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Anti-ANXA5 Antibody (R3B38)

Catalog #:   RHC36302 Specific References (47) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, WB
Accession: P08758
Overview

Catalog No.

RHC36302

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

Endonexin II, ENX2, Placental anticoagulant protein 4, PP4, CBP-I, ANXA5, Anchorin CII, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha, Calphobindin I, ANX5, Placental anticoagulant protein I, Annexin V, Annexin-5, Lipocortin V, Annexin A5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P08758

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3B38

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using ANXA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Western blot
    Western blot analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate.
References

S-hydroxychloroquine prevents the antiphospholipid thrombogenic complexes for antiphospholipid syndrome treatment., PMID:40120554

Anti-fibroblast and anti-endothelial cell autoantibodies in pulmonary arterial hypertension (PAH) in patients with connective tissue diseases (CTD)., PMID:39918970

Heterologous expression, purification and generation of specific antibodies for Annexin A8., PMID:39674528

Association of Anti-Annexin A5 Antibody With Pregnancy Outcomes: A Cohort Study: Anti-annexin A5 antibody with pregnancy outcomes., PMID:39381915

Annexin A5 is a novel prognostic biomarker in oral squamous cell carcinoma., PMID:38945807

Coxsackievirus A6 Infection Causes Neurogenic Pathogenesis in a Neonatal Murine Model., PMID:36851724

Long-circulating XTEN864-annexin A5 fusion protein for phosphatidylserine-related therapeutic applications., PMID:34405304

The ABCA1-efferocytosis axis: A new strategy to protect against atherosclerosis., PMID:33741356

Low prevalence of antiannexin A5 antibodies in unprovoked venous thrombosis., PMID:33586909

Overexpression of annexin A5 might guide the gemtuzumab ozogamicin treatment choice in patients with pediatric acute myeloid leukemia., PMID:32636939

Annexin A5 as an immune checkpoint inhibitor and tumor-homing molecule for cancer treatment., PMID:32111835

Corneal Denervation Causes Epithelial Apoptosis Through Inhibiting NAD+ Biosynthesis., PMID:31415077

Induction of DDIT4 Impairs Autophagy Through Oxidative Stress in Dry Eye., PMID:31266058

Absence of Distinct Immunohistochemical Distribution of Annexin A5, C3b, C4d, and C5b-9 in Placentas From Patients With Antiphospholipid Antibodies, Preeclampsia, and Systemic Lupus Erythematosus., PMID:30922166

Antiphospholipid Antibodies in a General Obstetric Population: Clinical Impact on Pregnancy Outcome and Relationship with the M2 Haplotype in the Annexin A5 (ANXA5) Gene., PMID:30304747

Anti-CD73 and anti-OX40 immunotherapy coupled with a novel biocompatible enzyme prodrug system for the treatment of recurrent, metastatic ovarian cancer., PMID:29574275

The effect of hydroxychloroquine on haemostasis, complement, inflammation and angiogenesis in patients with antiphospholipid antibodies., PMID:29045759

Prediction of Antiphospholipid syndrome using Annexin A5 competition assay in patients with SLE., PMID:27704312

Circulating Autoantibodies in Age-Related Macular Degeneration Recognize Human Macular Tissue Antigens Implicated in Autophagy, Immunomodulation, and Protection from Oxidative Stress and Apoptosis., PMID:26717306

Examining the prevalence of non-criteria anti-phospholipid antibodies in patients with anti-phospholipid syndrome: a systematic review., PMID:26152548

XTEN-annexin A5: XTEN allows complete expression of long-circulating protein-based imaging probes as recombinant alternative to PEGylation., PMID:24549285

[Annexins and recurrent pregnancy loss]., PMID:24152411

The haplotype M2 of the ANXA5 gene is not associated with antitrophoblast antibodies., PMID:23529182

The annexin A5 protective shield model revisited: inherited carriage of the M2/ANXA5 haplotype in placenta as a predisposing factor for the development of obstetric antiphospholipid antibodies., PMID:22635237

Surface plasmon resonance shows a gender difference in circulating annexin A5 in human., PMID:22483902

Annexin A5 binds to lipopolysaccharide and reduces its endotoxin activity., PMID:22415004

Circulating cell-derived microparticles in severe preeclampsia and in fetal growth restriction., PMID:21992597

Resistance to annexin A5 anticoagulant activity in women with histories for obstetric antiphospholipid syndrome., PMID:21784397

Prior exposure to oxidized low-density lipoprotein limits apoptosis in subsequent generations of endothelial cells by altering promoter methylation., PMID:21602467

Site-specific 68Ga-labeled Annexin A5 as a PET imaging agent for apoptosis., PMID:21492787

Multi-modal proteomic analysis of retinal protein expression alterations in a rat model of diabetic retinopathy., PMID:21249158

The annexin A5-mediated pathogenic mechanism in the antiphospholipid syndrome: role in pregnancy losses and thrombosis., PMID:20353989

Effects of anti-cardiolipin antibodies and IVIg on annexin A5 binding to endothelial cells: implications for cardiovascular disease., PMID:20132075

Anti-annexin A5 antibodies in women with spontaneous pregnancy loss., PMID:20022614

Hydroxychloroquine protects the annexin A5 anticoagulant shield from disruption by antiphospholipid antibodies: evidence for a novel effect for an old antimalarial drug., PMID:19965621

Both early and late apoptotic blebs are taken up by DC and induce IL-6 production., PMID:19811290

Gold fluorescent annexin A5 as a novel apoptosis detection tool., PMID:19437539

Resistance to annexin A5 anticoagulant activity: a thrombogenic mechanism for the antiphospholipid syndrome., PMID:18827057

Oxidized LDL, anti-oxidized LDL and anti-annexin A5 antibodies in primary antiphospholipid syndrome., PMID:18630740

Annexin A5 as a novel player in prevention of atherothrombosis in SLE and in the general population., PMID:17893975

Correlation between antiphospholipid antibodies that recognize domain I of beta2-glycoprotein I and a reduction in the anticoagulant activity of annexin A5., PMID:17053060

Antithrombotic action of annexin V proved as efficient as direct inhibition of tissue factor or thrombin., PMID:16919046

Annexin A5 binding to giant phospholipid vesicles is differentially affected by anti-beta2-glycoprotein I and anti-annexin A5 antibodies., PMID:16820381

Analysis of circulating annexin A5 parameters during pregnancy: absence of differences between women with recurrent spontaneous pregnancy losses and controls., PMID:16681990

Annexin A5 in cardiovascular disease and systemic lupus erythematosus., PMID:16325495

Antibodies against annexin A5: detection pitfalls and clinical associations., PMID:16278147

Cytokine modulation of liver annexin 1 expression during experimental endotoxemia., PMID:11583971

Datasheet

Document Download

Anti-ANXA5 Antibody (R3B38).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-ANXA5 Antibody (R3B38) [RHC36302]
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