Catalog No.
AHN57201
Biological activity
Measured by its ability to transfer sulfate from PAPS to N-acetyl-D-glucosamine. The specific activity is >150 pmol/min/μg, as measured under the described conditions.
Expression system
Mammalian Cells
Species
Homo sapiens (Human)
Protein length
His29-His386
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
Q8NCG5
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
GlcNAc6ST-2, LSST, HEC-GlcNAc6ST, GST-3, N-acetylglucosamine 6-O-sulfotransferase 2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3, CHST4, Carbohydrate sulfotransferase 4, L-selectin ligand sulfotransferase, Gn6st-2, High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
Molecular Mechanisms of Tumor Progression and Novel Therapeutic and Diagnostic Strategies in Mesothelioma., PMID:40362535
CHST4 associates with high-abundance immune infiltration in hormone receptor-positive breast cancer., PMID:39213175
GlcNAc6ST2/CHST4 Is Essential for the Synthesis of R-10G-Reactive Keratan Sulfate/Sulfated N-Acetyllactosamine Oligosaccharides in Mouse Pleural Mesothelium., PMID:38398516
CHST4 Gene as a Potential Predictor of Clinical Outcome in Malignant Pleural Mesothelioma., PMID:38396947
N-acetylglucosamine-6-O sulfation on intestinal mucins prevents obesity and intestinal inflammation by regulating gut microbiota., PMID:37463055
Prognostic value and immunological role of BAIAP2L2 in liver hepatocellular carcinoma: A pan-cancer analysis., PMID:36338652
Tumor necrosis factor receptor regulation of peripheral node addressin biosynthetic components in tumor endothelial cells., PMID:36189308
Identification of N-Glycoproteins of Knee Cartilage from Adult Osteoarthritis and Kashin-Beck Disease Based on Quantitative Glycoproteomics, Compared with Normal Control Cartilage., PMID:36010590
CHST4 might promote the malignancy of cholangiocarcinoma., PMID:35294478
Endogenous hydrogen sulfide promotes human preimplantation embryonic development by regulating metabolism-related gene expression., PMID:34995767
Identification of key genes and pathways in mild and severe nonalcoholic fatty liver disease by integrative analysis., PMID:34786546
Exploring the sulfate patterns of chondroitin sulfate/dermatan sulfate and keratan sulfate in human pancreatic cancer., PMID:34464868
Analysis of A4gnt Knockout Mice Reveals an Essential Role for Gastric Sulfomucins in Preventing Gastritis Cystica Profunda., PMID:31246144
Single-Cell Analysis Reveals Heterogeneity of High Endothelial Venules and Different Regulation of Genes Controlling Lymphocyte Entry to Lymph Nodes., PMID:30865898
Distinct substrate specificities of human GlcNAc-6-sulfotransferases revealed by mass spectrometry-based sulfoglycomic analysis., PMID:30093410
Tumor-dependent Effects of Proteoglycans and Various Glycosaminoglycan Synthesizing Enzymes and Sulfotransferases on Patients' Outcome., PMID:29984655
Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle., PMID:29362381
Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases., PMID:21169395
A versatile polyacrylamide gel electrophoresis based sulfotransferase assay., PMID:20146816
IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewisx epitopes in the human bronchial mucosa., PMID:17944600
Transgenic LacZ under control of Hec-6st regulatory sequences recapitulates endogenous gene expression on high endothelial venules., PMID:17360566
Assignment of BGLAP, BMP2, CHST4, SLC1A3, SLC4A1, SLC9A5 and SLC20A1 to equine chromosomes by FISH and confirmation by RH mapping., PMID:16167999
A HEV-restricted sulfotransferase is expressed in rheumatoid arthritis synovium and is induced by lymphotoxin-alpha/beta and TNF-alpha in cultured endothelial cells., PMID:15752429
Effects of NO-1886 (Ibrolipim), a lipoprotein lipase-promoting agent, on gene induction of cytochrome P450s, carboxylesterases, and sulfotransferases in primary cultures of human hepatocytes., PMID:15681896
CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin., PMID:11310842
For research use only. Not for human or drug use.
