Catalog No.
AHK16101
Biological activity
Measured by its binding ability in a functional ELISA. When biotinylated Sialyl Lewis X was immobilized at 0.05 μg/mL (100 μL/well), it binds recombinant human SIGLEC8/SAF-2 (Cat #: AHK16101). The concentration of human SIGLEC8/SAF-2 that produces 50% of the optimal binding response is approximately 0.2-1.2 μg/mL.
Expression system
Mammalian Cells
Species
Homo sapiens (Human)
Protein length
Met17-Ala363
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9NYZ4
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
SAF2, Sialoadhesin family member 2, SAF-2, Siglec-8, SIGLEC8, Sialic acid-binding Ig-like lectin 8
Enhanced Siglec-8 and HLA-DR and reduced CRTH2 surface expression highlight a distinct phenotypic signature of circulating eosinophils in atopic dermatitis., PMID:39998842
Understanding the Glycosylation Pathways Involved in the Biosynthesis of the Sulfated Glycan Ligands for Siglecs., PMID:39836965
Vectorized Human Antibody-Mediated Anti-Eosinophil Gene Therapy., PMID:39725494
Increased autoreactivity and maturity of EBI2+ antibody-secreting cells from nasal polyps., PMID:39253973
Sialylated keratan sulfates on MUC5B are Siglec-8 ligands in the human esophagus., PMID:39173029
Regulation of mast cells by overlapping but distinct protein interactions of Siglec-6 and Siglec-8., PMID:38186079
IL-13 and IL-13-induced periostin levels are specifically decreased in patients following endoscopic sinus surgery for chronic rhinosinusitis., PMID:38157944
Human CD79b+ neutrophils in the blood are associated with early-stage melanoma., PMID:38022639
Tetra- and Hexavalent Siglec-8 Ligands Modulate Immune Cell Activation., PMID:37947772
Interactions between Siglec-8 and endogenous sialylated cis ligands restrain cell death induction in human eosinophils and mast cells., PMID:37928558
Mast cell silencing: A novel therapeutic approach for urticaria and other mast cell-mediated diseases., PMID:37605867
Siglecs as potential targets of therapy in human mast cell- and/or eosinophil-associated diseases., PMID:37413923
Safety and efficacy of lirentelimab in patients with refractory indolent systemic mastocytosis: a first-in-human clinical trial., PMID:37290787
Integrated Bioinformatics Analysis for the Identification of Key lncRNAs, mRNAs, and Potential Drugs in Clear Cell Renal Cell Carcinomas., PMID:37275334
Biomarkers for predicting type 2-high and uncontrolled asthma in real-world practice., PMID:37209833
Developments and perspectives in allergology., PMID:37070510
Eosinophil Subtypes in Adults with Asthma and Adults with Chronic Obstructive Pulmonary Disease., PMID:37071848
Jak out of the box: Targeting Bruton's tyrosine kinase, sialic acid-binding immunoglobulin-like lectin-8, and Janus kinase 1 in food allergy., PMID:36738782
Human Lung Mast Cells: Therapeutic Implications in Asthma., PMID:36430941
Novel peanut-specific human IgE monoclonal antibodies enable screens for inhibitors of the effector phase in food allergy., PMID:36248809
Computationally guided conversion of the specificity of E-selectin to mimic that of Siglec-8., PMID:36191232
A new approach for extracting information from protein dynamics., PMID:36094321
Pharmacological Induction of Granulocyte Cell Death as Therapeutic Strategy., PMID:36028226
Uncovering Signals of Positive Selection in Peruvian Populations from Three Ecological Regions., PMID:35860855
Treatment Trends for Eosinophilic Esophagitis and the Other Eosinophilic Gastrointestinal Diseases: Systematic Review of Clinical Trials., PMID:35654734
Human brain sialoglycan ligand for CD33, a microglial inhibitory Siglec implicated in Alzheimer's disease., PMID:35452678
Functional and Phenotypic Characterization of Siglec-6 on Human Mast Cells., PMID:35406705
Use of intraoperative frontal sinus mometasone-eluting stents decreased interleukin 5 and interleukin 13 in patients with chronic rhinosinusitis with nasal polyps., PMID:35362251
A new approach for extracting information from protein dynamics., PMID:35313540
Studies on activation and regulation of the coagulation cascade in chronic rhinosinusitis with nasal polyps., PMID:35271862
Anti-phospholipid antibodies are elevated and functionally active in chronic rhinosinusitis with nasal polyps., PMID:35253284
The Inhibitory Receptor Siglec-8 Interacts With FcεRI and Globally Inhibits Intracellular Signaling in Primary Mast Cells Upon Activation., PMID:35154156
NLRC4 Inflammasome-Mediated Regulation of Eosinophilic Functions., PMID:35036029
Chemoenzymatic Synthesis of Sialyl Sulfo-Oligosaccharides as Potent Siglec-8 Ligands via Transglycosylation Catalyzed by Keratanase II., PMID:34914356
Siglec-8 Signals Through a Non-Canonical Pathway to Cause Human Eosinophil Death In Vitro., PMID:34721399
Understanding human mast cells: lesson from therapies for allergic and non-allergic diseases., PMID:34611316
Nanoparticles Displaying Allergen and Siglec-8 Ligands Suppress IgE-FcεRI-Mediated Anaphylaxis and Desensitize Mast Cells to Subsequent Antigen Challenge., PMID:33911007
Integrative Analysis of the Expression of SIGLEC Family Members in Lung Adenocarcinoma via Data Mining., PMID:33796453
Mast Cell and Eosinophil Activation Are Associated With COVID-19 and TLR-Mediated Viral Inflammation: Implications for an Anti-Siglec-8 Antibody., PMID:33777047
Induction of the endogenous sialoglycan ligand for eosinophil death receptor Siglec-8 in chronic rhinosinusitis with hyperplastic nasal polyposis., PMID:33755113
First Evidence for a Role of Siglec-8 in Breast Cancer., PMID:33670444
Discovery, Function, and Therapeutic Targeting of Siglec-8., PMID:33374255
Mechanisms and biomarkers of inflammatory endotypes in chronic rhinosinusitis without nasal polyps., PMID:33326802
Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration., PMID:33289969
Anti-Siglec-8 Antibody for Eosinophilic Gastritis and Duodenitis., PMID:33085861
Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins., PMID:32915505
A monoclonal antibody to Siglec-8 suppresses non-allergic airway inflammation and inhibits IgE-independent mast cell activation., PMID:32814824
Isolation, identification, and characterization of the human airway ligand for the eosinophil and mast cell immunoinhibitory receptor Siglec-8., PMID:32791164
A FACS-based approach to obtain viable eosinophils from human adipose tissue., PMID:32764552
A Potent Mimetic of the Siglec-8 Ligand 6'-Sulfo-Sialyl Lewisx., PMID:32744401
For research use only. Not for human or drug use.
