-2 and UBE2D ubiquitin-conjugating enzymes., PMID:20051513
Nedd4-2-mediated ubiquitination facilitates processing of surfactant protein-C., PMID:19423771
The nucleocapsid region of HIV-1 Gag cooperates with the PTAP and LYPXnL late domains to recruit the cellular machinery necessary for viral budding., PMID:19282983
Expression patterns of Nedd4 isoforms and SGK1 in the rat cochlea., PMID:19051070
Interaction between syntaxin 8 and HECTd3, a HECT domain ligase., PMID:18821010
Nedd4, a human ubiquitin ligase, affects actin cytoskeleton in yeast cells., PMID:18804462
The E3 ligase Aip4/Itch ubiquitinates and targets ErbB-4 for degradation., PMID:17463226
Identification of the WW domain-interaction sites in the unstructured N-terminal domain of EBV LMP 2A., PMID:17174309
RNA interference screen reveals an essential role of Nedd4-2 in dopamine transporter ubiquitination and endocytosis., PMID:16885233
Cell survival through Trk neurotrophin receptors is differentially regulated by ubiquitination., PMID:16701206
14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels., PMID:16613846
For research use only. Not for human or drug use.
