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Anti-HSPA1A Antibody (R3B95)

Catalog #:   RHC69304 Specific References (29) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IHC, IP, WB
Accession: P0DMV8
Overview

Catalog No.

RHC69304

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:100-1:200, IP: 1:100-1:200, WB: 1:500-1:1000

Target

HSP70.1, HSP70-1, HSPA1, HSPA1A, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, HSP72, HSX70

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P0DMV8

Applications

IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3B95

Data Image
  • Immunohistochemical
    IHC-P analysis of testis tissue by HSPA1A antibody (RHC69304). IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue;Result: Cells in seminiferous ducts are positively stained at the cytoplasm.
  • Immunoprecipitation
    Lane 1: A549 lysate ; Lane 2: HSPA1A immunoprecipitated from A549 lysate by RHC69304;Lane 3: The same as Lane 2 but use IgG isotype control antibody ;Result: RHC69304 can immunoprecipitate HSPA1A;
  • Western blot
    Western blot analysis using HSPA1A mouse mAb against A549 lysate
References

Single-cell transcriptomic analysis reveals distinct plasma cell populations in chronic thromboembolic pulmonary hypertension., PMID:39965671

Comprehensive multiplexed autoantibody profiling of patients with advanced urothelial cancer., PMID:38309723

Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection., PMID:37434783

Integrative system biology and mathematical modeling of genetic networks identifies shared biomarkers for obesity and diabetes., PMID:35240786

FHL-1 interacts with human RPE cells through the α5β1 integrin and confers protection against oxidative stress., PMID:34239032

Time- and Dose-Dependent Effects of Ionizing Irradiation on the Membrane Expression of Hsp70 on Glioma Cells., PMID:32276468

Dual-platform affinity proteomics identifies links between the recurrence of ovarian carcinoma and proteins released into the tumor microenvironment., PMID:31588238

HSP70 Facilitates Memory Consolidation of Fear Conditioning through MAPK Pathway in the Hippocampus., PMID:29374537

Overexpression of cytosolic, plasma membrane bound and extracellular heat shock protein 70 (Hsp70) in primary glioblastomas., PMID:28849427

Molecular markers of systemic autoimmune disorders: the expression of MHC-located HSP70 genes is significantly associated with autoimmunity development., PMID:28032847

Systematic protein-protein interaction and pathway analyses in the idiopathic inflammatory myopathies., PMID:27388770

[Role of heat shock proteins in the cardioprotection of regular moderate alcohol consumption]., PMID:26902796

Hsp70 (HSPA1) Lysine Methylation Status as a Potential Prognostic Factor in Metastatic High-Grade Serous Carcinoma., PMID:26448330

Early inflammatory response in polytraumatized patients: Cytokines and heat shock proteins. A pilot study., PMID:26068807

Reactive γ-ketoaldehydes promote protein misfolding and preamyloid oligomer formation in rapidly-activated atrial cells., PMID:25463275

Transcriptome profiling and genome-wide DNA binding define the differential role of fenretinide and all-trans RA in regulating the death and survival of human hepatocellular carcinoma Huh7 cells., PMID:23396089

Amphibian oocytes release heat shock protein A during spawning: evidence for a role in fertilization., PMID:22623622

The effects of fever on hormone ghrelins, immunoglobulins, and heat shock protein 70 expression after swine flu vaccinations., PMID:22477150

Cellular protein quality control and the evolution of aggregates in spinocerebellar ataxia type 3 (SCA3)., PMID:21916928

Measuring Hsp72 (HSPA1A) by indirect sandwich ELISA., PMID:21898234

Variants of HSPA1A in combination with plasma Hsp70 and anti-Hsp70 antibody levels associated with higher risk of acute coronary syndrome., PMID:21849784

Distinguishing integral and receptor-bound heat shock protein 70 (Hsp70) on the cell surface by Hsp70-specific antibodies., PMID:21165727

Comment to: Humanization of a mouse monoclonal antibody directed against a cell surface-exposed epitope of membrane-associated heat shock protein 70 (Hsp70)., PMID:20668964

Extracellular heat shock protein 70 (HSPA1A) and classical vascular risk factors in a general population., PMID:20490736

HspBP1 levels are elevated in breast tumor tissue and inversely related to tumor aggressiveness., PMID:18987994

The equatorial subsegment in mammalian spermatozoa is enriched in tyrosine phosphorylated proteins., PMID:18448843

Association analysis of heat shock protein 70 gene polymorphisms in schizophrenia., PMID:18299791

Comparison of anti-heat shock protein 70 (anti-hsp70) and anti-68-kDa inner ear protein in the sera of patients with Meniere's disease., PMID:9023247

Pharmacological induction of heat shock protein 68 synthesis in cultured rat astrocytes., PMID:8530397

Datasheet

Document Download

Anti-HSPA1A Antibody (R3B95).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-HSPA1A Antibody (R3B95) [RHC69304]
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