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Anti-AIFM1/AIF Antibody (R2W44)

Catalog #:   RHB76001 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA, FCM, IF, IHC, WB
Accession: O95831
Overview

Catalog No.

RHB76001

Species reactivity

Human, Mouse, Rat, Monkey

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

AIFM1, AIF, Programmed cell death protein 8, PDCD8, Apoptosis-inducing factor 1, mitochondrial

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O95831

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R2W44

Data Image
  • Flow Cytometry
    Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of RSC-96 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of NIH3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AIF mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using AIF mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse liver(A) Mouse kidney(B) using AIF mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat liver(A) Rat kidney(B) using AIF mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.
  • Western blot
    Western blot analysis using AIF mouse mAb against CHO3D10(1),COS7(2),F9(3),L1210(4),C6(5),C2C12(6),NIH/3T3(7),Raw264.7(8),PC-12(9) cell lysate.
References

Newly synthesized AIFM1 determines the hypersensitivity of T lymphocytes to STING activation-induced cell apoptosis., PMID:37000625

Anti-neuronal antibodies against brainstem antigens are associated with COVID-19., PMID:35963198

CRKL, AIFM3, AIF, BCL2, and UBASH3A during Human Kidney Development., PMID:34502088

Phosphorylation-Dependent Interactome of Ryanodine Receptor Type 2 in the Heart., PMID:34200203

CREG1 promotes lysosomal biogenesis and function., PMID:33966596

Concurrent mutations associated with trastuzumab-resistance revealed by single cell sequencing., PMID:33905021

Live-cell imaging to measure BAX recruitment kinetics to mitochondria during apoptosis., PMID:28880942

Retinal Inhibition of CCR3 Induces Retinal Cell Death in a Murine Model of Choroidal Neovascularization., PMID:27309355

CD40 ligand induces RIP1-dependent, necroptosis-like cell death in low-grade serous but not serous borderline ovarian tumor cells., PMID:26313915

[Protective action of glutamate antibodies on increased expression of genes of programmed death of rat brain cells induced by injection of a β-amyloid fragment (25-35)]., PMID:25735164

Major differences between tumor and normal human cell fates after exposure to chemotherapeutic monofunctional alkylator., PMID:24019948

The neurotoxicity of hallucinogenic amphetamines in primary cultures of hippocampal neurons., PMID:22983118

Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype., PMID:22076146

The radiolabeling of proteins by the [18F]AlF method., PMID:21890371

The synthesized novel fluorinated compound (LJJ-10) induces death receptor- and mitochondria-dependent apoptotic cell death in the human osteogenic sarcoma U-2 OS cells., PMID:21514013

Proteomic analysis of childhood de novo acute myeloid leukemia and myelodysplastic syndrome/AML: correlation to molecular and cytogenetic analyses., PMID:20711619

Sirt1 involvement in rd10 mouse retinal degeneration., PMID:19407027

alpha-Skeletal muscle actin nemaline myopathy mutants cause cell death in cultured muscle cells., PMID:19393268

Ascorbate (vitamin C) induces cell death through the apoptosis-inducing factor in human breast cancer cells., PMID:17786340

[Cloning and expression of recombinant mitochondrial Delta1-120 apoptosis-inducing factor and its enhancive effect on apoptosis of hepatocellular carcinoma cell line SMMC-7721]., PMID:17672939

CD44 ligation induces caspase-independent cell death via a novel calpain/AIF pathway in human erythroleukemia cells., PMID:16636662

Apoptosis-inducing factor of a cytotoxic T cell line: involvement of a secretory phospholipase A2., PMID:16609916

Metalloporphyrin-based superoxide dismutase mimic attenuates the nuclear translocation of apoptosis-inducing factor and the subsequent DNA fragmentation after permanent focal cerebral ischemia in mice., PMID:16269636

Selective proapoptotic activity of a secreted recombinant antibody/AIF fusion protein in carcinomas overexpressing HER2., PMID:16267568

Early nuclear translocation of endonuclease G and subsequent DNA fragmentation after transient focal cerebral ischemia in mice., PMID:15979239

Oligomeric Bax is a component of the putative cytochrome c release channel MAC, mitochondrial apoptosis-induced channel., PMID:15772159

Molecular cloning and characterization of a human AIF-like gene with ability to induce apoptosis., PMID:15764604

Beta-carbolines induce apoptosis in cultured cerebellar granule neurons via the mitochondrial pathway., PMID:15617732

Apoptosis-inducing factor substitutes for caspase executioners in NMDA-triggered excitotoxic neuronal death., PMID:15574746

Caspase-independent component of retinal ganglion cell death, in vitro., PMID:15505055

Survival of retinal pigment epithelium after exposure to prolonged oxidative injury: a detailed gene expression and cellular analysis., PMID:15452088

Anti-apoptotic mechanism and reduced expression of phospholipase D in spontaneous and Fas-stimulated apoptosis of human neutrophils., PMID:15368292

Apoptosis-inducing factor determines the chemoresistance of non-small-cell lung carcinomas., PMID:15286713

Cross-linking of P-selectin glycoprotein ligand-1 induces death of activated T cells., PMID:15198951

Mitochondrial permeability transition induced by reactive oxygen species is independent of cholesterol-regulated membrane fluidity., PMID:14987999

Activated protein C prevents neuronal apoptosis via protease activated receptors 1 and 3., PMID:14980205

Mediating of caspase-independent apoptosis by cadmium through the mitochondria-ROS pathway in MRC-5 fibroblasts., PMID:14743397

TCR-independent and caspase-independent apoptosis of murine thymocytes by CD24 cross-linking., PMID:14707049

Mitochondrial dysfunction in CD47-mediated caspase-independent cell death: ROS production in the absence of cytochrome c and AIF release., PMID:14585540

Apoptosis induction by interleukin-2-activated cytotoxic lymphocytes in a squamous cell carcinoma cell line and Daudi cells - involvement of reactive oxygen species-dependent cytochrome c and reactive oxygen species-independent apoptosis-inducing factors., PMID:14511235

Mitochondria control of cell death induced by anti-HLA-DR antibodies., PMID:12835725

Clearance of apoptotic photoreceptors: elimination of apoptotic debris into the subretinal space and macrophage-mediated phagocytosis via phosphatidylserine receptor and integrin alphavbeta3., PMID:12759244

Human colon cancer cells differ in their sensitivity to curcumin-induced apoptosis and heat shock protects them by inhibiting the release of apoptosis-inducing factor and caspases., PMID:12633846

Apoptosis-inducing protein derived from hepatocyte selectively induces apoptosis in lymphocytes., PMID:12519310

Role of caspases and apoptosis-inducing factor (AIF) in cladribine-induced apoptosis of B cell chronic lymphocytic leukemia., PMID:12357364

Apoptosis-inducing factor is involved in the regulation of caspase-independent neuronal cell death., PMID:12147675

Mediation of poly(ADP-ribose) polymerase-1-dependent cell death by apoptosis-inducing factor., PMID:12114629

Apoptosis-inducing factor (AIF): a novel caspase-independent death effector released from mitochondria., PMID:12022952

Productive HIV-1 infection of primary CD4+ T cells induces mitochondrial membrane permeabilization leading to a caspase-independent cell death., PMID:11689551

On the evolutionary conservation of the cell death pathway: mitochondrial release of an apoptosis-inducing factor during Dictyostelium discoideum cell death., PMID:11598188

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-AIFM1/AIF Antibody (R2W44) [RHB76001]
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