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Anti-RANGAP1 Antibody (R1W73)

Catalog #:   RHE46501 Specific References (21) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, IHC, IP, WB
Accession: P46060
Overview

Catalog No.

RHE46501

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000

Target

SD, RANGAP1, Ran GTPase-activating protein 1, RanGAP1, KIAA1835

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P46060

Applications

IF, IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1W73

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of RanGAP1 (green) in hela using RanGAP1 antibody,and DAPI(blue)
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human tonsil using RanGAP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot
    Western blot analysis of RanGAP1 in 3T3 lysates using RanGAP1 antibody.
  • Western blot
    Western blot analysis of RanGAP1 in Hela, A549, HL-60, U2OS, C6 lysates using RanGAP1 antibody.
References

SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate., PMID:36494414

Recruitment of Host Nuclear Pore Components to the Vicinity of Theileria Schizonts., PMID:32024710

SGK1 affects RAN/RANBP1/RANGAP1 via SP1 to play a critical role in pre-miRNA nuclear export: a new route of epigenomic regulation., PMID:28358001

ON 01910.Na inhibits growth of diffuse large B-cell lymphoma by cytoplasmic sequestration of sumoylated C-MYB/TRAF6 complex., PMID:27150054

PCGF2 negatively regulates arsenic trioxide-induced PML-RARA protein degradation via UBE2I inhibition in NB4 cells., PMID:27030546

Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores., PMID:22331847

Tollip is a mediator of protein sumoylation., PMID:19198660

Regulation of Wnt signaling by the nuclear pore complex., PMID:18439914

The Nup358-RanGAP complex is required for efficient importin alpha/beta-dependent nuclear import., PMID:18305100

Chip-based analysis of SUMO (small ubiquitin-like modifier) conjugation to a target protein., PMID:16820290

The SUMO pathway is essential for nuclear integrity and chromosome segregation in mice., PMID:16326389

Roles of Ran-GTP and Ran-GDP in precursor vesicle recruitment and fusion during nuclear envelope assembly in a human cell-free system., PMID:11231159

A novel mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localized in the nucleolus at interphase., PMID:11029585

Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3., PMID:10692421

SUMO-1 modification of the acute promyelocytic leukaemia protein PML: implications for nuclear localisation., PMID:9885291

A plant homolog of the neutrophil NADPH oxidase gp91phox subunit gene encodes a plasma membrane protein with Ca2+ binding motifs., PMID:9490748

Evidence for covalent modification of the nuclear dot-associated proteins PML and Sp100 by PIC1/SUMO-1., PMID:9412458

RanBP2 associates with Ubc9p and a modified form of RanGAP1., PMID:9108047

A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2., PMID:9019411

A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex., PMID:8978815

Direct and indirect association of the small GTPase ran with nuclear pore proteins and soluble transport factors: studies in Xenopus laevis egg extracts., PMID:8885229

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

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Anti-RANGAP1 Antibody (R1W73) [RHE46501]
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