Catalog No.
RHE29801
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000
Target
Aquaporin-2, AQP-CD, AQP2, Water channel protein for renal collecting duct, AQP-2, WCH-CD, Collecting duct water channel protein, Aquaporin-CD, ADH water channel
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P41181
Applications
IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1V85
Focal Adhesion Kinase (FAK) inhibition induces membrane accumulation of aquaporin2 (AQP2) through endocytosis inhibition and actin depolymerization in renal epithelial cells., PMID:39416213
Optimizing renal transporter immunodetection: consequences of freeze-thaw during sample preparation., PMID:39205660
Cannabinoid receptor type 1 activation causes a water diuresis by inducing an acute central diabetes insipidus in mice., PMID:38634131
Collecting duct water permeability inhibition by EGF is associated with decreased cAMP, PKA activity, and AQP2 phosphorylation at Ser269., PMID:38205543
Structural analysis of the water channel AQP2 by single-particle cryo-EM., PMID:37315821
Novel concept of Wilms' tumor development: involvement of pluripotential cells of ureteric bud., PMID:37209922
Identification of protein kinase A signalling molecules in renal collecting ducts., PMID:37013848
Intracellular sites of AQP2 S256 phosphorylation identified using inhibitors of the AQP2 recycling itinerary., PMID:36454701
Urinary reabsorption in the rat kidney by anticholinergics., PMID:33911165
Phosphorylation profile of human AQP2 in urinary exosomes by LC-MS/MS phosphoproteomic analysis., PMID:32529500
Uterine and placental specific localization of AQP2 and AQP8 is related with changes of serum progesterone levels in pregnant queens., PMID:31593882
Site-1 protease-derived soluble (pro)renin receptor targets vasopressin receptor 2 to enhance urine concentrating capability., PMID:30944256
Genome-Wide Mapping of DNA Accessibility and Binding Sites for CREB and C/EBPβ in Vasopressin-Sensitive Collecting Duct Cells., PMID:29572403
AQP2 in human urine is predominantly localized to exosomes with preserved water channel activities., PMID:29396622
Ezrin directly interacts with AQP2 and promotes its endocytosis., PMID:28754689
Effects of gonadectomy and testosterone treatment on aquaporin expression in the kidney of normotensive and hypertensive rats., PMID:28399644
Targeting gene expression to specific cells of kidney tubules in vivo, using adenoviral promoter fragments., PMID:28253301
Hypercalcemia induces targeted autophagic degradation of aquaporin-2 at the onset of nephrogenic diabetes insipidus., PMID:28139295
Disruption of Membranes of Extracellular Vesicles Is Necessary for ELISA Determination of Urine AQP2: Proof of Disruption and Epitopes of AQP2 Antibodies., PMID:27681727
β3 adrenergic receptor in the kidney may be a new player in sympathetic regulation of renal function., PMID:27206969
An immunoassay for urinary extracellular vesicles., PMID:26823283
Dot1l deficiency leads to increased intercalated cells and upregulation of V-ATPase B1 in mice., PMID:26404731
Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture., PMID:26147297
Role of angiotensin II and oxidative stress on renal aquaporins expression in hypernatremic rats., PMID:24590923
Elevated cAMP increases aquaporin-3 plasma membrane diffusion., PMID:24452376
Vasopressin induces apical expression of caveolin in rat kidney collecting duct principal cells., PMID:24133120
Quantification of human urinary exosomes by nanoparticle tracking analysis., PMID:24060994
Immunolocalization of aquaporin water channels in the domestic cat male genital tract., PMID:23826797
Application of phage display for ligand peptidomics to identify peptide ligands binding to AQP2-expressing membrane fractions., PMID:23765627
Long-term vasopressin-V2-receptor stimulation induces regulation of aquaporin 4 protein in renal inner medulla and cortex of Brattleboro rats., PMID:23615760
Proposed mechanism in the change of cellular composition in the outer medullary collecting duct during potassium homeostasis., PMID:23059887
Decreased expression of aquaporin 2 in the collecting duct of mice lacking the vasopressin V1a receptor., PMID:22968856
Vasopressin increases S261 phosphorylation in AQP2-P262L, a mutant in recessive nephrogenic diabetes insipidus., PMID:22778181
Immunofluorescent localization of the Rab-GAP protein TBC1D4 (AS160) in mouse kidney., PMID:22466139
Effect of Poria cocos on hypertonic stress-induced water channel expression and apoptosis in renal collecting duct cells., PMID:22414475
Characterization of Aquaporin 4 Protein Expression and Localization in Tissues of the Dogfish (Squalus acanthias)., PMID:22363294
Architecture of kangaroo rat inner medulla: segmentation of descending thin limb of Henle's loop., PMID:22237592
Daily variance of urinary excretion of AQP2 determined by sandwich ELISA method., PMID:22160633
Immunohistochemical identification of kidney nephron segments in the dog, rat, mouse, and cynomolgus monkey., PMID:22006284
Identification of new signaling components in the sensory epithelium of human saccule., PMID:21886636
Slc26a11, a chloride transporter, localizes with the vacuolar H(+)-ATPase of A-intercalated cells of the kidney., PMID:21716257
Functional characterization of three aquaglyceroporins from Trypanosoma brucei in osmoregulation and glycerol transport., PMID:21471730
Molecular mechanisms of angiotensin II stimulation on aquaporin-2 expression and trafficking., PMID:21325494
Routes of epithelial water flow: aquaporins versus cotransporters., PMID:21112289
Have we finally identified an autoimmune demyelinating disease?, PMID:20437595
Quantitative analysis of aquaporin-2 phosphorylation., PMID:20089674
Amiloride blocks lithium entry through the sodium channel thereby attenuating the resultant nephrogenic diabetes insipidus., PMID:19367330
Potential role of purinergic signaling in lithium-induced nephrogenic diabetes insipidus., PMID:19244398
Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry., PMID:19209902
Effects of receptor-mediated endocytosis and tubular protein composition on volume retention in experimental glomerulonephritis., PMID:19193726
For research use only. Not for human or drug use.
