Catalog No.
RHB86911
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IHC: 1:50-1:100, WB: 1:500-1:1000
Target
HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P00533
Applications
IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1H30
CD148 agonistic antibody alleviates renal injury induced by chronic angiotensin II infusion in mice., PMID:40165104
Epidermal growth factor potentiates EGFR(Y992/1173)-mediated therapeutic response of triple negative breast cancer cells to cold atmospheric plasma-activated medium., PMID:38052106
In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-detection of Phosphorylated EGFR in A431 Cultured Cells., PMID:36513933
The role of anlotinib-mediated EGFR blockade in a positive feedback loop of CXCL11-EGF-EGFR signalling in anaplastic thyroid cancer angiogenesis., PMID:34088989
Inhibition of p70 isoforms of S6K1 induces anoikis to prevent transformed human hepatocyte growth., PMID:33189822
Autophagy processes are dependent on EGF receptor signaling., PMID:30100990
3D Cell-Based High-Content Screening (HCS) Using a Micropillar and Microwell Chip Platform., PMID:29889500
Dual "mAb" HER family blockade in head and neck cancer human cell lines combined with photon therapy., PMID:28939847
ΔNp63 activates EGFR signaling to induce loss of adhesion in triple-negative basal-like breast cancer cells., PMID:28349272
Cetuximab treatment alters the content of extracellular vesicles released from tumor cells., PMID:27021928
A Chimeric Cetuximab-Functionalized Corona as a Potent Delivery System for Microtubule-Destabilizing Nanocomplexes to Hepatocellular Carcinoma Cells: A Focus on EGFR and Tubulin Intracellular Dynamics., PMID:26426829
Xenograft assessment of predictive biomarkers for standard head and neck cancer therapies., PMID:25619980
High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system., PMID:24577528
Fhit regulates EMT targets through an EGFR/Src/ERK/Slug signaling axis in human bronchial cells., PMID:24464917
Sym004, a novel EGFR antibody mixture, can overcome acquired resistance to cetuximab., PMID:24204198
Increased expression of heat shock protein 70 in chronic obstructive pulmonary disease., PMID:24095952
Protein phosphorylation profiling using an in situ proximity ligation assay: phosphorylation of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 in lung cancer cells., PMID:23520446
Functional cooperativity by direct interaction between PAK4 and MMP-2 in the regulation of anoikis resistance, migration and invasion in glioma., PMID:23254288
Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells., PMID:23251234
Monitoring of circulating tumor cells and their expression of EGFR/phospho-EGFR during combined radiotherapy regimens in locally advanced squamous cell carcinoma of the head and neck., PMID:22583603
A simple multicolor flow cytometry protocol for detection and molecular characterization of circulating tumor cells in epithelial cancers., PMID:22438318
Phase II trial of cetuximab in patients with metastatic or locally advanced soft tissue or bone sarcoma., PMID:22237145
In situ analysis of mutant EGFRs prevalent in glioblastoma multiforme reveals aberrant dimerization, activation, and differential response to anti-EGFR targeted therapy., PMID:22232519
EGFR- and VEGF(R)-targeted small molecules show synergistic activity in colorectal cancer models refractory to combinations of monoclonal antibodies., PMID:21880790
Coactivation of receptor tyrosine kinases in malignant mesothelioma as a rationale for combination targeted therapy., PMID:21774103
Radiosynthesis and initial in vitro evaluation of [18F]F-PEG6-IPQA--a novel PET radiotracer for imaging EGFR expression-activity in lung carcinomas., PMID:20859697
Novel monoclonal antibodies recognizing the active conformation of epidermal growth factor receptor., PMID:20226763
Enhanced phosphorylation of the epidermal growth factor receptor at the site of tyrosine 992 in esophageal carcinomas., PMID:19133013
Phospho-Akt pathway activation and inhibition depends on N-cadherin or phospho-EGFR expression in invasive human bladder cancer cell lines., PMID:19070520
Phosphorylated EGFR and PI3K/Akt signaling kinases are expressed in circulating tumor cells of breast cancer patients., PMID:18822183
Activation of platelet-activating factor receptor and pleiotropic effects on tyrosine phospho-EGFR/Src/FAK/paxillin in ovarian cancer., PMID:18632638
Immunohistochemical analysis of phosphorylated epidermal growth factor receptor might provide a surrogate marker of EGFR mutation., PMID:18585821
Individualized Survival and Treatment Response Predictions in Breast Cancer Patients: Involvements of Phospho-EGFR and Phospho-Her2/neu Proteins., PMID:25558292
Immune activation by epidermal growth factor receptor specific monoclonal antibody therapy for head and neck cancer., PMID:18086972
c-Met activation in lung adenocarcinoma tissues: an immunohistochemical analysis., PMID:17459054
Individualized survival and treatment response predictions for breast cancers using phospho-EGFR, phospho-ER, phospho-HER2/neu, phospho-IGF-IR/In, phospho-MAPK, and phospho-p70S6K proteins., PMID:17393355
Inhibition of the epidermal growth factor receptor enhances castration-induced prostate involution and reduces testosterone-stimulated prostate growth in adult rats., PMID:17252557
In vivo and in vitro antitumor activity of oxaliplatin in combination with cetuximab in human colorectal tumor cell lines expressing different level of EGFR., PMID:16320055
Assessment of Epidermal Growth Factor Receptor (EGFR) signaling in paired colorectal cancer and normal colon tissue samples using computer-aided immunohistochemical analysis., PMID:16319527
Helicobacter pylori-stimulated interleukin-8 (IL-8) promotes cell proliferation through transactivation of epidermal growth factor receptor (EGFR) by disintegrin and metalloproteinase (ADAM) activation., PMID:16240219
Prediction of active drug plasma concentrations achieved in cancer patients by pharmacodynamic biomarkers identified from the geo human colon carcinoma xenograft model., PMID:16061873
Immunohistochemically determined total epidermal growth factor receptor levels not of prognostic value in newly diagnosed glioblastoma multiforme: report from the Radiation Therapy Oncology Group., PMID:15890570
Peroxynitrite targets the epidermal growth factor receptor, Raf-1, and MEK independently to activate MAPK., PMID:10801894
For research use only. Not for human or drug use.
