Catalog No.
KVV99903
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. SARS-CoV-2 Spike has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Spike Mouse IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Goat Anti-Mouse IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 Spike Mouse IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-SARS-CoV-2 Spike Mouse IgG concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative (Competitive)
Range
50 - 3,200 pg/mL
Sensitivity
25.23 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (pg/mL) |
1463.2 |
410.2 |
186.0 |
1410.7 |
372.1 |
110.1 |
|
Standard deviation |
84.9 |
17.6 |
6.3 |
68.4 |
16.9 |
11.3 |
|
CV (%) |
5.8 |
4.3 |
3.4 |
4.9 |
4.6 |
10.3 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
Spike glycoprotein, S glycoprotein
For research use only. Not for human or drug use.
