Anti-SHC1 Antibody (R3H76)

Catalog No.

RHD82901

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

SH2 domain protein C1, SHC, SHC-transforming protein 1, Src homology 2 domain-containing-transforming protein C1, SHCA, SHC-transforming protein 3, SHC-transforming protein A, SHC1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P29353

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3H76

Leukemic cell-secreted interleukin-9 suppresses cytotoxic T cell-mediated killing in chronic lymphocytic leukemia., PMID:38360867

Oncostatin M: Dual Regulator of the Skeletal and Hematopoietic Systems., PMID:38198032

Temporal Modulation of Differential Alternative Splicing in HaCaT Human Keratinocyte Cell Line Chronically Exposed to Arsenic for up to 28 Wk., PMID:35072517

Subcellular Dynamic Immunopatterning of Cytosolic Protein Complexes on Microstructured Polymer Substrates., PMID:34652152

Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer., PMID:33990570

Protective Effects of ShcA Protein Silencing for Photothrombotic Cerebral Infarction., PMID:33242144

Glioblastoma TCGA Mesenchymal and IGS 23 Tumors are Identifiable by IHC and have an Immune-phenotype Indicating a Potential Benefit from Immunotherapy., PMID:32998960

High-Throughput Phosphotyrosine Protein Complexes Screening by Photoaffinity-Engineered Protein Scaffold-Based Forward-Phase Protein Array., PMID:31282657

The Src homology and collagen A (ShcA) adaptor protein may participate in the pathogenesis of membranous lupus nephritis., PMID:30189773

Antiphospholipid antibodies induce thrombosis by PP2A activation via apoER2-Dab2-SHC1 complex formation in endothelium., PMID:29500169

Affinity proteomic profiling of plasma for proteins associated to area-based mammographic breast density., PMID:29444691

Ectopic ILT3 controls BCR-dependent activation of Akt in B-cell chronic lymphocytic leukemia., PMID:28931525

Characterization of In Vivo Resistance to Osimertinib and JNJ-61186372, an EGFR/Met Bispecific Antibody, Reveals Unique and Consensus Mechanisms of Resistance., PMID:28830985

FcγRIIB-Independent Mechanisms Controlling Membrane Localization of the Inhibitory Phosphatase SHIP in Human B Cells., PMID:27456487

P66Shc, a key regulator of metabolism and mitochondrial ROS production, is dysregulated by mouse embryo culture., PMID:27385725

Docosahexaenoic acid prevented tumor necrosis factor alpha-induced endothelial dysfunction and senescence., PMID:26802937

Measuring p66Shc Signaling Pathway Activation and Mitochondrial Translocation in Cultured Cells., PMID:26523473

SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment., PMID:26043699

The p66ShcA adaptor protein regulates healing after myocardial infarction., PMID:25680868

ShcA regulates late stages of T cell development and peripheral CD4+ T cell numbers., PMID:25595778

MAGI-1 acts as a scaffolding molecule for NGF receptor-mediated signaling pathway., PMID:23769981

Mapping protein interactions by combining antibody affinity maturation and mass spectrometry., PMID:21704603

Polyomavirus middle T-antigen is a transmembrane protein that binds signaling proteins in discrete subcellular membrane sites., PMID:21228238

Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display., PMID:20164216

The adaptor protein p66Shc is a positive regulator in the angiogenic response induced by hypoxic T cells., PMID:19889727

Oscillatory flow-induced proliferation of osteoblast-like cells is mediated by alphavbeta3 and beta1 integrins through synergistic interactions of focal adhesion kinase and Shc with phosphatidylinositol 3-kinase and the Akt/mTOR/p70S6K pathway., PMID:19889638

Charting the molecular network of the drug target Bcr-Abl., PMID:19380743

Differential expression of receptor tyrosine kinases (RTKs) and IGF-I pathway activation in human uterine leiomyomas., PMID:18231572

The opposite effects of IL-15 and IL-21 on CLL B cells correlate with differential activation of the JAK/STAT and ERK1/2 pathways., PMID:17938255

p66(ShcA) and oxidative stress modulate myogenic differentiation and skeletal muscle regeneration after hind limb ischemia., PMID:17726026

Expression of the human beta3 integrin subunit in mouse smooth muscle cells enhances IGF-I-stimulated signaling and proliferation., PMID:17607710

ALK activation induces Shc and FRS2 recruitment: Signaling and phenotypic outcomes in PC12 cells differentiation., PMID:17274988

Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling., PMID:17222183

ZAP-70 enhances B-cell-receptor signaling despite absent or inefficient tyrosine kinase activation in chronic lymphocytic leukemia and lymphoma B cells., PMID:17038529

Pore-forming Staphylococcus aureus alpha-toxin triggers epidermal growth factor receptor-dependent proliferation., PMID:16984414

Irbesartan restores the in-vivo insulin signaling pathway leading to Akt activation in obese Zucker rats., PMID:16877964

beta1 integrins modulate p66ShcA expression and EGF-induced MAP kinase activation in fetal lung cells., PMID:16517240

PECAM-1 isoform-specific activation of MAPK/ERKs and small GTPases: implications in inflammation and angiogenesis., PMID:16440301

N-Methyl-D-Aspartic acid suppresses Akt activity through protein phosphatase in retinal ganglion cells., PMID:16402025

Insulin-like growth factor-I signaling in smooth muscle cells is regulated by ligand binding to the 177CYDMKTTC184 sequence of the beta3-subunit of alphaVbeta3., PMID:16195248

Activation of discoidin domain receptor 1 on CD14-positive bronchoalveolar lavage fluid cells induces chemokine production in idiopathic pulmonary fibrosis., PMID:15879152

Developmental regulation of p66Shc is altered by bronchopulmonary dysplasia in baboons and humans., PMID:15778491

Association of c-Src with p52Shc in mitotic NIH3T3 cells as revealed by Src-Shc binding site-specific antibodies., PMID:15713884

p66Shc expression in proliferating thyroid cells is regulated by thyrotropin receptor signaling., PMID:15705774

Enhanced expression of adaptor molecule p46 Shc in nuclei of hepatocellular carcinoma cells: study of LEC rats., PMID:15375560

Apoptotic cell death influences the signaling activity of the amyloid precursor protein through ShcA and Grb2 adaptor proteins in neuroblastoma SH-SY5Y cells., PMID:15341520

Direct demonstration of involvement of the adaptor protein ShcA in the regulation of Ca2+-induced platelet aggregation., PMID:15325286

Role of the p66Shc isoform in insulin-like growth factor I receptor signaling through MEK/Erk and regulation of actin cytoskeleton in rat myoblasts., PMID:15262993

CD9-mediated activation of the p46 Shc isoform leads to apoptosis in cancer cells., PMID:15226408

ALK receptor tyrosine kinase promotes cell growth and neurite outgrowth., PMID:15226403

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