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Anti-RALA Antibody (R3C51)

Catalog #:   RHC87201 Specific References (44) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P11233
Overview

Catalog No.

RHC87201

Species reactivity

Human, Mouse, Rat, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:500, IHC: 1:100-1:500, WB: 1:500-1:2000

Target

Ras-related protein Ral-A, RAL, RALA

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P11233

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3C51

Data Image
  • Flow Cytometry
    Flow cytometric analysis of MCF-7 cells using RALA mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using RALA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using RALA mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using RALA mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse kidney using RALA mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse kidney using RALA mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat kidney using RALA mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using RALA mouse mAb against HepG2 (1), MCF-7 (2), A549 (3), K562 (4), Raji (5), MOLT4 (6), Hela (7), COS7 (8), and NIH3T3 (9) cell lysate.
  • Western blot
    Western blot analysis using RALA mouse mAb against Raw264.7(1)PC-12(2)NRK(3)C2C12(4)C6(5)L1210(6)F9(7)COS-7(8)CHO3D10(9) cell lysate.
References

Peptide delivery of a multivalent mRNA SARS-CoV-2 vaccine., PMID:37648082

Successful targeting in situ of an oncogenic nuclear antigen by hapten induced tumor associated autoantibodies (iTAA)., PMID:37336938

Awaken Immune Cells by Hapten Enhanced Intratumoral Chemotherapy with Penicillin Prolong Pancreatic Cancer Survival., PMID:37283801

Six autoantibodies as potential differential biomarkers of hepatocellular carcinoma vs. liver cirrhosis and chronic hepatitis: A prospective multi-institutional study., PMID:36238856

Presence of serum RalA and serum p53 autoantibodies in 1833 patients with various types of cancers., PMID:34632560

Development of recombinant biomimetic nano-carrier for targeted gene transfer to HER3 positive breast cancer., PMID:34455002

Seroprevalence of Toxocara spp. in children (3-13 years old) in Zahedan, Southeast of Iran., PMID:34295044

Absolute Quantitation of GTPase Protein Abundance., PMID:33977471

Multipanel assay of 17 tumor-associated antibodies for serological detection of stage 0/I breast cancer., PMID:33605508

Possible predictive significance of serum RalA autoantibodies on relapse-free survival in patients with colorectal cancer., PMID:33363728

The immunophilin Zonda controls regulated exocytosis in endocrine and exocrine tissues., PMID:33336828

Prevalence of autoantibodies against Ras-like GTPases, RalA, in patients with gastric cancer., PMID:32765875

Six autoantibodies as potential serum biomarkers of hepatocellular carcinoma: A prospective multicenter study., PMID:32574375

Development of a ZHER3-Affibody-Targeted Nano-Vector for Gene Delivery to HER3-Overexpressed Breast Cancer Cells., PMID:31531954

Using a panel of multiple tumor-associated antigens to enhance the autoantibody detection in the immunodiagnosis of ovarian cancer., PMID:30484895

Multi-panel assay of serum autoantibodies in colorectal cancer., PMID:29691673

Evaluation of serum autoantibodies against tumor-associated antigens as biomarkers in lung cancer., PMID:29022480

Serum autoantibodies against a panel of 15 tumor-associated antigens in the detection of ovarian cancer., PMID:28618923

Probing Functional Changes in Exocyst Configuration with Monoclonal Antibodies., PMID:27376061

Evaluation and characterization of anti-RalA autoantibody as a potential serum biomarker in human prostate cancer., PMID:27286458

Genes involved in pericyte-driven tumor maturation predict treatment benefit of first-line FOLFIRI plus bevacizumab in patients with metastatic colorectal cancer., PMID:25069475

Preferential autoimmune response in prostate cancer to cyclin B1 in a panel of tumor-associated antigens., PMID:24860838

Mini-array of multiple tumor-associated antigens (TAAs) in the immunodiagnosis of esophageal cancer., PMID:24761876

Response to MLN8237 in pancreatic cancer is not dependent on RalA phosphorylation., PMID:24222664

Ral GTPases regulate cell-mediated cytotoxicity in NK cells., PMID:21810610

Analyzing the function of small GTPases by microinjection of plasmids into polarized epithelial cells., PMID:21673637

Phosphorylation of RalB is important for bladder cancer cell growth and metastasis., PMID:20940393

Immunogenicity of Ra1A and its tissue-specific expression in hepatocellular carcinoma., PMID:19822090

Autoantibodies to tumor-associated antigens combined with abnormal alpha-fetoprotein enhance immunodiagnosis of hepatocellular carcinoma., PMID:19683863

Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein., PMID:19299511

Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct., PMID:18596208

R-Ras regulates exocytosis by Rgl2/Rlf-mediated activation of RalA on endosomes., PMID:17344481

RalA-exocyst-dependent recycling endosome trafficking is required for the completion of cytokinesis., PMID:17028198

Phosphorylation of 46-kDa protein of synaptic vesicle membranes is stimulated by GTP and Ca2+/calmodulin., PMID:12526085

The GDP exchange factor AND-34 is expressed in B cells, associates with HEF1, and activates Cdc42., PMID:12517963

Role for phospholipase D in receptor-mediated endocytosis., PMID:11134345

Small GTP-binding protein RalA associates with Weibel-Palade bodies in endothelial cells., PMID:10494784

Immunodetection of ralA and ralB GTP-binding proteins in various rat tissues and platelets., PMID:9543348

Generation of antibodies specific for the RalA and RalB GTP-binding proteins and determination of their concentration and distribution in human platelets., PMID:8972729

Involvement of Ral GTPase in v-Src-induced phospholipase D activation., PMID:7477381

Molecular cloning of cDNAs encoding a guanine-nucleotide-releasing factor for Ras p21., PMID:1379346

Identification of some of the brain Gn27 as the ral gene product. Comparison between the brain and platelet Gn-proteins., PMID:1544424

Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA., PMID:1903399

Identification of multiple ral gene products in human platelets that account for some but not all of the platelet Gn-proteins., PMID:2105235

Datasheet

Document Download

Anti-RALA Antibody (R3C51).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-RALA Antibody (R3C51) [RHC87201]
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