Catalog No.
RHF85801
Species reactivity
Human, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000
Target
Splicing factor SC35, Splicing factor, arginine/serine-rich 2, SC-35, Serine/arginine-rich splicing factor 2, SFRS2, SRSF2, Splicing component, 35 kDa, Protein PR264
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q01130
Applications
IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2B89
Long-term longitudinal analysis of 4,187 participants reveals insights into determinants of clonal hematopoiesis., PMID:39251642
Predicting recurrent glioblastoma clinical outcome to immune checkpoint inhibition and low-dose bevacizumab with tumor in situ fluid circulating tumor DNA analysis., PMID:39105794
Antibody-Based and Cell Therapies for Advanced Mastocytosis: Established and Novel Concepts., PMID:37894806
Using Proteome Microarray and Gene Expression Omnibus Database to Screen Tumour-Associated Antigens to Construct the Optimal Diagnostic Model of Oesophageal Squamous Cell Carcinoma., PMID:37433700
Low-Risk Myelodysplastic Syndrome Revisited: Morphological, Autoimmune, and Molecular Features as Predictors of Outcome in a Single Center Experience., PMID:35392224
Classification and Personalized Prognostic Assessment on the Basis of Clinical and Genomic Features in Myelodysplastic Syndromes., PMID:33539200
Identification of novel autoantibodies based on the protein chip encoded by cancer-driving genes in detection of esophageal squamous cell carcinoma., PMID:33457096
SON and SRRM2 are essential for nuclear speckle formation., PMID:33095160
Selection of Vaccinia Virus-Neutralizing Antibody from a Phage-Display Human-Antibody Library., PMID:30856707
RUNX1 Mutations Can Lead to Aberrant Expression of CD79a and PAX5 in Acute Myelogenous Leukemias: A Potential Diagnostic Pitfall., PMID:30396184
Transcriptome Analysis of Monozygotic Twin Brothers with Childhood Primary Myelofibrosis., PMID:28185911
Exercise capacity in chronic heart failure patients is related to active gene transcription in skeletal muscle and not apoptosis., PMID:19318954
Cajal bodies and interchromatin granule clusters in cricket oocytes: composition, dynamics and interactions., PMID:17123844
Basonuclins 1 and 2, whose genes share a common origin, are proteins with widely different properties and functions., PMID:16891417
Mimicking phosphorylation of the small heat-shock protein alphaB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles., PMID:15511225
Clustering of multiple specific genes and gene-rich R-bands around SC-35 domains: evidence for local euchromatic neighborhoods., PMID:12975345
Nuclear staining for the small heat shock protein alphaB-crystallin colocalizes with splicing factor SC35., PMID:12924631
Nuclear distribution of Oct-4 transcription factor in transcriptionally active and inactive mouse oocytes and its relation to RNA polymerase II and splicing factors., PMID:12858338
Nucleocytoplasmic transport of proteins and poly(A)+ RNA in reconstituted Tpr-less nuclei in living mammalian cells., PMID:11952838
Further considerations on the intranuclear distribution of HMGI/Y proteins., PMID:11767201
Distinct changes in intranuclear lamin A/C organization during myoblast differentiation., PMID:11739632
Novel N-terminal variant of human VDR., PMID:11518809
PRCC, the commonest TFE3 fusion partner in papillary renal carcinoma is associated with pre-mRNA splicing factors., PMID:11313942
Nuclear distribution of RNA polymerase II in human oocytes from antral follicles: dynamics relative to the transcriptional state and association with splicing factors., PMID:10771521
A human importin-beta family protein, transportin-SR2, interacts with the phosphorylated RS domain of SR proteins., PMID:10713112
Intron-independent association of splicing factors with active genes., PMID:10366587
Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements., PMID:10022872
Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy., PMID:9712158
A comparative nuclear localization study of galectin-1 with other splicing components., PMID:9683529
The expression of the essential nuclear splicing factor SC35 is altered by human immunodeficiency virus infection., PMID:9617768
Subcellular localization of human immunodeficiency virus type 1 RNAs, Rev, and the splicing factor SC-35., PMID:9601515
Association of phosphorylated serine/arginine (SR) splicing factors with the U1-small ribonucleoprotein (snRNP) autoantigen complex accompanies apoptotic cell death., PMID:9463405
Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing., PMID:9447963
Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles., PMID:9446799
A covalent fluorescent-gold immunoprobe: simultaneous detection of a pre-mRNA splicing factor by light and electron microscopy., PMID:9212820
Structural protein 4.1 in the nucleus of human cells: dynamic rearrangements during cell division., PMID:9128242
Quantitative digital analysis of diffuse and concentrated nuclear distributions of nascent transcripts, SC35 and poly(A)., PMID:9056409
Transcription-dependent redistribution of nuclear protein 4.1 to SC35-enriched nuclear domains., PMID:9044054
Factors involved in the activation of pre-mRNA splicing from downstream splicing enhancers., PMID:8864844
Fine structural cytochemical and immunocytochemical analysis of nucleic acids and ribonucleoprotein distribution in nuclei of pig oocytes and early preimplantation embryos., PMID:8662249
An amino acid sequence motif sufficient for subnuclear localization of an arginine/serine-rich splicing factor., PMID:8524796
Localization of the glucocorticoid receptor in discrete clusters in the cell nucleus., PMID:8537440
Colocalization of a high molecular mass phosphoprotein of the nuclear matrix (p255) with spliceosomes., PMID:7657711
Influenza virus NS1 protein alters the subnuclear localization of cellular splicing components., PMID:9049349
Splicing components are excluded from the transcriptionally inactive XY body in male meiotic nuclei., PMID:7696714
Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors., PMID:8294510
Nuclear organization of splicing snRNPs during differentiation of murine erythroleukemia cells in vitro., PMID:8245117
Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus., PMID:8320255
For research use only. Not for human or drug use.
