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Anti-MYH9 Antibody (R3J79)

Catalog #:   RHE13203 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: IHC, IP, WB
Accession: P35579
Overview

Catalog No.

RHE13203

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

IHC: 1:50-1:250, IP: 1:100-1:200, WB: 1:500-1:1000

Target

NMMHC-IIA, Non-muscle myosin heavy chain IIa, Cellular myosin heavy chain, type A, Myosin heavy chain 9, NMMHC II-a, Non-muscle myosin heavy chain A, Myosin-9, MYH9, NMMHC-A, Myosin heavy chain, non-muscle IIa

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P35579

Applications

IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3J79

Data Image
  • Immunohistochemical
    IHC-P analysis of kidney tissue by MYH9 antibody (RHE13203). IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue;Result: Cells in glomeruli are positively stained at the cytoplasm.
  • Immunoprecipitation
    Lane 1: HeLa lysate; Lane 2: MYH9 immunoprecipitated from HeLa lysate by RHE13203;Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: RHE13203 can immunoprecipitate MYH9;
  • Western blot
    Lane 1: 15 µg of HeLa lysate; Lane 2: 15 µg of HT-29 lysate; Lane 3: 15 µg of A-431 lysate; Result: RHE13203 can detect MYH9 by Western blotting;
References

Hepatocyte-specific C-C motif chemokine ligand 9 signaling promotes liver fibrosis progression in mice., PMID:40359088

Monoclonal Antibodies Targeting Porcine Macrophages Are Able to Inhibit the Cell Entry of Macrophage-Tropic Viruses (PRRSV and ASFV)., PMID:40006922

Antibody MYH9 and Antibiotic Lidamycin Inhibit the Growth and Proliferation of Lung Cancer Cells and Induce Their Apoptosis., PMID:38997500

Aggregates of nonmuscular myosin IIA in erythrocytes associate with GATA1- and GFI1B-related thrombocytopenia., PMID:38103735

Amino acids 1811-1960 of myosin heavy chain 9 is involved in murine gammaherpesvirus 68 infection., PMID:37515945

Successful administration of eltrombopag in preparation for peritoneal dialysis catheter placement in a girl with MYH9-related disease., PMID:37000325

Renal injury associated with MYH9 disorder with 5773delG mutation: A case report., PMID:36602281

Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells., PMID:35694306

Myosin Heavy Chain 9 (MYH9)-Related Congenital Macrothrombocytopenia., PMID:34527454

circ-EIF6 encodes EIF6-224aa to promote TNBC progression via stabilizing MYH9 and activating the Wnt/beta-catenin pathway., PMID:34450253

4H12, a Murine Monoclonal Antibody Directed against Myosin Heavy Chain-9 Expressed on Acinar Cell Carcinoma of Pancreas with Potential Therapeutic Application., PMID:34425650

Immunoglobulin A nephropathy in a patient with an MYH9 -related disorder., PMID:34383333

Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu., PMID:32362501

Diagnosis of Inherited Platelet Disorders on a Blood Smear., PMID:32079152

A novel de novo MYH9 mutation in MYH9-related disease: A case report and review of literature., PMID:31977897

MYH9 Key Amino Acid Residues Identified by the Anti-Idiotypic Antibody to Porcine Reproductive and Respiratory Syndrome Virus Glycoprotein 5 Involve in the Virus Internalization by Porcine Alveolar Macrophages., PMID:31905776

The use of pan-retinal photocoagulation to treat recurrent vitreous haemorrhage with neovascularisation in the context of Epstein syndrome: an MYH9-related disorder., PMID:31888892

Next-generation sequencing for the diagnosis of MYH9-RD: Predicting pathogenic variants., PMID:31562665

MYH9 overexpression correlates with clinicopathological parameters and poor prognosis of epithelial ovarian cancer., PMID:31423165

MYH9-related disorders display heterogeneous kidney involvement and outcome., PMID:31384440

Cell surface expression of HLA I molecules as a marker of young platelets., PMID:31207003

Proteome-transcriptome analysis and proteome remodeling in mouse lens epithelium and fibers., PMID:30359574

Multiple myosin motors interact with sodium/potassium-ATPase alpha 1 subunits., PMID:30086768

TRPV4 mediates the Ca2+ influx required for the interaction between flightless-1 and non-muscle myosin, and collagen remodeling., PMID:28526784

Diagnosis of inherited platelet disorders on a blood smear: a tool to facilitate worldwide diagnosis of platelet disorders., PMID:28457011

Efficacy of neutrophil non-muscle myosin heavy chain-IIA immunofluorescence analysis in determining the pathogenicity of MYH9 variants., PMID:28293712

THE EFFECT OF LYSOPHOSPHATIDIC ACID ON THE COMPOSITION OF MYOSIN-9 AND TROPOMYOSIN CONTAINING CYTOPLASMIC PROTEIN COMPLEXES., PMID:30199160

MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection., PMID:27112594

Late onset and high-frequency dominant hearing loss in a family with MYH9 disorder., PMID:26942920

Successful Kidney Transplantation in Epstein Syndrome With Antiplatelet Antibodies and Donor-specific Antibodies: A Case Report., PMID:26518967

Genetic susceptibility to idiopathic membranous nephropathy in high-prevalence Area, Taiwan., PMID:25520922

Rituximab induced psoriasis in an infant., PMID:25424224

Myosin-II repression favors pre/proplatelets but shear activation generates platelets and fails in macrothrombocytopenia., PMID:25395423

[Recent progress in platelet laboratory testing]., PMID:25297771

Congenital thrombocytopenia with nephritis - The first case of MYH9 related disorder in Serbia., PMID:24783421

Close relations between podocyte injuries and membranous proliferative glomerulonephritis in autoimmune murine models., PMID:23817053

May-Hegglin anomaly and pregnancy: a systematic review., PMID:23811802

Clinical, pathological, and genetic analysis of ten patients with MYH9-related disease., PMID:23207509

Autoimmunity and cystatin SA1 deficiency behind chronic mucocutaneous candidiasis in autoimmune polyendocrine syndrome type 1., PMID:23122533

PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change., PMID:23083320

Collapsing glomerulopathy associated lupus in a black female with homozygous APOL1 mutation., PMID:22952321

International collaboration as a tool for diagnosis of patients with inherited thrombocytopenia in the setting of a developing country., PMID:22672365

Mouse models of MYH9-related disease: mutations in nonmuscle myosin II-A., PMID:21908426

Genetics of proteinuria: an overview of gene mutations associated with nonsyndromic proteinuric glomerulopathies., PMID:21782134

May-Hegglin anomaly in a dog., PMID:21554370

A notable case report of May-Hegglin anomaly with immune complex-related nephropathy: a genetic and histological analysis., PMID:21329637

Nonmuscle myosin IIA is required for lamellipodia formation through binding to WAVE2 and phosphatidylinositol 3,4,5-triphosphate., PMID:21184743

[Differential diagnosis and treatment of thrombocytopenia]., PMID:20941473

In vitro expression and characterization of MYH9 mutant alleles linked to hereditary hearing loss., PMID:20416459

Heavy chain myosin 9-related disease (MYH9 -RD): neutrophil inclusions of myosin-9 as a pathognomonic sign of the disorder., PMID:20174760

Datasheet

Document Download

Anti-MYH9 Antibody (R3J79).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-MYH9 Antibody (R3J79) [RHE13203]
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