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Anti-MCM3 Antibody (R3G73)

Catalog #:   RHD66502 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: FCM, IF, IHC, WB
Accession: P25205
Overview

Catalog No.

RHD66502

Species reactivity

Human, Rat, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IF: 1:50-1:200, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

p102, MCM3, DNA replication licensing factor MCM3, RLF subunit beta, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P25205

Applications

FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3G73

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using MCM3 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using MCM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using MCM3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using MCM3 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using MCM3 mouse mAb against PC-12 (1), HepG2 (2),Hela (3) and COS7 (4) cell lysate.
  • Western blot
    Western blot analysis using MCM3 mouse mAb against PC-12 (1), HepG2 (2),Hela (3) and COS7 (4) cell lysate.
References

Role of proliferative markers in assessing recurrences in surgical margins of oral squamous cell carcinoma: A systematic review., PMID:38304496

Inter-component immunohistochemical assessment of proliferative markers in uterine carcinosarcoma., PMID:36238851

Glucagon-Like Peptide-2 Stimulates S-Phase Entry of Intestinal Lgr5+ Stem Cells., PMID:35218981

The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in the Uterine Fibroids., PMID:34449552

Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones., PMID:33683199

Comparison of TMA Technique and Routine Whole Slide Analysis in Evaluation of Proliferative Markers Expression in Laryngeal Squamous Cell Cancer., PMID:33144432

Minichromosome Maintenance Proteins MCM-3, MCM-5, MCM-7, and Ki-67 as Proliferative Markers in Adrenocortical Tumors., PMID:30842144

Effect of daidzein and equol on DNA replication in MCF-7 cells., PMID:29346578

Morphological analysis of testicles in cats with disorders of sex development., PMID:28525341

Expression of p53 and selected proliferative markers (Ki-67, MCM3, PCNA, and topoisomerase IIα) in borderline ovarian tumors: Correlation with clinicopathological features., PMID:28493257

GAK is phosphorylated by c-Src and translocated from the centrosome to chromatin at the end of telophase., PMID:28135906

The evaluation of Ki67, p53, MCM3 and PCNA immunoexpressions at the level of the dental follicle of impacted teeth, dentigerous cysts and keratocystic odontogenic tumors., PMID:27516012

Immunoexpression of Ki-67, MCM2, and MCM3 in Ameloblastoma and Ameloblastic Carcinoma and Their Correlations with Clinical and Histopathological Patterns., PMID:26823641

Comparative analysis of markers of cell proliferation in canine mast cell tumours according to current classifications., PMID:26172171

Immunohistochemical evaluation of pulmonary lymphangioleiomyomatosis., PMID:26026095

Construction and use of a Trichinella spiralis phage display library to identify the interactions between parasite and host enterocytes., PMID:23420409

Characterization of O-GlcNAc cycling and proteomic identification of differentially O-GlcNAcylated proteins during G1/S transition., PMID:22967762

Increase of Mcm3 and Mcm4 expression in cervical squamous cell carcinomas., PMID:21077471

Replication origins are already licensed in G1 arrested unfertilized sea urchin eggs., PMID:20153311

Minichromosome maintenance proteins are useful adjuncts to differentiate between benign and malignant melanocytic skin lesions., PMID:19389522

GANP suppresses DNA recombination, measured by direct-repeat beta-galactosidase gene construct, but does not suppress the type of recombination applying to immunoglobulin genes in mammalian cells., PMID:17903179

Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies., PMID:17244605

Mouse pre-replicative complex proteins colocalise and interact with the centrosome., PMID:17157410

Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts., PMID:15668171

Cancer-associated expression of minichromosome maintenance 3 gene in several human cancers and its involvement in tumorigenesis., PMID:15623617

Autoantibodies and autoantigens in the urine of SLE patients., PMID:15621578

A hypophosphorylated form of RPA34 is a specific component of pre-replication centers., PMID:15456845

Involvement of GANP in B cell activation in T cell-dependent antigen response., PMID:12885157

The expression of Ki-67, MCM3, and p27 defines distinct subsets of proliferating, resting, and differentiated cells., PMID:11745678

PU.1 is involved in the regulation of B lineage-associated and developmental stage-dependent expression of the germinal center-associated DNA primase GANP., PMID:11641399

P1, a high mobility group-like protein is depressed in human breast adenocarcinoma., PMID:11544097

Immunohistochemical detection of cell growth fraction in formalin-fixed and paraffin-embedded murine tissue., PMID:11395376

Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis., PMID:11046155

Stepwise regulated chromatin assembly of MCM2-7 proteins., PMID:10722676

MCM proteins are associated with RNA polymerase II holoenzyme., PMID:10454562

Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization., PMID:9712829

Human protein MCM6 on HeLa cell chromatin., PMID:9516426

B24 protein stored in lampbrush spheres is involved in early cleavage in urodele amphibians., PMID:9433800

Mcm2 and Mcm3 are constitutive nuclear proteins that exhibit distinct isoforms and bind chromatin during specific cell cycle stages of Saccharomyces cerevisiae., PMID:9285827

Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs., PMID:9214647

Identification of two Xenopus laevis genes, xMCM2 and xCDC46, with sequence homology to MCM genes involved in DNA replication., PMID:8917078

Binding of human minichromosome maintenance proteins with histone H3., PMID:8798650

XMCM7, a novel member of the Xenopus MCM family, interacts with XMCM3 and colocalizes with it throughout replication., PMID:8816774

The possible involvement of replication-related proteins with a DEAD-box-like motif in cell-free DNA replication of Xenopus eggs., PMID:7626098

A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins., PMID:7601140

MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells., PMID:7760938

DNA polymerase alpha associated protein P1, a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA synthetic period., PMID:7925275

Identification of an amphibian oocyte nuclear protein as a candidate for a role in embryonic DNA replication., PMID:8179995

The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins., PMID:8265339

Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein., PMID:1549468

Datasheet

Document Download

Anti-MCM3 Antibody (R3G73).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-MCM3 Antibody (R3G73) [RHD66502]
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