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Anti-SPI1 Antibody (R3E90)

Catalog #:   RHD27302 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM
Accession: P17947
Overview

Catalog No.

RHD27302

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400

Target

31 kDa-transforming protein, SPI1, Transcription factor PU.1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P17947

Applications

ELISA, FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3E90

Data Image
  • Flow Cytometry
    Flow cytometric analysis of HeLa cells using SPI1 mouse mAb (green) and negative control (red).
References

Functional multiomics reveals genetic and pharmacologic regulation of surface CD38 in multiple myeloma., PMID:40453054

Protease-activated receptor 2 and interleukin-13 receptor α1 activation is linked to eosinophilic chronic rhinosinusitis., PMID:40074076

Spatiotemporal transcriptomics elucidates the pathogenesis of fulminant viral myocarditis., PMID:39924580

Integrative Bioinformatics Analysis and Experimental Study of NLRP12 Reveal Its Prognostic Value and Potential Functions in Ovarian Cancer., PMID:39601513

The mechanism underlying B-cell developmental dysfunction in Kawasaki disease based on single-cell transcriptomic sequencing., PMID:39507528

Macrophages Promote Atherosclerosis Development by Inhibiting CD8T Cell Apoptosis., PMID:39345351

Immunoregulatory Effect of Calcitriol on Experimental Autoimmune Encephalomyelitis (EAE) Mice., PMID:38085147

Salmonella Typhimurium-based inactivated vaccine containing a wide spectrum of bacterial antigens which mimics protein expression changes during different stages of an infection process., PMID:37141806

An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1., PMID:36706943

Blocking GM-CSF receptor α with mavrilimumab reduces infiltrating cells, pro-inflammatory markers and neoangiogenesis in ex vivo cultured arteries from patients with giant cell arteritis., PMID:35045965

Genomics of Alzheimer's disease implicates the innate and adaptive immune systems., PMID:34708251

Identification of Key Genes and Underlying Mechanisms in Acute Kawasaki Disease Based on Bioinformatics Analysis., PMID:34290221

Novel Template Plasmids pCyaA'-Kan and pCyaA'-Cam for Generation of Unmarked Chromosomal cyaA' Translational Fusion to T3SS Effectors in Salmonella., PMID:33668764

The Hha-TomB toxin-antitoxin module in Salmonella enterica serovar Typhimurium limits its intracellular survival profile and regulates host immune response., PMID:33651227

Leishmania amazonensis Promastigotes or Extracellular Vesicles Modulate B-1 Cell Activation and Differentiation., PMID:33194814

Salmonella Typhimurium Lacking YjeK as a Candidate Live Attenuated Vaccine Against Invasive Salmonella Infection., PMID:32655567

Transcription factors IRF8 and PU.1 are required for follicular B cell development and BCL6-driven germinal center responses., PMID:31000603

PU.1 Regulates Ig Light Chain Transcription and Rearrangement in Pre-B Cells during B Cell Development., PMID:28062693

A Genome-Wide siRNA Screen Implicates Spire1/2 in SipA-Driven Salmonella Typhimurium Host Cell Invasion., PMID:27627128

Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo., PMID:27342843

phoP, SPI1, SPI2 and aroA mutants of Salmonella Enteritidis induce a different immune response in chickens., PMID:26380970

RpoS-dependent expression of OsmY in Salmonella enterica serovar typhi: activation under stationary phase and SPI-2-inducing conditions., PMID:25820138

Virulence determinants of Salmonella Gallinarum biovar Pullorum identified by PCR signature-tagged mutagenesis and the spiC mutant as a candidate live attenuated vaccine., PMID:24355532

A myelopoiesis gene signature during remission in anti-neutrophil cytoplasm antibody-associated vasculitis does not predict relapses but seems to reflect ongoing prednisolone therapy., PMID:24215168

IL-10 produced by trophoblast cells inhibits phagosome maturation leading to profound intracellular proliferation of Salmonella enterica Typhimurium., PMID:23834952

Vaccination of chickens with SPI1-lon and SPI1-lon-fliC mutant of Salmonella enterica Serovar Enteritidis., PMID:23785484

Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis., PMID:23687968

SPI1 defective mutants of Salmonella enterica induce cross-protective immunity in chickens against challenge with serovars Typhimurium and Enteritidis., PMID:23684831

Membrane topology of Salmonella invasion protein SipB confers osmotolerance., PMID:22975346

Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages., PMID:22694285

Association of a protective monoclonal IgA with the O antigen of Salmonella enterica serovar Typhimurium impacts type 3 secretion and outer membrane integrity., PMID:22473607

IgG keeps virulent Salmonella from evading dendritic cell uptake., PMID:22352313

Vaccination of chickens with Salmonella Pathogenicity Island (SPI) 1 and SPI2 defective mutants of Salmonella enterica serovar Enteritidis., PMID:22300724

Immunization of chickens with Salmonella enterica subspecies enterica serovar Enteritidis pathogenicity island-2 proteins., PMID:21684090

Role of Salmonella Pathogenicity Island 1 protein IacP in Salmonella enterica serovar typhimurium pathogenesis., PMID:21263021

SopB effector protein of Salmonella Typhimurium is translocated in mesenteric lymph nodes during murine salmonellosis., PMID:21241360

Evaluation of Salmonella enterica serovar Enteritidis pathogenicity island-1 proteins as vaccine candidates against S. Enteritidis challenge in chickens., PMID:20888713

Protection of epithelial cells from Salmonella enterica serovar Enteritidis invasion by antibodies against the SPI-1 type III secretion system., PMID:20657623

Salmonella-containing vacuoles display centrifugal movement associated with cell-to-cell transfer in epithelial cells., PMID:19103768

SpvC is a Salmonella effector with phosphothreonine lyase activity on host mitogen-activated protein kinases., PMID:18284579

Entry route of Salmonella typhimurium directs the type of induced immune response., PMID:18083577

Role in virulence and protective efficacy in pigs of Salmonella enterica serovar Typhimurium secreted components identified by signature-tagged mutagenesis., PMID:17526851

Impact of Salmonella Typhimurium DT104 virulence factors invC and sseD on the onset, clinical course, colonization patterns and immune response of porcine salmonellosis., PMID:17524577

SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium are synthesized at late stages of infection in mice., PMID:17379731

Spi-1 and Spi-B control the expression of the Grap2 gene in B cells., PMID:15936902

Alterations of loci encoding PU.1, BOB1, and OCT2 transcription regulators do not correlate with their suppressed expression in Hodgkin lymphoma., PMID:15796964

Induction of potent humoral and cell-mediated immune responses by attenuated vaccinia virus vectors with deleted serpin genes., PMID:14990697

The putative gymnosperm plant defensin polypeptide (SPI1) accumulates after seed germination, is not readily released, and the SPI1 levels are reduced in Pythium dimorphum-infected spruce roots., PMID:12856937

Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems., PMID:11918798

Salmonella pathogenicity island 2-dependent macrophage death is mediated in part by the host cysteine protease caspase-1., PMID:11736994

Datasheet

Document Download

Anti-SPI1 Antibody (R3E90).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-SPI1 Antibody (R3E90) [RHD27302]
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