Catalog No.
RHE90202
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
WB: 1:1000-1:2000
Target
NLK1, NEK2, NimA-like protein kinase 1, HSPK 21, NEK2A, Serine/threonine-protein kinase Nek2, NimA-related protein kinase 2, Never in mitosis A-related kinase 2
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P51955
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1Y49
NEK2 regulates B cell function and the severity of experimental autoimmune encephalomyelitis., PMID:40481479
Phosphorylation of the prolyl isomerase Cyclophilin A regulates its localisation and release from the centrosome during mitosis., PMID:36691345
Centromere protein F promotes progression of hepatocellular carcinoma through ERK and cell cycle-associated pathways., PMID:34857915
Preparation of an anti-NEK2 monoclonal antibody and its application in liver cancer., PMID:34706700
COL1A1: A potential therapeutic target for colorectal cancer expressing wild-type or mutant KRAS., PMID:30132520
Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays., PMID:29468550
CDC25B is involved in the centrosomal microtubule nucleation in two-cell stage mouse embryos., PMID:27885657
Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins., PMID:27486244
Nek2 phosphorylates and stabilizes β-catenin at mitotic centrosomes downstream of Plk1., PMID:24501426
Fanconi anemia complementation group A (FANCA) localizes to centrosomes and functions in the maintenance of centrosome integrity., PMID:23806870
Cdk4 and nek2 signal binucleation and centrosome amplification in a her2+ breast cancer model., PMID:23776583
Tumor antigen analysis in neuroblastoma by serological interrogation of bioinformatic data., PMID:20718755
Identification by high-throughput screening of viridin analogs as biochemical and cell-based inhibitors of the cell cycle-regulated nek2 kinase., PMID:20664067
Identification of molecular targets associated with transformed diffuse large B cell lymphoma using highly purified tumor cells., PMID:19844990
The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability., PMID:18297113
[Interaction between a novel centrosomal protein TACP1 and mitotic kinase Nek2A]., PMID:17717823
Development and comparison of nonradioactive in vitro kinase assays for NIMA-related kinase 2., PMID:16962550
Tumour-associated Proteins in Oral Squamous Cell Carcinomas by Proteomics., PMID:31394652
Identification, cloning and characterization of a plasma membrane zinc efflux transporter, TrZnT-1, from fugu pufferfish (Takifugu rubripes)., PMID:16212555
Nek2A kinase regulates the localization of numatrin to centrosome in mitosis., PMID:15388344
Inhibition of centrosome separation after DNA damage: a role for Nek2., PMID:15387139
Xenopus cold-inducible RNA-binding protein 2 interacts with ElrA, the Xenopus homolog of HuR, and inhibits deadenylation of specific mRNAs., PMID:13679363
Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles., PMID:12857871
Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation., PMID:12386167
DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubule-organizing centers., PMID:11956323
Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators., PMID:10224116
C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2., PMID:9647649
The in vivo expression pattern of mouse Nek2, a NIMA-related kinase, indicates a role in both mitosis and meiosis., PMID:9434622
For research use only. Not for human or drug use.
