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Anti-PDE5A Antibody (R2W03)

Catalog #:   RHB40701 Specific References (27) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: IHC, IP, WB
Accession: O76074
Overview

Catalog No.

RHB40701

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

IHC: 1:100-1:200, IP: 1:100-1:200, WB: 1:500-1:1000

Target

CGB-PDE, cGMP-specific 3',5'-cyclic phosphodiesterase, PDE5A, PDE5, cGMP-binding cGMP-specific phosphodiesterase

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O76074

Applications

IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R2W03

Data Image
  • Immunohistochemical
    IHC-P analysis of colon tissue by PDE5A antibody (RHB40701). IHC-P was performed using sections of the formalin-fixed paraffin-embedded colon tissue;Result: Glandular cells are positively stained at the cytoplasm.
  • Immunoprecipitation
    Lane 1: HEK-293 lysate; Lane 2: PDE5A immunoprecipitated from HEK-293 lysate by RHB40701;Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: RHB40701 can immunoprecipitate PDE5A;
  • Western blot
    Western blot analysis using PDE5A mouse mAb against HEK-293 lysate and MCF7 lysate
References

Homogeneous single-label cGMP detection platform for the functional study of nitric oxide-sensitive (soluble) guanylyl cyclases and cGMP-specific phosphodiesterases., PMID:33060787

The role of PDE5a in oocyte maturation of zebrafish., PMID:31654676

Expression and distribution of phosphodiesterase isoenzymes in the human male urethra., PMID:25704994

Alterations in the interactome of serine/threonine protein phosphatase type-1 in atrial fibrillation patients., PMID:25593058

Defining the phosphodiesterase superfamily members in rat brain microvessels., PMID:22860158

Expression and distribution of phosphodiesterase isoenzymes in the human seminal vesicles., PMID:21810184

[Phoshodiesterases. The ''lords of the ring'' meet IgA nephropathy]., PMID:21132633

Neuronal nitric oxide synthase supports Renin release during sodium restriction through inhibition of phosphodiesterase 3., PMID:20651700

Immunohistochemical description of cyclic nucleotide phosphodiesterase (PDE) isoenzymes in the human labia minora., PMID:17498099

Immunohistochemical staining as a potential method for the identification of vaginal epithelial cells in forensic casework., PMID:17018095

Expression of cAMP and cGMP-phosphodiesterase isoenzymes 3, 4, and 5 in the human clitoris: immunohistochemical and molecular biology study., PMID:16635522

Immunohistochemical localisation of PDE5 in Leydig and myoid cells of prepuberal and adult rat testis., PMID:16163485

3',5'-cyclic nucleotide phosphodiesterase 11A: localization in human tissues., PMID:15800651

Phosphodiesterase 5 enzyme and its inhibitors: update on pharmacological and therapeutical aspects., PMID:15672122

Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa., PMID:14764637

The presence and function of phosphodiesterase type 5 in the rat myometrium., PMID:14749671

Individual cerebellar Purkinje cells express different cGMP phosphodiesterases (PDEs): in vivo phosphorylation of cGMP-specific PDE (PDE5) as an indicator of cGMP-dependent protein kinase (PKG) activation., PMID:12878685

Nitric oxide enhances angiogenesis via the synthesis of vascular endothelial growth factor and cGMP after stroke in the rat., PMID:12595343

PDE5 is converted to an activated state upon cGMP binding to the GAF A domain., PMID:12554648

CP248, a derivative of exisulind, causes growth inhibition, mitotic arrest, and abnormalities in microtubule polymerization in glioma cells., PMID:12477052

Type 5 phosphodiesterase expression in the human vagina., PMID:12100961

Regulation of cGMP-specific phosphodiesterase (PDE5) phosphorylation in smooth muscle cells., PMID:11723116

Expression of cGMP-binding cGMP-specific phosphodiesterase (PDE5) in mouse tissues and cell lines using an antibody against the enzyme amino-terminal domain., PMID:11389965

Cross-regulation of intracellular cGMP and cAMP in cultured human corpus cavernosum smooth muscle cells., PMID:11152621

Immunohistochemical localization of cGMP-binding cGMP-specific phosphodiesterase (PDE5) in rat tissues., PMID:10769052

Expression and regulation of the cGMP-binding, cGMP-specific phosphodiesterase (PDE5) in human colonic epithelial cells: role in the induction of cellular refractoriness to the heat-stable enterotoxin peptide., PMID:10679826

A cyclic nucleotide PDE5 inhibitor corrects defective mucin secretion in submandibular cells containing antibody directed against the cystic fibrosis transmembrane conductance regulator protein., PMID:10611481

Datasheet

Document Download

Anti-PDE5A Antibody (R2W03).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-PDE5A Antibody (R2W03) [RHB40701]
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