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Anti-CD68 Antibody (R3J55)

Catalog #:   RHE04903 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P34810
Overview

Catalog No.

RHE04903

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

CD68, Gp110, Macrosialin

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P34810

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3J55

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using CD68 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using CD68 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CD68 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using CD68 mouse mAb against U937 (1), Hela (2), HepG2 (3), Jurkat (4) cell lysate.
  • Western blot
    Western blot analysis using CD68 mouse mAb against U937 (1), Hela (2), HepG2 (3), Jurkat (4) cell lysate.
References

Semaphorin-7A promotes macrophage-mediated mammary epithelial and ductal carcinoma in situ invasion., PMID:40470186

Crystal-Storing Histiocytosis of the Duodenum in a Patient With IgG-Lambda Multiple Myeloma., PMID:40440188

The Prognostic Impact of the Tumor Immune Microenvironment in Synovial Sarcoma: An Immunohistochemical Analysis Using Digital Pathology and Conventional Interpretation., PMID:40423041

Harnessing local and system immune profiling delineating differential responders to first-line sintilimab (anti-PD-1 antibody) combined with chemotherapy in extensive-stage small cell lung cancer: an exploratory biomarker analysis of a phase II study., PMID:40404633

Multiplatform molecular analyses reveal two molecular subgroups of NF2-related schwannomatosis vestibular schwannomas with distinct tumour microenvironment and therapeutic vulnerabilities., PMID:40343504

Tumor-associated macrophages correlate with better outcome in SHH medulloblastoma., PMID:40303994

Multiplex immunofluorescence assessment of macrophages and IL-23R in inflammatory and malignant diseases of the oral mucosa: a pilot study., PMID:40297579

Macrophage-Hosted Porphyromonas gingivalis Is a Risk Factor for Cataract Development., PMID:40266593

Hyperinflammatory repolarisation of ovarian cancer patient macrophages by anti-tumour IgE antibody, MOv18, restricts an immunosuppressive macrophage:Treg cell interaction., PMID:40210642

Cellular and immune response in fatal COVID-19 pneumonia., PMID:40190436

Production and Functional Verification of 8-Gene (GGTA1, CMAH, β4GalNT2, hCD46, hCD55, hCD59, hTBM, hCD39)-Edited Donor Pigs for Xenotransplantation., PMID:40190036

Histologic and molecular features shared between antibody-mediated rejection of kidney allografts and chronic histiocytic intervillositis support common pathogenesis., PMID:40178007

Localization of epidermal growth factor receptor-mutations using PNA:DNA probes in clinical specimens from patients with non-small cell lung cancer., PMID:40175465

Ceratonia siliqua Protects Testicular Tissue in Ischemia/Reperfusion Injury., PMID:40170082

Noninvasive Detection of Macrophages in Atherosclerotic Lesions Using a Visipaque-Based Nanoparticle Contrast Agent for Computed Tomography., PMID:40160795

Minimally invasive retrieval and characterisation of tenosynovial tissue in rheumatoid arthritis: a novel approach to study at-risk, active, and remission stages., PMID:40148202

Blockade of B-cell-activating factor may alleviate liver injury by restraining interferon-γ expression in T cells in experimental biliary atresia., PMID:40133681

An immunohistological analysis of 13 idiopathic subglottic stenosis (iSGS) excisions: a single centre study., PMID:40133356

Practical microenvironment classification in diffuse large B cell lymphoma using digital pathology., PMID:40112808

Large number of CD68+/CD163+ Hofbauer cells and characteristic vascular structural alterations in the placental villi of cases with placenta accreta spectrum., PMID:40082293

XL888 and pembrolizumab modulate the immune landscape of colorectal tumors in a phase Ib/II clinical trial., PMID:40079916

Molecular determinants of response to neoadjuvant pembrolizumab plus chemotherapy in patients with high-risk, early-stage, triple-negative breast cancer: exploratory analysis of the open-label, multicohort phase 1b KEYNOTE-173 study., PMID:40069763

[Morphological changes of eosinophilic coarse granules in epithelial cytoplasm of breast adenosis]., PMID:40032414

