Catalog No.
RHD65702
Species reactivity
Human, Mouse, Rat, Monkey
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
G-protein coupled receptor kinase 2, GRK2, ADRBK1, Beta-adrenergic receptor kinase 1, BARK1, BARK, Beta-ARK-1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P25098
Applications
ELISA, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3G70
GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA signaling in the Hedgehog cascade., PMID:39138140
Key phosphorylation sites for robust β-arrestin2 binding at the MOR revisited., PMID:39095612
Inhibition of GRK2 ameliorates the pristane-induced mouse SLE model by suppressing plasma cells differentiation., PMID:38936060
Kinome and phosphoproteome reprogramming underlies the aberrant immune responses in critically ill COVID-19 patients., PMID:38389037
Muscarinic acetylcholine receptor-mediated phosphorylation of extracellular signal-regulated kinase in HSY salivary ductal cells involves distinct signaling pathways., PMID:38336259
Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis., PMID:38024852
GRK2 differentially regulates FcεRI and MRGPRB2-mediated responses in mast cells., PMID:37063868
A novel GRK2 inhibitor alleviates experimental arthritis through restraining Th17 cell differentiation., PMID:36399825
Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis., PMID:36396948
GRK2 inhibitors, paroxetine and CCG258747, attenuate IgE-mediated anaphylaxis but activate mast cells via MRGPRX2 and MRGPRB2., PMID:36275707
Selective phosphorylation of threonine residues defines GPR84-arrestin interactions of biased ligands., PMID:35427647
Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots., PMID:35163118
GPCR kinase knockout cells reveal the impact of individual GRKs on arrestin binding and GPCR regulation., PMID:35087057
Characterization of Smoothened Phosphorylation and Activation., PMID:34562248
New phosphosite-specific antibodies to unravel the role of GRK phosphorylation in dopamine D2 receptor regulation and signaling., PMID:33859231
Hedgehog signaling in gastrointestinal carcinogenesis and the gastrointestinal tumor microenvironment., PMID:33777671
Efficient homing of antibody-secreting cells to the bone marrow requires RNA-binding protein ZFP36L1., PMID:33306108
Inhibition of G Protein-Coupled Receptor Kinase 2 Promotes Unbiased Downregulation of IGF1 Receptor and Restrains Malignant Cell Growth., PMID:33158816
CB1 Cannabinoid Receptors Stimulate Gβγ-GRK2-Mediated FAK Phosphorylation at Tyrosine 925 to Regulate ERK Activation Involving Neuronal Focal Adhesions., PMID:32655375
Agonist-induced phosphorylation bar code and differential post-activation signaling of the delta opioid receptor revealed by phosphosite-specific antibodies., PMID:32444688
Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists., PMID:30914485
Biased signaling by thyroid-stimulating hormone receptor-specific antibodies determines thyrocyte survival in autoimmunity., PMID:29363585
(-)-Epigallocatechin-3-gallate, the major green tea catechin, regulates the desensitization of β1 adrenoceptor via GRK2 in experimental heart failure., PMID:29247373
α-Hederin inhibits G protein-coupled receptor kinase 2-mediated phosphorylation of β2-adrenergic receptors., PMID:26902407
MFG-E8 inhibits neutrophil migration through αvβ₃-integrin-dependent MAP kinase activation., PMID:25936372
Monocytes/Macrophages control resolution of transient inflammatory pain., PMID:24793056
Blood, sphingosine-1-phosphate and lymphocyte migration dynamics in the spleen., PMID:24728595
Different mechanisms of homologous and heterologous μ-opioid receptor phosphorylation., PMID:24517854
Ligand- and cell-dependent determinants of internalization and cAMP modulation by delta opioid receptor (DOR) agonists., PMID:24022593
Hierarchical organization of multi-site phosphorylation at the CXCR4 C terminus., PMID:23734232
Phosphorylation of threonine 333 regulates trafficking of the human sst5 somatostatin receptor., PMID:23418396
Astrocyte GRK2 as a novel regulator of glutamate transport and brain damage., PMID:23313319
Deciphering µ-opioid receptor phosphorylation and dephosphorylation in HEK293 cells., PMID:22725608
G protein-coupled receptor kinase 2, with β-arrestin 2, impairs insulin-induced Akt/endothelial nitric oxide synthase signaling in ob/ob mouse aorta., PMID:22688330
GRK2 protein-mediated transphosphorylation contributes to loss of function of μ-opioid receptors induced by neuropeptide FF (NPFF2) receptors., PMID:22375000
GRK2-dependent S1PR1 desensitization is required for lymphocytes to overcome their attraction to blood., PMID:21960637
Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling., PMID:21659505
α1-adrenoceptor activation induces phosphorylation of β2-adrenoceptors in human prostate tissue., PMID:21371241
Interleukin-6-deficient mice resist development of experimental autoimmune cardiomyopathy induced by immunization of β1-adrenergic receptor., PMID:21334079
Tissue dependent differences in G-protein coupled receptor kinases associated with 5-HT4 receptor desensitization in the rat gastro-intestinal tract., PMID:20836992
Pasireotide and octreotide stimulate distinct patterns of sst2A somatostatin receptor phosphorylation., PMID:20051480
Site-specific phosphorylation of CXCR4 is dynamically regulated by multiple kinases and results in differential modulation of CXCR4 signaling., PMID:20048153
Normalization of GRK2 protein and mRNA measures in patients with depression predict response to antidepressants., PMID:19400981
Site specificity of agonist and second messenger-activated kinases for somatostatin receptor subtype 2A (Sst2A) phosphorylation., PMID:19389921
delta-Opioid receptor-stimulated Akt signaling in neuroblastoma x glioma (NG108-15) hybrid cells involves receptor tyrosine kinase-mediated PI3K activation., PMID:19362548
Chronic ethanol exposure increases the association of hippocampal mu-opioid receptors with G-protein receptor kinase 2., PMID:18760718
Different kinases desensitize the human delta-opioid receptor (hDOP-R) in the neuroblastoma cell line SK-N-BE upon peptidic and alkaloid agonists., PMID:18395423
Role of the carboxyl terminal di-leucine in phosphorylation and internalization of C5a receptor., PMID:18346468
Phosphorylation of the beta2-adrenergic receptor in plasma membranes by intrinsic GRK5., PMID:18034461
Calcium/calmodulin-dependent protein kinase II supports morphine antinociceptive tolerance by phosphorylation of glycosylated phosducin-like protein., PMID:18006024
For research use only. Not for human or drug use.
