Catalog No.
RHD74703
Species reactivity
Human, Mouse, Rat, Monkey
Host species
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IP: 1:20, WB: 1:500-1:1000
Target
RF-A protein 1, Replication protein A 70 kDa DNA-binding subunit, REPA1, RP-A p70, Single-stranded DNA-binding protein, RPA70, RPA1, Replication factor A protein 1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P27694
Applications
IF, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3H37
Distinct effects of sacituzumab govitecan and berzosertib on DNA damage response in ovarian cancer., PMID:39628575
RPA guides UNG to uracil in ssDNA to facilitate antibody class switching and repair of mutagenic uracil at the replication fork., PMID:38000394
Replication Protein A (RPA1, RPA2 and RPA3) expression in gastric cancer: correlation with clinicopathologic parameters and patients' survival., PMID:32862594
Pea (Pisum sativum) allergy in children: Pis s 1 is an immunodominant major pea allergen and presents IgE binding sites with potential diagnostic value., PMID:32078204
DNA-PKcs and ATM epistatically suppress DNA end resection and hyperactivation of ATR-dependent G2-checkpoint in S-phase irradiated cells., PMID:31601897
Polymorphisms in homologous recombination repair genes and the risk and survival of breast cancer., PMID:28940489
DNA Methylation Leads to DNA Repair Gene Down-Regulation and Trinucleotide Repeat Expansion in Patient-Derived Huntington Disease Cells., PMID:27182645
Prognostic factors of brain metastases from breast cancer: impact of targeted therapies., PMID:23831232
Immunofluorescent characterization of meiotic recombination in human males with variable spermatogenesis., PMID:23413139
Recruitment of Brd4 to the human papillomavirus type 16 DNA replication complex is essential for replication of viral DNA., PMID:23365439
Protein interaction and cellular localization of human CDC45., PMID:23364835
Human genetic variants of homologous recombination repair genes first found to be associated with Epstein-Barr virus antibody titers in healthy Cantonese., PMID:21792882
Replication stress induces micronuclei comprising of aggregated DNA double-strand breaks., PMID:21525980
Characterization of renal papillary antigen 1 (RPA-1), a biomarker of renal papillary necrosis., PMID:20233945
Biochemical characterisation of the SWI/SNF family member HLTF., PMID:19723507
Photoactivated DNA analogs of substrates of the nucleotide excision repair system and their interaction with proteins of NER-competent HeLa cell extract., PMID:19538122
Immunolocalization of Kim-1, RPA-1, and RPA-2 in kidney of gentamicin-, mercury-, or chromium-treated rats: relationship to renal distributions of iNOS and nitrotyrosine., PMID:18441258
DNA damage induced hyperphosphorylation of replication protein A. 1. Identification of novel sites of phosphorylation in response to DNA damage., PMID:15938632
The human stress-activated protein kin17 belongs to the multiprotein DNA replication complex and associates in vivo with mammalian replication origins., PMID:15831485
Insights into hRPA32 C-terminal domain--mediated assembly of the simian virus 40 replisome., PMID:15793585
An evolutionarily conserved target motif for immunoglobulin class-switch recombination., PMID:15531884
Inhibition of nucleotide excision repair by anti-XPA monoclonal antibodies which interfere with binding to RPA, ERCC1, and TFIIH., PMID:15358100
ATR and ATM regulate the timing of DNA replication origin firing., PMID:15220931
Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks., PMID:15180989
Functional characterization of replication protein A2 (RPA2) from Cryptosporidium parvum., PMID:15133081
Replication protein A (RPA) phosphorylation prevents RPA association with replication centers., PMID:14966274
Phosphatidyl inositol 3-kinase-like serine/threonine protein kinases (PIKKs) are required for DNA damage-induced phosphorylation of the 32 kDa subunit of replication protein A at threonine 21., PMID:14872059
Selective interactions of human kin17 and RPA proteins with chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner., PMID:12853634
A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome., PMID:12724401
RPA is an initiation factor for human chromosomal DNA replication., PMID:12626714
The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene., PMID:12509449
Ku antigen, an origin-specific binding protein that associates with replication proteins, is required for mammalian DNA replication., PMID:12393188
Heterogeneous expression and functional analysis of two distinct replication protein A large subunits from Cryptosporidium parvum., PMID:12392913
The spatio-temporal organization of DNA replication sites is identical in primary, immortalized and transformed mammalian cells., PMID:12356909
Cell cycle specific plasmid DNA replication in the nuclear extract of Saccharomyces cerevisiae: modulation by replication protein A and proliferating cell nuclear antigen., PMID:11955075
Autoimmunity to the M(r) 32,000 subunit of replication protein A in breast cancer., PMID:11895905
DNA damage by the enediyne C-1027 results in the inhibition of DNA replication by loss of replication protein A function and activation of DNA-dependent protein kinase., PMID:11724580
Disruption of replication protein A/single-stranded DNA complexes during apoptosis in HL-60 cells., PMID:11573944
RPA foci are associated with cell death after irradiation., PMID:11302763
BLM, the Bloom's syndrome protein, varies during the cell cycle in its amount, distribution, and co-localization with other nuclear proteins., PMID:11173860
Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repair., PMID:11092888
Purification and characterization of ATM from human placenta. A manganese-dependent, wortmannin-sensitive serine/threonine protein kinase., PMID:10713094
Requirement for PCNA and RPA in interstrand crosslink-induced DNA synthesis., PMID:10684938
Effect of the topoisomerase-II inhibitor etoposide on meiotic recombination in male mice., PMID:10648907
Stability and nuclear distribution of mammalian replication protein A heterotrimeric complex., PMID:10640430
Photocrosslinking locates a binding site for the large subunit of human replication protein A to the damaged strand of cisplatin-modified DNA., PMID:10454616
Post-replicative base excision repair in replication foci., PMID:10393198
Chromatin association of replication protein A., PMID:9822637
The replication protein A binding site in simian virus 40 (SV40) T antigen and its role in the initial steps of SV40 DNA replication., PMID:9811712
Characterization of ATM expression, localization, and associated DNA-dependent protein kinase activity., PMID:9725899
For research use only. Not for human or drug use.
