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Anti-PSMB8 Antibody (R3H50)

Catalog #:   RHD76102 Specific References (48) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, IF, IHC, WB
Accession: P28062
Overview

Catalog No.

RHD76102

Species reactivity

Human, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Low molecular mass protein 7, Macropain subunit C13, Really interesting new gene 10 protein, RING10, LMP7, Y2, Proteasome component C13, PSMB5i, Multicatalytic endopeptidase complex subunit C13, Proteasome subunit beta-5i, Proteasome subunit beta type-8, PSMB8

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P28062

Applications

ELISA, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3H50

Data Image
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using PSMB8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human intima cancer tissues using PSMB8 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human colon tissues using PSMB8 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using PSMB8 mouse mAb against Hela (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate.
References

Immunohistochemical Identification and Assessment of the Location of Immunoproteasome Subunits LMP2 and LMP7 in Acquired Cholesteatoma., PMID:37762439

Efficacy, safety, and molecular response predictors of oral ixazomib and short-course rituximab in untreated iNHL., PMID:36385536

Myositis with sarcoplasmic inclusions in Nakajo-Nishimura syndrome: a genetic inflammatory myopathy., PMID:32144790

Bioinformatics Analysis of Potential Key Genes in Trastuzumab-Resistant Gastric Cancer., PMID:31949544

Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells., PMID:31595669

Overexpression of immunoproteasome low-molecular-mass polypeptide 7 and inhibiting role of next-generation proteasome inhibitor ONX 0912 on cell growth in glioma., PMID:31503210

Whole exome sequencing approach to childhood onset familial erythrodermic psoriasis unravels a novel mutation of CARD14 requiring unusual high doses of ustekinumab., PMID:31286971

Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens., PMID:30343902

Immunoproteasome inhibition prevents chronic antibody-mediated allograft rejection in renal transplantation., PMID:29229189

ONX-0914, a selective inhibitor of immunoproteasome, ameliorates experimental autoimmune myasthenia gravis by modulating humoral response., PMID:28844501

Can tonsillectomy modify the innate and adaptive immunity pathways involved in IgA nephropathy?, PMID:24756968

Rescue of MHC-1 antigen processing machinery by down-regulation in expression of IGF-1 in human glioblastoma cells., PMID:23526983

A putative role for the immunoproteasome in the maintenance of pluripotency in human embryonic stem cells., PMID:22532526

The immunoproteasome in steatohepatitis: its role in Mallory-Denk body formation., PMID:21256843

A novel role for the immunoproteasome in retinal function., PMID:20881299

Down-regulation of proteasomal subunit MB1 is an independent predictor of improved survival in ovarian cancer., PMID:19243813

Age-related increase in the immunoproteasome content in rat hippocampus: molecular and functional aspects., PMID:19012754

[Immune proteasomes in the developing rat spleen]., PMID:18949853

Autoimmune reactivity against the 20S-proteasome includes immunosubunits LMP2 (beta1i), MECL1 (beta2i) and LMP7 (beta5i)., PMID:18375405

The ubiquitin-proteasome system plays essential roles in presenting an 8-mer CTL epitope expressed in APC to corresponding CD8+ T cells., PMID:16569681

Proteasomes shape the repertoire of T cells participating in antigen-specific immune responses., PMID:16549793

Human leukocyte antigen and antigen processing machinery component defects in astrocytic tumors., PMID:16322289

Development and characterization of human constitutive proteasome and immunoproteasome subunit-specific monoclonal antibodies., PMID:16101829

Hepatocytes express abundant surface class I MHC and efficiently use transporter associated with antigen processing, tapasin, and low molecular weight polypeptide proteasome subunit components of antigen processing and presentation pathway., PMID:16002705

Coordinated downregulation of the antigen presentation machinery and HLA class I/beta2-microglobulin complex is responsible for HLA-ABC loss in bladder cancer., PMID:15455355

Antigen-processing machinery in human dendritic cells: up-regulation by maturation and down-regulation by tumor cells., PMID:15265880

Neuronal induction of the immunoproteasome in Huntington's disease., PMID:14684867

IFN-alpha mediates the up-regulation of HLA class I on melanoma cells without switching proteasome to immunoproteasome., PMID:14645150

Total loss of MHC class I in colorectal tumors can be explained by two molecular pathways: beta2-microglobulin inactivation in MSI-positive tumors and LMP7/TAP2 downregulation in MSI-negative tumors., PMID:12694570

Bipartite regulation of different components of the MHC class I antigen-processing machinery during dendritic cell maturation., PMID:11717192

Molecular studies and NK cell function of a new case of TAP2 homozygous human deficiency., PMID:11529920

Introduction of tapasin gene restores surface expression of HLA class I molecules, but not antigen presentation of an HIV envelope peptide in a hepatoma cell line., PMID:11444872

Accessory proteins that control the assembly of MHC molecules with peptides., PMID:11444385

TAP1 down-regulation in primary melanoma lesions: an independent marker of poor prognosis., PMID:11241306

IFN-beta mediates coordinate expression of antigen-processing genes in RSV-infected pulmonary epithelial cells., PMID:11159003

Association of immunoproteasomes with the endoplasmic reticulum., PMID:11104664

Tissue distribution of constitutive proteasomes, immunoproteasomes, and PA28 in rats., PMID:11032729

Gene transfer of human interferon gamma complementary DNA into a renal cell carcinoma line enhances MHC-restricted cytotoxic T lymphocyte recognition but suppresses non-MHC-restricted effector cell activity., PMID:10849555

Up-regulation of the proteasome subunit LMP7 in tissues of endotoxemic rats., PMID:10779048

Invasion by Salmonella typhimurium induces increased expression of the LMP, MECL, and PA28 proteasome genes and changes in the peptide repertoire of HLA-B27., PMID:9746558

Development and characterization of mouse anti-human LMP2, LMP7, TAP1 and TAP2 monoclonal antibodies., PMID:9510369

Characterization of rabbit antisera elicited with human LMP2- and LMP7-specific peptides and recombinant proteins., PMID:9458109

Intermediates in the formation of mouse 20S proteasomes: implications for the assembly of precursor beta subunits., PMID:9311996

Proteasome subunits, low-molecular-mass polypeptides 2 and 7 are hyperexpressed by target cells in autoimmune thyroid disease but not in insulin-dependent diabetes mellitus: implications for autoimmunity., PMID:9271825

Delineation of the subunit composition of human proteasomes using antisera against the major histocompatibility complex-encoded LMP2 and LMP7 subunits., PMID:8278381

Proteasome subunits encoded in the MHC are not generally required for the processing of peptides bound by MHC class I molecules., PMID:1436094

Proteasome subunits encoded by the major histocompatibility complex are not essential for antigen presentation., PMID:1299222

Alternative exon usage and processing of the major histocompatibility complex-encoded proteasome subunits., PMID:1429565

Datasheet

Document Download

Anti-PSMB8 Antibody (R3H50).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-PSMB8 Antibody (R3H50) [RHD76102]
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