Catalog No.
RHH27401
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IP: 1:20-1:50, WB: 1:1000-1:2000
Target
Retinoid isomerohydrolase, Retinol isomerase, Retinal pigment epithelium-specific 65 kDa protein, RPE65, Lutein isomerase, Meso-zeaxanthin isomerase, All-trans-retinyl-palmitate hydrolase
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q16518
Applications
IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2G92
Genetic mutation in HSF4 is associated with retinal degeneration in mice., PMID:40023307
Blastocyst-like Structures in the Peripheral Retina of Young Adult Beagles., PMID:38892233
Hyperreflective Foci, Optical Coherence Tomography Progression Indicators in Age-Related Macular Degeneration, Include Transdifferentiated Retinal Pigment Epithelium., PMID:34448806
Effect of Long-term Anti-VEGF Treatment on Viability and Function of RPE Cells., PMID:33998943
Immune responses to retinal gene therapy using adeno-associated viral vectors - Implications for treatment success and safety., PMID:33069860
Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration., PMID:33027920
Palmitoylation of Metazoan Carotenoid Oxygenases., PMID:32331396
Safety of Same-Eye Subretinal Sequential Readministration of AAV2-hRPE65v2 in Non-human Primates., PMID:31660416
Utility of In Vitro Mutagenesis of RPE65 Protein for Verification of Mutational Pathogenicity Before Gene Therapy., PMID:31580392
Subretinal Transplantation of Human Central Nervous System Stem Cells Stimulates Controlled Proliferation of Endogenous Retinal Pigment Epithelium., PMID:31245172
Effects of Ranibizumab, Bevacizumab, and Aflibercept on Senescent Retinal Pigment Epithelial Cells., PMID:30091312
Deficiency of type 2 iodothyronine deiodinase reduces necroptosis activity and oxidative stress responses in retinas of Leber congenital amaurosis model mice., PMID:29874126
Ocular Histopathology and Immunohistochemical Analysis in the Oldest Known Individual with Autosomal Dominant Vitreoretinochoroidopathy., PMID:29774302
Antigen-specificity of antiretinal antibodies in patients with noninfectious uveitis., PMID:28985805
The effects of platelet gel on cultured human retinal pigment epithelial (hRPE) cells., PMID:28632489
Association of Circulating Markers With Outcome Parameters in the Bevacizumab and Ranibizumab in Diabetic Macular Edema Trial., PMID:27842163
Tyrosinase-Cre-Mediated Deletion of the Autophagy Gene Atg7 Leads to Accumulation of the RPE65 Variant M450 in the Retinal Pigment Epithelium of C57BL/6 Mice., PMID:27537685
Time-Dependent Changes of Cell Proliferation After Laser Photocoagulation in Mouse Chorioretinal Tissue., PMID:25813995
Histopathology of the Retina from a Three Year-Old Suspected to Have Joubert Syndrome., PMID:27747301
Protection of cone photoreceptor M-opsin degradation with 9-cis-β-carotene-rich alga Dunaliella bardawil in Rpe65(-/-) mouse retinal explant culture., PMID:25006880
Update on current and future novel therapies for dry age-related macular degeneration., PMID:23971874
Subretinal implantation of retinal pigment epithelial cells derived from human embryonic stem cells: improved survival when implanted as a monolayer., PMID:23833067
Bioactive substrates for human retinal pigment epithelial cell growth from elastin-like recombinamers., PMID:23554132
Histology and immunochemistry evaluation of autologous translocation of retinal pigment epithelium-choroid graft in porcine eyes., PMID:23256869
Differential proteomics and functional research following gene therapy in a mouse model of Leber congenital amaurosis., PMID:22953002
The human rhodopsin kinase promoter in an AAV5 vector confers rod- and cone-specific expression in the primate retina., PMID:22845794
Early apoptosis of rod photoreceptors in Rpe65(-/-) mice is associated with the upregulated expression of lysosomal-mediated autophagic genes., PMID:22227450
Histopathology and functional correlations in a patient with a mutation in RPE65, the gene for retinol isomerase., PMID:21931134
Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19., PMID:21743014
An alternative isomerohydrolase in the retinal Müller cells of a cone-dominant species., PMID:21676174
The Usher 1B protein, MYO7A, is required for normal localization and function of the visual retinoid cycle enzyme, RPE65., PMID:21493626
Elastin-like recombinamers as substrates for retinal pigment epithelial cell growth., PMID:21442725
Gene therapy rescues cone structure and function in the 3-month-old rd12 mouse: a model for midcourse RPE65 leber congenital amaurosis., PMID:21169527
Gene therapy in the second eye of RPE65-deficient dogs improves retinal function., PMID:20703309
Altered expression of retinal molecular markers in the canine RPE65 model of Leber congenital amaurosis., PMID:20671290
Quantification of retinal pigment epithelial phenotypic variation using laser scanning cytometry., PMID:20606706
Effective and sustained delivery of hydrophobic retinoids to photoreceptors., PMID:20574023
Safety and efficacy of subretinal readministration of a viral vector in large animals to treat congenital blindness., PMID:20374996
Importance of membrane structural integrity for RPE65 retinoid isomerization activity., PMID:20100834
A battery of cell- and structure-specific markers for the adult porcine retina., PMID:20086234
Norrin promotes vascular regrowth after oxygen-induced retinal vessel loss and suppresses retinopathy in mice., PMID:20053900
Gene therapy for Leber's congenital amaurosis is safe and effective through 1.5 years after vector administration., PMID:19953081
In vivo analysis of cone survival in mice., PMID:19737879
Human RPE65 gene therapy for Leber congenital amaurosis: persistence of early visual improvements and safety at 1 year., PMID:19583479
The 11-cis-retinol dehydrogenase activity of RDH10 and its interaction with visual cycle proteins., PMID:19458327
Isolation and characterization of a spontaneously immortalized bovine retinal pigmented epithelial cell line., PMID:19413901
Cone outer segment morphology and cone function in the Rpe65-/- Nrl-/- mouse retina are amenable to retinoid replacement., PMID:19407011
Subretinal delivery of adeno-associated virus serotype 2 results in minimal immune responses that allow repeat vector administration in immunocompetent mice., PMID:19340848
Bacillus cereus-induced permeability of the blood-ocular barrier during experimental endophthalmitis., PMID:19264886
Gene therapy following subretinal AAV5 vector delivery is not affected by a previous intravitreal AAV5 vector administration in the partner eye., PMID:19190735