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Anti-DDX5 Antibody (R3E83)

Catalog #:   RHD26602 Specific References (27) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: ELISA, FCM, IHC, WB
Accession: P17844
Overview

Catalog No.

RHD26602

Species reactivity

Human, Mouse, Monkey

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

HLR1, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68, G17P1, DDX5, HELR, DEAD box protein 5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P17844

Applications

ELISA, FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3E83

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using DDX5 mouse mAb (green) and negative control (red).
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using DDX5 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using DDX5 mouse mAb against HT-29 (1), Hela (2), NIH/3T3 (3), COS7 (4), SW620 (5), Jurkat (6), A431 (7), and MCF-7 (8) cell lysate.
References

Application of the Monoclonal Autoantibody and Its Target Protein Derived from the Peripheral Blood of SLE Patients in Serological Diagnosis and Differential Diagnosis of SLE., PMID:39840886

Dynamic O-GlcNAcylation governs long-range chromatin interactions in V(D)J recombination during early B-cell development., PMID:39627609

RNA helicase DEAD-box-5 is involved in R-loop dynamics of preimplantation embryos., PMID:38419548

PARP1 controls the transcription of CD24 by ADP-ribosylating the RNA helicase DDX5 in pancreatic cancer., PMID:36584909

PUM1 Is Overexpressed in Colon Cancer Cells With Acquired Resistance to Cetuximab., PMID:34447749

Tumor suppressor DRD2 facilitates M1 macrophages and restricts NF-κB signaling to trigger pyroptosis in breast cancer., PMID:33859743

The Long Linker Region of Telomere-Binding Protein TRF2 Is Responsible for Interactions with Lamins., PMID:33804854

DDX5-targeting fully human monoclonal autoantibody inhibits proliferation and promotes differentiation of acute promyelocytic leukemia cells by increasing ROS production., PMID:32690860

Targeting 17q23 amplicon to overcome the resistance to anti-HER2 therapy in HER2+ breast cancer., PMID:30413718

Antibodies against the mono-methylated arginine-glycine repeat (MMA-RG) of the Epstein-Barr virus nuclear antigen 2 (EBNA2) identify potential cellular proteins targeted in viral transformation., PMID:28758620

Antibody-validated proteins in inflamed islets of fulminant type 1 diabetes profiled by laser-capture microdissection followed by mass spectrometry., PMID:25329145

Induction of amphiregulin by p53 promotes apoptosis via control of microRNA biogenesis in response to DNA damage., PMID:24379358

DDX6 post-transcriptionally down-regulates miR-143/145 expression through host gene NCR143/145 in cancer cells., PMID:23932921

A new monoclonal antibody, 4F2, specific for the oligodendroglial cell lineage, recognizes ATP-dependent RNA helicase Ddx54: possible association with myelin basic protein., PMID:21932369

Expanded CUG repeats Dysregulate RNA splicing by altering the stoichiometry of the muscleblind 1 complex., PMID:21900255

SAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesis., PMID:19171422

[Multifunctional nuclear protein NAP57 specifically interacts with dead RNA-helicase p68]., PMID:16708846

p54nrb is a component of the snRNP-free U1A (SF-A) complex that promotes pre-mRNA cleavage during polyadenylation., PMID:16373496

Serologic analysis of ovarian tumor antigens reveals a bias toward antigens encoded on 17q., PMID:12532422

A chicken embryo protein related to the mammalian DEAD box protein p68 is tightly associated with the highly purified protein-RNA complex of 5-MeC-DNA glycosylase., PMID:10454630

Expression of p68 protein kinase and its prognostic significance during IFN-alpha therapy in patients with carcinoid tumours., PMID:10070309

Regulation of p68 RNA helicase by calmodulin and protein kinase C., PMID:7525583

EBV/C3d receptor (CR2) interacts by its intracytoplasmic carboxy-terminal domain and two distinct binding sites with the p53 anti-oncoprotein and the p68 calcium-binding protein., PMID:1431101

Cell attachment activity of cementum proteins and mechanism of endotoxin inhibition., PMID:1833426

Repetitive P68-autoantigen specific epitopes recognized by human anti-(U1) small nuclear ribonucleoprotein autoantibodies., PMID:1723274

Identification of a putative RNA helicase in E.coli., PMID:2216714

RNA helicase activity associated with the human p68 protein., PMID:2471939

Datasheet

Document Download

Anti-DDX5 Antibody (R3E83).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-DDX5 Antibody (R3E83) [RHD26602]
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