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Anti-SAFB Antibody (R2G39)

Catalog #:   RHH09601 Specific References (30) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, IHC, WB
Accession: Q15424
Overview

Catalog No.

RHH09601

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IHC: 1:50-1:100, WB: 1:500-1:1000

Target

Scaffold attachment factor B1, HET, HSP27 ERE-TATA-binding protein, SAF-B, HSP27 estrogen response element-TATA box-binding protein, SAFB, SAF-B1, SAFB1, HAP

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q15424

Applications

IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R2G39

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of SAFB1 (green) in 293 using SAFB1 antibody,and DAPI(blue).
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SAFB antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot
    Western blot analysis of SAFB in K562, 3T3, Hela lysates using SAFB antibody.
References

A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB., PMID:37283223

A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB., PMID:37066147

Stability of Anti-HLA Sensitization Profiles in Highly Sensitized Kidney Transplantation Candidates: Toward a Rational Serological Testing Strategy., PMID:34028385

Monoclonal secondary reagents do not outperform polyclonal secondary reagents for detection of anti-HLA IgG using single antigen flow beads assays., PMID:32530111

Measuring anti-HLA antibody active concentration and affinity by surface plasmon resonance: Comparison with the luminex single antigen flow beads and T-cell flow cytometry crossmatch results., PMID:30776727

The disappointing contribution of anti-human leukocyte antigen donor-specific antibodies characteristics for predicting allograft loss., PMID:29672702

Prevalence, distribution and amplitude of the complement interference phenomenon in single antigen flow beads assays., PMID:29604172

Autoantibody to scaffold attachment factor B (SAFB): A novel connective tissue disease-related autoantibody associated with interstitial lung disease., PMID:27682649

Reassessment of T Lymphocytes Crossmatches Results Prediction With Luminex Class I Single Antigen Flow Beads Assay., PMID:27336398

Evaluation of the iBeads assay as a tool for identifying class I HLA antibodies., PMID:26407911

Clinical impact of preformed donor-specific denatured class I HLA antibodies after kidney transplantation., PMID:25683727

Complement component C3 activation: the leading cause of the prozone phenomenon affecting HLA antibody detection on single-antigen beads., PMID:24621535

The complement interference phenomenon as a cause for sharp fluctuations of serum anti-HLA antibody strength in kidney transplant patients., PMID:24056164

Frequent development of subclinical chronic antibody-mediated rejection within 1 year after renal transplantation with pre-transplant positive donor-specific antibodies and negative CDC crossmatches., PMID:23792054

Impact of alloantibody strength in crossmatch negative DSA positive kidney transplantation., PMID:23726814

Preformed complement-activating low-level donor-specific antibody predicts early antibody-mediated rejection in renal allografts., PMID:23197178

Significance of low-level DSA detected by solid-phase assay in association with acute and chronic antibody-mediated rejection., PMID:22764746

Mastering the risk of HLA antibodies in kidney transplantation: an algorithm based on pretransplant single-antigen flow bead techniques., PMID:21668626

Pretransplant IgG subclasses of donor-specific human leukocyte antigen antibodies and development of antibody-mediated rejection., PMID:21637140

Understanding the significance of low-level preformed donor-specific anti-HLA antibodies in renal transplant patients., PMID:22755432

C4d-fixing capability of low-level donor-specific HLA antibodies is not predictive for early antibody-mediated rejection., PMID:20395886

Utility of the virtual crossmatch in solid organ transplantation., PMID:19741534

Clinical relevance of pretransplant donor-specific HLA antibodies detected by single-antigen flow-beads., PMID:19502960

Low SAFB levels are associated with worse outcome in breast cancer patients., PMID:19137425

Alternative RNA splicing complexes containing the scaffold attachment factor SAFB2., PMID:17200140

Characterization of two Autographa californica nucleopolyhedrovirus proteins, Ac145 and Ac150, which affect oral infectivity in a host-dependent manner., PMID:15163737

Immunogenic properties of the Salmonella atypical fimbriae in BALB/c mice., PMID:15063568

Structure and dynamics of hnRNP-labelled nuclear bodies induced by stress treatments., PMID:11058091

A novel hnRNP protein (HAP/SAF-B) enters a subset of hnRNP complexes and relocates in nuclear granules in response to heat shock., PMID:10212141

Purification and molecular cloning of the scaffold attachment factor B (SAF-B), a novel human nuclear protein that specifically binds to S/MAR-DNA., PMID:8600450

Datasheet

Document Download

Anti-SAFB Antibody (R2G39).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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Anti-SAFB Antibody (R2G39) [RHH09601]
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