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Anti-GJB1 Antibody (R1L32)

Catalog #:   RHC29601 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: FCM, IHC, WB
Accession: P08034
Overview

Catalog No.

RHC29601

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

FCM: 1:20-1:100, IHC: 1:100-1:200, WB: 1:1000-1:2000

Target

GAP junction 28 kDa liver protein, Cx32, Connexin-32, Gap junction beta-1 protein, CX32, GJB1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P08034

Applications

FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1L32

Data Image
  • Western blot
    Western blot analysis of GJB1 expression in MCF-7 cell lysate.
References

A dose escalation and safety study of AAVrh10-mediated Schwann cell-targeted gene therapy for CMT1X., PMID:40055046

A fully human IgG1 antibody targeting connexin 32 extracellular domain blocks CMTX1 hemichannel dysfunction in an in vitro model., PMID:39639332

Generation of Connexin-Expressing Stable Cell Pools., PMID:38578420

Investigating oligodendrocyte connexins: Heteromeric interactions between Cx32 and mutant or wild-type forms of Cx47 do not contribute to or modulate gap junction function., PMID:33835612

Genetic biomarkers for intravenous immunoglobulin response in chronic inflammatory demyelinating polyradiculoneuropathy., PMID:33460483

Antibodies against nodo-paranodal proteins are not present in genetic neuropathies., PMID:32102977

Localization of Retinal Ca2+/Calmodulin-Dependent Kinase II-β (CaMKII-β) at Bipolar Cell Gap Junctions and Cross-Reactivity of a Monoclonal Anti-CaMKII-β Antibody With Connexin36., PMID:31555090

Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis., PMID:26655499

Endothelial connexin 32 regulates tissue factor expression induced by inflammatory stimulation and direct cell-cell interaction with activated cells., PMID:25171777

Synchronized necrotic death of attached hepatocytes mediated via gap junctions., PMID:24893927

Endothelial connexin32 enhances angiogenesis by positively regulating tube formation and cell migration., PMID:24333598

Connexin47 protein phosphorylation and stability in oligodendrocytes depend on expression of Connexin43 protein in astrocytes., PMID:23637189

A family with IVIg-responsive Charcot-Marie-Tooth disease., PMID:23232577

Connexin 36 is expressed in beta and connexins 26 and 32 in acinar cells at the end of the secondary transition of mouse pancreatic development and increase during fetal and perinatal life., PMID:22505190

Inflammatory demyelinating CNS disorder in a case of X-linked Charcot-Marie-Tooth disease: positive response to natalizumab., PMID:22411047

Ablation of connexin30 in transgenic mice alters expression patterns of connexin26 and connexin32 in glial cells and leptomeninges., PMID:22098503

Semi-automatic counting of connexin 32s immunolocalized in cultured fetal rat hepatocytes using image processing., PMID:21764105

In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors., PMID:21536677

Connexin implication in the control of the murine beta-cell mass., PMID:21527868

Connexin32 protects against vascular inflammation by modulating inflammatory cytokine expression by endothelial cells., PMID:21036166

A novel GJB1 frameshift mutation produces a transient CNS symptom of X-linked Charcot-Marie-Tooth disease., PMID:20857133

Cytoplasmic accumulation of connexin32 expands cancer stem cell population in human HuH7 hepatoma cells by enhancing its self-renewal., PMID:20209499

Tissue-specific cross-reactivity of connexin32 antibodies: problems and solutions unique to the central nervous system., PMID:19845480

Connexin32 is expressed in vascular endothelial cells and participates in gap-junction intercellular communication., PMID:19265674

Extracellular-loop peptide antibodies reveal a predominant hemichannel organization of connexins in polarized intestinal cells., PMID:18267319

Gap junction protein expression and cellularity: comparison of immature and adult equine digital tendons., PMID:17848160

Study of antibodies to PMP22, IL-6 and TNF-alpha concentrations in serum in a CMTX1 family., PMID:17714866

Protective role of connexin 32 in steady-state hematopoiesis, regeneration state, and leukemogenesis., PMID:17463168

The gap junction protein connexin32 interacts with the Src homology 3/hook domain of discs large homolog 1., PMID:17284442

Expression of protein 4.1G in Schwann cells of the peripheral nervous system., PMID:16752423

X-linked Charcot-Marie-Tooth disease and progressive-relapsing central demyelinating disease., PMID:16301507

Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning., PMID:16203097

Expression of connexins 26, 32 and 43 in the human colon--an immunohistochemical study., PMID:15704645

Tight junction protein MAGI-1 is up-regulated by transfection with connexin 32 in an immortalized mouse hepatic cell line: cDNA microarray analysis., PMID:15558322

Developmental expression patterns and regulation of connexins in the mouse mammary gland: expression of connexin30 in lactogenesis., PMID:15517403

Unique distributions of the gap junction proteins connexin29, connexin32, and connexin47 in oligodendrocytes., PMID:15293232

Gap junctions are required for trophoblast proliferation in early human placental development., PMID:15193866

Coexistent hereditary and inflammatory neuropathy., PMID:14607795

Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice., PMID:14603462

Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system., PMID:12900929

ECM-induced gap junctional communication enhances mammary epithelial cell differentiation., PMID:12893812

Effect of growth hormone (GH) on in vitro nuclear and cytoplasmic oocyte maturation, cumulus expansion, hyaluronan synthases, and connexins 32 and 43 expression, and GH receptor messenger RNA expression in equine and porcine species., PMID:12773434

Gap junctions do not underlie changes in whole-cell conductance in anoxic turtle brain., PMID:12507621

Connexin29 expression, immunocytochemistry and freeze-fracture replica immunogold labelling (FRIL) in sciatic nerve., PMID:12372015

Connexin29 is uniquely distributed within myelinating glial cells of the central and peripheral nervous systems., PMID:12151525

Connexin 43 enhances the adhesivity and mediates the invasion of malignant glioma cells., PMID:12040035

Characteristic distribution of gap junctions in rat lacrimal gland in vivo and reconstruction of a gap junction in an in vitro model., PMID:12003240

Dilated bile canaliculi and attenuated decrease of nerve-dependent bile secretion in connexin32-deficient mouse liver., PMID:11680630

Reduction of malignant phenotype of HEPG2 cell is associated with the expression of connexin 26 but not connexin 32., PMID:11576997

A spontaneously arising mutation in connexin32 with repeated passage of FRTL-5 cells coincides with increased growth rate and reduced thyroxine release., PMID:11564600

Datasheet

Document Download

Anti-GJB1 Antibody (R1L32).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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Anti-GJB1 Antibody (R1L32) [RHC29601]
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