Tumor Microenvironment Modulation by Tumor-Associated Macrophages: Implications for Neoadjuvant Chemotherapy Response in Breast Cancer., PMID:40008212

Host-Microbe Interactions in Healthy and CSOM-Affected Middle Ears., PMID:40005706

Rat Hemolymph Nodes Provide a Direct Communication Site for Macrophages Interacting with Erythrocytes and Mast Cells., PMID:39999804

A Phase 2 Study of Tislelizumab as Neoadjuvant Treatment of Cisplatin-Ineligible High-Risk Upper Tract Urothelial Carcinoma., PMID:39999445

Inflammatory myopathy with abundant macrophage [IMAM]: Systemic analysis and pathological approach to distinguish it from dermatomyositis., PMID:39973451

CD47-blocking antibody interferes with neutrophil extracellular traps formation after spinal cord injury to reduce spinal cord edema., PMID:39951937

Adhered macrophages as an additional marker of cardiomyocyte injury in biopsies of patients with dilated cardiomyopathy., PMID:39927162

Human hypofunctional NCF1 variants promote pulmonary fibrosis in the bleomycin-induced mouse model and patients with systemic sclerosis via expansion of SPP1+ monocytes-derived macrophages., PMID:39919902

Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles., PMID:39897799

A multimodal imaging pipeline to decipher cell-specific metabolic functions and tissue microenvironment dynamics., PMID:39880930

Clinico-Pathological Significance of Tumor Infiltrating Immune Cells in Oral Squamous Cell Carcinoma-Hope or Hype?, PMID:39865357

Intestinal obstruction caused by disseminated mycobacterium avium complex disease following solid organ transplantation: a case report., PMID:39863833

Multiplex immunohistochemistry to explore the tumor immune microenvironment in HCC patients with different GPC3 expression., PMID:39838375

Immunotherapeutic targeting of aging-associated isoDGR motif in chronic lung inflammation., PMID:39757428

Tocilizumab does not ameliorate inflammation-induced left ventricular dysfunction in a collagen-induced arthritis rat model., PMID:39734025

Developing a 3D bone model of osteosarcoma to investigate cancer mechanisms and evaluate treatments., PMID:39724514

Efficacy of Combined Encorafenib and Binimetinib Treatment for Erdheim-Chester Disease Harboring Concurrent BRAFV600E and KRASG12R Mutations: A Case Report., PMID:39724464

CXCL12-Targeted Immunomodulatory Gene Therapy Reduces Radiation-Induced Fibrosis in Healthy Tissues., PMID:39666014

CXCR4-targeted sensitive magnetic particle imaging for abdominal aortic aneurysm early detection and prognosis evaluation by recognizing total inflammatory cells., PMID:39658102

Prevalence of hybrid TLR4+M2 monocytes/macrophages in peripheral blood and lung of systemic sclerosis patients with interstitial lung disease., PMID:39635531

An immune suppressive tumor microenvironment in primary prostate cancer promotes tumor immune escape., PMID:39602457

Gene Expression Profiling of the Peritumoral Immune Cell Infiltrate of Penile Squamous Cell Carcinomas., PMID:39596210

Conventional (bone-type) giant cell tumor of the larynx: the first case with proven H3-3A: c.103G >T (p.Gly35Trp) mutation., PMID:39565412

T-cells are significantly reduced in the luminal gastrointestinal tract of patients with "complete" 22q11.2 deletion syndrome (DiGeorge syndrome): Utilization of chromogenic multiplex immunohistochemistry to define cellular populations., PMID:39549523

Immune response and bacterial resistance of Oreochromis niloticus against bacterial fish pathogen with saffron diet., PMID:39545187

Expression of SIGLEC15 correlates with tumor immune infiltration, molecular subtypes, and breast cancer progression., PMID:39541298

Inhibition of GDNF-Driven Macrophage-to-Myofibroblast Transition Protects Against Colitis-Associated Intestinal Fibrosis., PMID:39500861

Datasheet

Document Download

Anti-CD68 Antibody (R3J55).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-CD68 Antibody (R3J55) [RHE04903]
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