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Anti-XRCC5/Ku80 Antibody (R3D09)

Catalog #:   RHC96101 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P13010
Overview

Catalog No.

RHC96101

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Lupus Ku autoantigen protein p86, XRCC5, Thyroid-lupus autoantigen, Ku86, ATP-dependent DNA helicase 2 subunit 2, CTC85, G22P2, DNA repair protein XRCC5, 86 kDa subunit of Ku antigen, TLAA, Nuclear factor IV, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit, CTCBF, Ku80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), X-ray repair cross-complementing protein 5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P13010

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3D09

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using XRCC5 mouse mAb (green) and negative control (purple).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using XRCC5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human tonsil tissues (left) and human colon cancer tissues (right) using XRCC5 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.
References

The DNA repair factor ku80 binds and activates the adhesion receptor ELTD1/ADGRL4., PMID:40245572

Nuclear expression of Ku70/80 is associated with CHEK2 germline mutations in breast cancer., PMID:37728466

Selenium Nanoparticles Can Influence the Immune Response Due to Interactions with Antibodies and Modulation of the Physiological State of Granulocytes., PMID:36559266

Characterization of Double-Strand Break Repair Protein Ku80 Location Within the Murine Retina., PMID:35737378

Chest pain in a mid-aged woman, not simply myopericarditis: a case report of anti-Ku positive polymyositis., PMID:34362296

Structural basis of long-range to short-range synaptic transition in NHEJ., PMID:33854234

Basic fibroblast growth factor promotes doxorubicin resistance in chondrosarcoma cells by affecting XRCC5 expression., PMID:31916307

Ku80 gene knockdown by the CRISPR/Cas9 technique affects the biological functions of human thyroid carcinoma cells., PMID:31578590

Blood Donation During Pregnancy Due to Anti-Ku Hemolytic Disease of the Fetus and Newborn., PMID:31089725

Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells., PMID:30897137

Camptocormia with trigeminal involvement revealing myositis with anti-Ku antibodies., PMID:29653283

Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination., PMID:28176781

Calcium influx-mediated translocation of m-calpain induces Ku80 cleavage and enhances the Ku80-related DNA repair pathway., PMID:27121057

The proteomic investigation reveals interaction of mdig protein with the machinery of DNA double-strand break repair., PMID:26293673

Influence of allelic Variations of hypoxia-related and DNA repair genes on patient outcome and toxicity in head and neck cancer treated with radiotherapy plus cetuximab., PMID:26245169

Genomic analysis of xCT-mediated regulatory network: Identification of novel targets against AIDS-associated lymphoma., PMID:25860939

Association Between Functional Polymorphisms of DNA Double-Strand Breaks in Repair Genes XRCC5, XRCC6 and XRCC7 with the Risk of Systemic Lupus Erythematosus in South East Iran., PMID:25756210

SMCHD1 accumulates at DNA damage sites and facilitates the repair of DNA double-strand breaks., PMID:24790221

Expression of Ku86 and presence of Ku86 antibody as biomarkers of hepatitis B virus related hepatocellular carcinoma., PMID:24271118

A subset of systemic sclerosis but not of systemic lupus erythematosus is defined by isolated anti-Ku autoantibodies., PMID:23910615

Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior., PMID:23868020

DNA ends alter the molecular composition and localization of Ku multicomponent complexes., PMID:22535209

DNA repair biomarker profiling of head and neck cancer: Ku80 expression predicts locoregional failure and death following radiotherapy., PMID:21349997

Ku86 is important for TrkA overexpression-induced breast cancer cell invasion., PMID:21137076

Use of phage display to isolate specific human monoclonal antibody fragments against a potential target for multiple myeloma., PMID:19652854

Ku86 represses lethal telomere deletion events in human somatic cells., PMID:19581589

Human DNA ligases I and III, but not ligase IV, are required for microhomology-mediated end joining of DNA double-strand breaks., PMID:18440984

Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features., PMID:18414048

Possible involvement of oxidative stress in fenofibrate-induced hepatocarcinogenesis in rats., PMID:18253720

Variation in DNA repair genes XRCC3, XRCC4, XRCC5 and susceptibility to myeloma., PMID:17901044

Wnt signaling controls radiosensitivity via cyclooxygenase-2-mediated Ku expression in head and neck cancer., PMID:17764107

Arsenite-induced alterations in Ku70-deficient cells: a model to study genotoxic effects., PMID:17479409

A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses., PMID:17396150

Electrophoretic mobility shift assays to study protein binding to damaged DNA., PMID:16673891

Association between autoantibodies to the Ku protein and DPB1*., PMID:15693000

Association of DNA-dependent protein kinase with hypoxia inducible factor-1 and its implication in resistance to anticancer drugs in hypoxic tumor cells., PMID:15272235

The Membrane-associated form of the DNA repair protein Ku is involved in cell adhesion to fibronectin., PMID:15019772

Biochemical evidence for Ku-independent backup pathways of NHEJ., PMID:12954774

DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97/VCP., PMID:12937274

Competition between the Rad50 complex and the Ku heterodimer reveals a role for Exo1 in processing double-strand breaks but not telomeres., PMID:12861005

Increased error-prone NHEJ activity in myeloid leukemias is associated with DNA damage at sites that recruit key nonhomologous end-joining proteins., PMID:12702565

Selective inhibition of class switching to IgG and IgE by recruitment of the HoxC4 and Oct-1 homeodomain proteins and Ku70/Ku86 to newly identified ATTT cis-elements., PMID:12672812

Novel localization of the DNA-PK complex in lipid rafts: a putative role in the signal transduction pathway of the ionizing radiation response., PMID:12672807

The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes., PMID:12663813

Interaction between Ku80 protein and a widely used antibody to adenomatous polyposis coli., PMID:12610503

Anti-Ku antibody-positive scleroderma-dermatomyositis overlap syndrome developing Graves' disease and immune thrombocytopenic purpura., PMID:12521216

Clinical significance of anti-Ku autoantibodies--a serologic marker of overlap syndrome?, PMID:12521194

Analysis of the DNA replication competence of the xrs-5 mutant cells defective in Ku86., PMID:12456721

Ku antigen, an origin-specific binding protein that associates with replication proteins, is required for mammalian DNA replication., PMID:12393188

Human Ku70/80 associates physically with telomerase through interaction with hTERT., PMID:12377759

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-XRCC5/Ku80 Antibody (R3D09) [RHC96101]
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