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Anti-RPA1 Antibody (R3H36)

Catalog #:   RHD74702 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P27694
Overview

Catalog No.

RHD74702

Species reactivity

Human, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

RF-A protein 1, Replication protein A 70 kDa DNA-binding subunit, REPA1, RP-A p70, Single-stranded DNA-binding protein, RPA70, RPA1, Replication factor A protein 1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P27694

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3H36

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using RPA1 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using RPA1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human prostate tissues using RPA1 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.
  • Western blot
    Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.
References

Distinct effects of sacituzumab govitecan and berzosertib on DNA damage response in ovarian cancer., PMID:39628575

RPA guides UNG to uracil in ssDNA to facilitate antibody class switching and repair of mutagenic uracil at the replication fork., PMID:38000394

Replication Protein A (RPA1, RPA2 and RPA3) expression in gastric cancer: correlation with clinicopathologic parameters and patients' survival., PMID:32862594

Pea (Pisum sativum) allergy in children: Pis s 1 is an immunodominant major pea allergen and presents IgE binding sites with potential diagnostic value., PMID:32078204

DNA-PKcs and ATM epistatically suppress DNA end resection and hyperactivation of ATR-dependent G2-checkpoint in S-phase irradiated cells., PMID:31601897

Polymorphisms in homologous recombination repair genes and the risk and survival of breast cancer., PMID:28940489

DNA Methylation Leads to DNA Repair Gene Down-Regulation and Trinucleotide Repeat Expansion in Patient-Derived Huntington Disease Cells., PMID:27182645

Prognostic factors of brain metastases from breast cancer: impact of targeted therapies., PMID:23831232

Immunofluorescent characterization of meiotic recombination in human males with variable spermatogenesis., PMID:23413139

Recruitment of Brd4 to the human papillomavirus type 16 DNA replication complex is essential for replication of viral DNA., PMID:23365439

Protein interaction and cellular localization of human CDC45., PMID:23364835

Human genetic variants of homologous recombination repair genes first found to be associated with Epstein-Barr virus antibody titers in healthy Cantonese., PMID:21792882

Replication stress induces micronuclei comprising of aggregated DNA double-strand breaks., PMID:21525980

Characterization of renal papillary antigen 1 (RPA-1), a biomarker of renal papillary necrosis., PMID:20233945

Biochemical characterisation of the SWI/SNF family member HLTF., PMID:19723507

Photoactivated DNA analogs of substrates of the nucleotide excision repair system and their interaction with proteins of NER-competent HeLa cell extract., PMID:19538122

Immunolocalization of Kim-1, RPA-1, and RPA-2 in kidney of gentamicin-, mercury-, or chromium-treated rats: relationship to renal distributions of iNOS and nitrotyrosine., PMID:18441258

DNA damage induced hyperphosphorylation of replication protein A. 1. Identification of novel sites of phosphorylation in response to DNA damage., PMID:15938632

The human stress-activated protein kin17 belongs to the multiprotein DNA replication complex and associates in vivo with mammalian replication origins., PMID:15831485

Insights into hRPA32 C-terminal domain--mediated assembly of the simian virus 40 replisome., PMID:15793585

An evolutionarily conserved target motif for immunoglobulin class-switch recombination., PMID:15531884

Inhibition of nucleotide excision repair by anti-XPA monoclonal antibodies which interfere with binding to RPA, ERCC1, and TFIIH., PMID:15358100

ATR and ATM regulate the timing of DNA replication origin firing., PMID:15220931

Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks., PMID:15180989

Functional characterization of replication protein A2 (RPA2) from Cryptosporidium parvum., PMID:15133081

Replication protein A (RPA) phosphorylation prevents RPA association with replication centers., PMID:14966274

Phosphatidyl inositol 3-kinase-like serine/threonine protein kinases (PIKKs) are required for DNA damage-induced phosphorylation of the 32 kDa subunit of replication protein A at threonine 21., PMID:14872059

Selective interactions of human kin17 and RPA proteins with chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner., PMID:12853634

A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome., PMID:12724401

RPA is an initiation factor for human chromosomal DNA replication., PMID:12626714

The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene., PMID:12509449

Ku antigen, an origin-specific binding protein that associates with replication proteins, is required for mammalian DNA replication., PMID:12393188

Heterogeneous expression and functional analysis of two distinct replication protein A large subunits from Cryptosporidium parvum., PMID:12392913

The spatio-temporal organization of DNA replication sites is identical in primary, immortalized and transformed mammalian cells., PMID:12356909

Cell cycle specific plasmid DNA replication in the nuclear extract of Saccharomyces cerevisiae: modulation by replication protein A and proliferating cell nuclear antigen., PMID:11955075

Autoimmunity to the M(r) 32,000 subunit of replication protein A in breast cancer., PMID:11895905

DNA damage by the enediyne C-1027 results in the inhibition of DNA replication by loss of replication protein A function and activation of DNA-dependent protein kinase., PMID:11724580

Disruption of replication protein A/single-stranded DNA complexes during apoptosis in HL-60 cells., PMID:11573944

RPA foci are associated with cell death after irradiation., PMID:11302763

BLM, the Bloom's syndrome protein, varies during the cell cycle in its amount, distribution, and co-localization with other nuclear proteins., PMID:11173860

Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repair., PMID:11092888

Purification and characterization of ATM from human placenta. A manganese-dependent, wortmannin-sensitive serine/threonine protein kinase., PMID:10713094

Requirement for PCNA and RPA in interstrand crosslink-induced DNA synthesis., PMID:10684938

Effect of the topoisomerase-II inhibitor etoposide on meiotic recombination in male mice., PMID:10648907

Stability and nuclear distribution of mammalian replication protein A heterotrimeric complex., PMID:10640430

Photocrosslinking locates a binding site for the large subunit of human replication protein A to the damaged strand of cisplatin-modified DNA., PMID:10454616

Post-replicative base excision repair in replication foci., PMID:10393198

Chromatin association of replication protein A., PMID:9822637

The replication protein A binding site in simian virus 40 (SV40) T antigen and its role in the initial steps of SV40 DNA replication., PMID:9811712

Characterization of ATM expression, localization, and associated DNA-dependent protein kinase activity., PMID:9725899

Datasheet

Document Download

Anti-RPA1 Antibody (R3H36).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-RPA1 Antibody (R3H36) [RHD74702]
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