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Anti-PRKCE Antibody (R2C12)

Catalog #:   RHF92002 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: FCM, IF, IHC, WB
Accession: Q02156
Overview

Catalog No.

RHF92002

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000

Target

PRKCE, PKCE, nPKC-epsilon, Protein kinase C epsilon type

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q02156

Applications

FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R2C12

Data Image
  • Western blot
    Western blot analysis of PKC epsilon expression in Jurkat cell lysate.
  • Western blot
    All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
  • Western blot
    All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
References

Activated Protein Kinase C (PKC) Is Persistently Trafficked with Epidermal Growth Factor (EGF) Receptor., PMID:32906765

SHORT syndrome due to a novel de novo mutation in PRKCE (Protein Kinase Cɛ) impairing TORC2-dependent AKT activation., PMID:28934384

PKC-epsilon and TLR4 synergistically regulate resistin-mediated inflammation in human macrophages., PMID:28286252

PKCε-CREB-Nrf2 signalling induces HO-1 in the vascular endothelium and enhances resistance to inflammation and apoptosis., PMID:25883219

Antiphospholipid Antibodies Alter Cell-Death-Regulating Lipid Metabolites in First and Third Trimester Human Placentae., PMID:25856778

c-Abl modulates tumor cell sensitivity to antibody-dependent cellular cytotoxicity., PMID:25300860

T/B-cell interactions are more transient in response to weak stimuli in SLE-prone mice., PMID:25209945

Expression of Ser729 phosphorylated PKCepsilon in experimental crescentic glomerulonephritis: an immunohistochemical study., PMID:24998921

Rho kinase acts as a downstream molecule to participate in protein kinase Cε regulation of vascular reactivity after hemorrhagic shock in rats., PMID:24827390

Transcriptomic analysis of placenta affected by antiphospholipid antibodies: following the TRAIL of trophoblast death., PMID:22542907

Matrix metalloproteinases modulated by protein kinase Cε mediate resistin-induced migration of human coronary artery smooth muscle cells., PMID:21277149

The development of activating and inhibiting camelid VHH domains against human protein kinase C epsilon., PMID:21220006

Chronic stress sensitizes rats to pancreatitis induced by cerulein: role of TNF-α., PMID:21105189

Protease-activated receptor 2-mediated protection of myocardial ischemia-reperfusion injury: role of transient receptor potential vanilloid receptors., PMID:19812353

Diverse roles for protein kinase C delta and protein kinase C epsilon in the generation of high-fat-diet-induced glucose intolerance in mice: regulation of lipogenesis by protein kinase C delta., PMID:19809797

Sequential activation of protein kinase C isoforms by organic dust is mediated by tumor necrosis factor., PMID:19635931

Opposite effects of protein kinase C beta1 (PKCbeta1) and PKCepsilon in the metastatic potential of a breast cancer murine model., PMID:19132529

Increased sensitivity of desensitized TRPV1 by PMA occurs through PKCepsilon-mediated phosphorylation at S800., PMID:16564619

Nutrient regulation of PKCepsilon is mediated by leucine, not insulin, in skeletal muscle., PMID:15886222

Endothelin-1 regulates protein kinase C isoforms differently in smooth muscle cells and in cardiomyocytes., PMID:15838306

Protein kinase C epsilon regulates tumor necrosis factor-alpha-induced stannin gene expression., PMID:15798003

Protein kinase C isozymes in hypertension and hypertrophy: insight from SHHF rat hearts., PMID:15792354

The noble gas xenon induces pharmacological preconditioning in the rat heart in vivo via induction of PKC-epsilon and p38 MAPK., PMID:15644876

Protein kinase Cepsilon is dispensable for TCR/CD3-signaling., PMID:15589318

Identification of the linker histone H1 as a protein kinase Cepsilon-binding protein in vascular smooth muscle., PMID:15499382

Mechanism of oleic acid-induced gap junctional disassembly in rat cardiomyocytes., PMID:15350848

Protein kinase C-alpha-induced hypertrophy of neonatal rat ventricular myocytes., PMID:15271671

Phorbol ester-induced translocation of PKC epsilon to the nucleus in fibroblasts: identification of nuclear PKC epsilon-associating proteins., PMID:15251432

G protein-coupled receptor-mediated phosphorylation of the activation loop of protein kinase D: dependence on plasma membrane translocation and protein kinase Cepsilon., PMID:15190080

Protein kinase Cdelta as gonadotropin-releasing hormone target isoenzyme in the alphaT3-1 gonadotrope cell line., PMID:15153754

Protein phosphorylation of human brain glutamic acid decarboxylase (GAD)65 and GAD67 and its physiological implications., PMID:15147202

Integrin signaling in inflammatory and neuropathic pain in the rat., PMID:14984413

State-specific monoclonal antibodies identify an intermediate state in epsilon protein kinase C activation., PMID:14761958

Differential implication of protein kinase C isoforms in cytotoxic T lymphocyte degranulation and TCR-induced Fas ligand expression., PMID:14645153

Kinome analysis of host response to mycobacterial infection: a novel technique in proteomics., PMID:14500469

Differential and regulated binding of cAMP-dependent protein kinase and protein kinase C isoenzymes to gravin in human model neurons: Evidence that gravin provides a dynamic platform for the localization for kinases during neuronal development., PMID:12857743

Activation of focal adhesion kinase by protein kinase C epsilon in neonatal rat ventricular myocytes., PMID:12829427

Protein kinase C isoforms in the enteric nervous system., PMID:12811573

State-dependent phosphorylation of epsilon-isozyme of protein kinase C in adult rat dorsal root ganglia after inflammation and nerve injury., PMID:12694383

The second phase activation of protein kinase C delta at late G1 is required for DNA synthesis in serum-induced cell cycle progression., PMID:12653960

Alterations in protein kinase C isoenzyme expression and autophosphorylation during the progression of pressure overload-induced left ventricular hypertrophy., PMID:12619877

A role for PKC-epsilon in Fc gammaR-mediated phagocytosis by RAW 264.7 cells., PMID:12499353

Association of protein kinase C with GABA(A) receptors containing alpha1 and alpha4 subunits in the cerebral cortex: selective effects of chronic ethanol consumption., PMID:12091471

Activation and phosphatidylinositol 3-kinase-dependent phosphorylation of protein kinase C-epsilon by the B cell antigen receptor., PMID:12036603

PKC epsilon is associated with myosin IIA and actin in fibroblasts., PMID:11897493

NK-active cytokines IL-2, IL-12, and IL-15 selectively modulate specific protein kinase C (PKC) isoforms in primary human NK cells., PMID:11788941

Isoform-specific membrane translocation of protein kinase C after ischemic preconditioning., PMID:11700956

B-cell receptor- and phorbol ester-induced NF-kappaB and c-Jun N-terminal kinase activation in B cells requires novel protein kinase C's., PMID:11533251

Regulation of ATP-sensitive K(+) channels by protein kinase C in murine colonic myocytes., PMID:11502563

Cis-polyunsaturated fatty acids stimulate beta1 integrin-mediated adhesion of human breast carcinoma cells to type IV collagen by activating protein kinases C-epsilon and -mu., PMID:11289113

Datasheet

Document Download

Anti-PRKCE Antibody (R2C12).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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Anti-PRKCE Antibody (R2C12) [RHF92002]
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