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Anti-KRT5 Antibody (R3D35)

Catalog #:   RHC99505 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IF, IHC
Accession: P13647
Overview

Catalog No.

RHC99505

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IF: 1:200-1:1000, IHC: 1:200-1:1000

Target

Type-II keratin Kb5, Keratin, type II cytoskeletal 5, K5, 58 kDa cytokeratin, Cytokeratin-5, CK-5, KRT5, Keratin-5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P13647

Applications

IF, IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3D35

Data Image
  • Immunofluorescence
    Confocal Immunofluorescence analysis of methanol-fixed Eca-109 cells using Cytokeratin (Pan) mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A),normal hepatocyte (B), colon adenocacinoma, normal stomach tissue (D), showing cytoplasmic and membrane localization using CK mouse mAb with DAB staining.
References

Deciphering the Molecular Landscape of Urothelial Carcinoma: Immunohistochemistry-based Subtyping Using 4 Easily Available Antibodies for Cost-effective Stratification., PMID:40497570

Tear duct M cells exacerbate allergic conjunctivitis by facilitating germinal-center reactions., PMID:39900200

Identification of a Novel Subset of Human Airway Epithelial Basal Stem Cells., PMID:39337350

Candidate proteins interacting with cytoskeleton in cells from the basal airway epithelium in vitro., PMID:39139811

Feasibility and Impact of Immunohistochemistry-based Molecular Subtyping for Muscle-invasive Bladder Cancer in Patients Treated with Radiation-based Therapy., PMID:38020525

Keratin 5 in Lung Cancer Specimens: Comparison of Four Antibody Clones and KRT5 mRNA-ISH., PMID:37212698

Immunohistochemical Assays for Bladder Cancer Molecular Subtyping: Optimizing Parsimony and Performance of Lund Taxonomy Classifiers., PMID:35437049

Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia., PMID:34728556

Diagnostic and prognostic implications of a three-antibody molecular subtyping algorithm for non-muscle invasive bladder cancer., PMID:34697907

Molecular and immunohistochemical evaluation of BAP-1 antibody in bladder cancer and comparison with luminal-basal subtyping., PMID:33341088

CD44v3-Positive Intermediate Progenitor Cells Contribute to Airway Goblet Cell Hyperplasia., PMID:33264080

Efficacy of neoadjuvant atezolizumab treatment in patients with advanced urothelial bladder cancer according to the BASQ classification: a study protocol for an open-label, two-cohort, phase II trial., PMID:33060077

A direct immunohistochemistry (IHC) method improves the intraoperative diagnosis of breast papillary lesions including breast cancer., PMID:31926580

A direct immunohistochemistry (IHC) method improves the intraoperative diagnosis of breast papillary lesions including breast cancer., PMID:31465723

Molecular pathology of the luminal class of urothelial tumors., PMID:31232464

Mesenchymal stem cells differentiate into keratinocytes and express epidermal kallikreins: Towards an in vitro model of human epidermis., PMID:30891818

Searching for serum protein markers of equine squamous gastric disease using gel electrophoresis and mass spectrometry., PMID:30636330

Burnlike scars: A sign suggestive of KLHL24-related epidermolysis bullosa simplex., PMID:29574966

What we have lost: Mastitis resistance in Holstein Friesians and in a local cattle breed., PMID:29223308

Pathological expression of tissue factor confers promising antitumor response to a novel therapeutic antibody SC1 in triple negative breast cancer and pancreatic adenocarcinoma., PMID:28938620

Anthralin modulates the expression pattern of cytokeratins and antimicrobial peptides by psoriatic keratinocytes., PMID:28673488

Keratin 5 overexpression is associated with serous ovarian cancer recurrence and chemotherapy resistance., PMID:28147318

The expression patterns of aquaporin 9, vacuolar H+-ATPase, and cytokeratin 5 in the epididymis of the common vampire bat., PMID:27549752

Detection and quantification of epithelial progenitor cell populations in human healthy and IPF lungs., PMID:27423691

Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair., PMID:27322618

Isolating RNA from precursor and mature melanocytes from human vitiligo and normal skin using laser capture microdissection., PMID:27193292

Expressions of Thyroid Transcription Factor-1, Napsin A, p40, p63, CK5/6 and Desmocollin-3 in Non-Small Cell Lung Cancer, as Revealed by Imprint Cytology Using a Malinol-Based Cell-Transfer Technique., PMID:26696549

Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjögren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model., PMID:26179322

Cytokeratin 5/6 fingerprinting in HER2-positive tumors identifies a poor prognosis and trastuzumab-resistant basal-HER2 subtype of breast cancer., PMID:25742793

Aberrant differentiation of urothelial cells in patients with ureteropelvic junction obstruction., PMID:25337225

Epithelial basal cells are distinct from dendritic cells and macrophages in the mouse epididymis., PMID:24648397

T-lymphocytes are directly involved in the clinical expression of migratory circinate erythema in epidermolysis bullosa simplex patients., PMID:24104543

Constitutive β-catenin activation induces male-specific tumorigenesis in the bladder urothelium., PMID:23928991

Keratolysis exfoliativa (dyshidrosis lamellosa sicca): a distinct peeling entity., PMID:23039091

Verrucous carcinoma in epidermolysis bullosa simplex is possibly associated with a novel mutation in the keratin 5 gene., PMID:22639907

Tissue-sparing application of the newly proposed IASLC/ATS/ERS classification of adenocarcinoma of the lung shows practical diagnostic and prognostic impact., PMID:22586054

Mutations in KRT5 and KRT14 cause epidermolysis bullosa simplex in 75% of the patients., PMID:21375516

Caged fluorescent haptens reveal the generation of cryptic epitopes in allergic contact dermatitis., PMID:21228815

Clinical heterogeneity in recessive epidermolysis bullosa due to mutations in the keratin 14 gene, KRT14., PMID:18713255

Role of integrins, tetraspanins, and ADAM proteins during the development of apoptotic bodies by spermatogenic cells., PMID:16557522

Evaluation of 12 antibodies for distinguishing epithelioid mesothelioma from adenocarcinoma: identification of a three-antibody immunohistochemical panel with maximal sensitivity and specificity., PMID:16554731

The reactivity to CK5/6 antibody in tumor cells from non-small cell lung cancers shed into pleural effusions predicts survival., PMID:16465416

MDM2 protein expression is a negative prognostic marker in breast carcinoma., PMID:16258514

Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra., PMID:15867144

Clinical and immunohistochemical study of the effect of a cosmetic product in patients with asteatotic eczema., PMID:15847102

Cytokeratin 5/6 in normal human breast: lack of evidence for a stem cell phenotype., PMID:15376255

Expression of hair keratins in the adult nail unit: an immunohistochemical analysis of the onychogenesis in the proximal nail fold, matrix and nail bed., PMID:15327543

Different proliferative activity of the glandular and myoepithelial lineages in benign proliferative and early malignant breast diseases., PMID:15254552

Diagnostic utility of a p63/alpha-methyl-CoA-racemase (p504s) cocktail in atypical foci in the prostate., PMID:15205683

Improved 1-h rapid immunostaining method using intermittent microwave irradiation: practicability based on 5 years application in Toyama Medical and Pharmaceutical University Hospital., PMID:15167936

Datasheet

Document Download

Anti-KRT5 Antibody (R3D35).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-KRT5 Antibody (R3D35) [RHC99505]
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