Catalog No.
RHE17802
Species reactivity
Human, Mouse, Rat
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000
Target
MAP kinase kinase 2, MAPK/ERK kinase 2, ERK activator kinase 2, MAPKK 2, PRKMK2, MAP2K2, MEK 2, MEK2, MKK2, Dual specificity mitogen-activated protein kinase kinase 2
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P36507
Applications
FCM, IF, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3K04
Expression profile of mRNAs and miRNAs related to mitogen-activated kinases in HaCaT cell culture treated with lipopolysaccharide a and adalimumab., PMID:38557266
Case report: The cardio-facio-cutaneous syndrome due to a novel germline mutation in MAP2K1: A multifaceted disease with immunodeficiency and short stature., PMID:36313893
The MAP2K2 Gene as Potential Diagnostic Marker in Monitoring Adalimumab Therapy of Psoriatic Arthritis., PMID:35762548
Sequential treatment failures in response to BRAF/MEK and immune checkpoint inhibitors mediated by MAP2K2 and B2M mutations in melanoma., PMID:31082388
Blockade of PDGFRβ circumvents resistance to MEK-JAK inhibition via intratumoral CD8+ T-cells infiltration in triple-negative breast cancer., PMID:30777101
Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cells., PMID:30273905
Biased signaling downstream of epidermal growth factor receptor regulates proliferative versus apoptotic response to ligand., PMID:30250119
MEK1/2 inhibition by binimetinib is effective as a single agent and potentiates the actions of Venetoclax and ABT-737 under conditions that mimic the chronic lymphocytic leukaemia (CLL) tumour microenvironment., PMID:29767411
Synthetic lethal interaction of cetuximab with MEK1/2 inhibition in NRAS-mutant metastatic colorectal cancer., PMID:27636997
Inhibition of the Binding between RGS2 and β-Tubulin Interferes with Spindle Formation and Chromosome Segregation during Mouse Oocyte Maturation In Vitro., PMID:27463806
MEK1/2 inhibitors induce interleukin-5 expression in mouse macrophages and lymphocytes., PMID:27045084
ERK reinforces actin polymerization to power persistent edge protrusion during motility., PMID:25990957
Brominated flame retardants, tetrabromobisphenol A and hexabromocyclododecane, activate mitogen-activated protein kinases (MAPKs) in human natural killer cells., PMID:25341744
Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL., PMID:24444792
Combining radiotherapy with MEK1/2, STAT5 or STAT6 inhibition reduces survival of head and neck cancer lines., PMID:24192080
Mitogen-activated protein kinase pathway in osteosarcoma., PMID:22935974
MEK2 regulates ribonucleotide reductase activity through functional interaction with ribonucleotide reductase small subunit p53R2., PMID:22895183
Importance of inducible multidrug resistance 1 expression in HL-60 cells resistant to gemtuzumab ozogamicin., PMID:22242821
Surface IgM stimulation induces MEK1/2-dependent MYC expression in chronic lymphocytic leukemia cells., PMID:22086413
Mitogen-activated protein kinase extracellular signal-regulated kinase 2 phosphorylates and promotes Pin1 protein-dependent promyelocytic leukemia protein turnover., PMID:22033920
Acidic fibroblast growth factor (FGF) potentiates glial-mediated neurotoxicity by activating FGFR2 IIIb protein., PMID:21990352
Cerebrospinal fluid stimulates leptomeningeal and meningioma cell proliferation and activation of STAT3., PMID:21971737
Liver fibrosis protects mice from acute hepatocellular injury., PMID:21945831
Novel agents in the management of pancreatic adenocarcinoma: phase I studies. Highlights from the "2011 ASCO Gastrointestinal Cancers Symposium". San Francisco, CA, USA. January 20-22, 2011., PMID:21386633
Involvement of P2X(7) purinergic receptor and MEK1/2 in interleukin-8 up-regulation by LL-37 in human gingival fibroblasts., PMID:21338358
MEK1/2 inhibitors AS703026 and AZD6244 may be potential therapies for KRAS mutated colorectal cancer that is resistant to EGFR monoclonal antibody therapy., PMID:21118963
Insulin growth factor promotes human corneal fibroblast network formation in vitro., PMID:20631235
SSeCKS/Gravin/AKAP12 inhibits cancer cell invasiveness and chemotaxis by suppressing a protein kinase C- Raf/MEK/ERK pathway., PMID:20018890
Green tea (-)-epigallocatechin gallate inhibits insulin stimulation of 3T3-L1 preadipocyte mitogenesis via the 67-kDa laminin receptor pathway., PMID:19176763
Endosomal targeting of MEK2 requires RAF, MEK kinase activity and clathrin-dependent endocytosis., PMID:18657070
Atrial natriuretic peptide and long acting natriuretic peptide inhibit MEK 1/2 activation in human prostate cancer cells., PMID:18225537
The Akt/Mcl-1 pathway plays a prominent role in mediating antiapoptotic signals downstream of the B-cell receptor in chronic lymphocytic leukemia B cells., PMID:17928528
Oncostatin M-induced effects on EMT in human proximal tubular cells: differential role of ERK signaling., PMID:17881458
Regulation of intracellular MEK1/2 translocation in mouse oocytes: cytoplasmic dynein/dynactin-mediated poleward transport and cyclin B degradation-dependent release from spindle poles., PMID:17507801
Subversion of B lymphocyte tolerance by hydralazine, a potential mechanism for drug-induced lupus., PMID:17404230
CD160-activating NK cell effector functions depend on the phosphatidylinositol 3-kinase recruitment., PMID:17307798
Global functional analysis of nucleophosmin in Taxol response, cancer, chromatin regulation, and ribosomal DNA transcription., PMID:17069796
Positive regulation of Raf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes., PMID:16239230
ERK1/2 and MEK1/2 induced by Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) early during infection of target cells are essential for expression of viral genes and for establishment of infection., PMID:16051824
Platelet-derived growth factor-BB-induced human smooth muscle cell proliferation depends on basic FGF release and FGFR-1 activation., PMID:15625285
Arrangement of expression and distribution of tight junction protein claudin-1 in cell dissociation of pancreatic cancer cells., PMID:15547692
Relationship between activation of epidermal growth factor receptor and cell dissociation in pancreatic cancer., PMID:15492819
Rituximab induces different but overlapping sets of genes in human B-lymphoma cell lines., PMID:15449038
High expression of PKC-MAPK pathway mRNAs correlates with glomerular lesions in human diabetic nephropathy., PMID:15327405
Glycogen synthase kinase-3beta is tyrosine-phosphorylated by MEK1 in human skin fibroblasts., PMID:15020233
Phosphatidylinositol 3-kinase-dependent mitogen-activated protein/extracellular signal-regulated kinase kinase 1/2 and NF-kappa B signaling pathways are required for B cell antigen receptor-mediated cyclin D2 induction in mature B cells., PMID:14978074
Use of inhibitors in the study of MAPK signaling., PMID:14755084
Phosphorylation regulates nucleophosmin targeting to the centrosome during mitosis as detected by cross-reactive phosphorylation-specific MKK1/MKK2 antibodies., PMID:14670079
Glycoprotein IIb/IIIa inhibition attenuates platelet-activating factor-induced platelet activation by reducing protein kinase C activity., PMID:12911597
Inhibition of interleukin-4-induced class switch recombination by a human immunoglobulin Fc gamma-Fc epsilon chimeric protein., PMID:12801927
For research use only. Not for human or drug use.
