Catalog No.
YHB40801
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Gln362
Predicted molecular weight
44.27 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
B4DZS0
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Caspase-activated nuclease, DFF2, Caspase-activated DNase, CAD, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, DFF40, DFFB, DFF-40, CPAN
Analysis of a cell-free DNA-based cancer screening cohort links fragmentomic profiles, nuclease levels, and plasma DNA concentrations., PMID:39603706
DNA fragmentation factor 40-based therapeutic approaches for cancer: a review article., PMID:39397131
GWAS shows the genetics behind cell-free DNA and highlights the importance of p.Arg206Cys in DNASE1L3 for non-invasive testing., PMID:39331505
The Caspase-Activated DNase drives inflammation and contributes to defense against viral infection., PMID:38849575
Genomic origin, fragmentomics, and transcriptional properties of long cell-free DNA molecules in human plasma., PMID:38408788
MEG3 Regulates CSE-Induced Apoptosis by Regulating miR-421/DFFB Signal Axis., PMID:37215747
Fragmentation landscape of cell-free DNA revealed by deconvolutional analysis of end motifs., PMID:37075072
Multi-CpG linear regression models to accurately predict paclitaxel and docetaxel activity in cancer cell lines., PMID:36990534
Jagged Ends on Multinucleosomal Cell-Free DNA Serve as a Biomarker for Nuclease Activity and Systemic Lupus Erythematosus., PMID:35587043
DFF40 deficiency in cancerous T cells is implicated in chemotherapy drug sensitivity and resistance through the regulation of the apoptotic pathway., PMID:34678222
The Nexus of cfDNA and Nuclease Biology., PMID:34006390
Survivin Promoter-Driven DFF40 Gene Expression Sensitizes Melanoma Cancer Cells to Chemotherapy., PMID:33960240
Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability., PMID:33465003
The role of the DFF40/CAD endonuclease in genomic stability., PMID:33387146
Design and characterization of a recombinant immunotoxin for targeted therapy of breast cancer cells: In vitro and in silico analyses., PMID:33301810
HNRNPCL1, PRAMEF1, CFAP74, and DFFB: Common Potential Biomarkers for Sporadic and Suspected Lynch Syndrome Endometrial Cancer., PMID:33177874
The Biology of Cell-free DNA Fragmentation and the Roles of DNASE1, DNASE1L3, and DFFB., PMID:32004449
DNA fragmentation factor 40 expression in T cells confers sensibility to tributyltin-induced apoptosis., PMID:31401084
Proteasome inhibitors trigger mutations via activation of caspases and CAD, but mutagenesis provoked by the HDAC inhibitors vorinostat and romidepsin is caspase/CAD-independent., PMID:30997620
Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes., PMID:30799275
Cell-Free DNA Release by Mouse Fetal Membranes., PMID:30572800
Non-endometrioid and high-grade endometrioid endometrial cancers show DNA fragmentation factor 40 (DFF40) and B-cell lymphoma 2 protein (BCL2) underexpression, which predicts disease-free and overall survival, but not DNA fragmentation factor 45 (DFF45) underexpression., PMID:29653556
Exome Sequencing Identifies Genetic Variants Associated with Circulating Lipid Levels in Mexican Americans: The Insulin Resistance Atherosclerosis Family Study (IRASFS)., PMID:29618726
The tumor antagonistic steroidal alkaloid Solanidine prompts the intrinsic suicidal signal mediated DFF-40 nuclear import and nucleosomal disruption., PMID:29524520
Endometrial Polyps and Benign Endometrial Hyperplasia Have Increased Prevalence of DNA Fragmentation Factors 40 and 45 (DFF40 and DFF45) Together With the Antiapoptotic B-Cell Lymphoma (Bcl-2) Protein Compared With Normal Human Endometria., PMID:28914671
CIDE domains form functionally important higher-order assemblies for DNA fragmentation., PMID:28652364
Loss of Caspase-Activated DNase Protects Against Atherosclerosis in Apolipoprotein E-Deficient Mice., PMID:28007744
DNase γ, DNase I and caspase-activated DNase cooperate to degrade dead cells., PMID:27654959
Upregulated expression of BCL2, MCM7, and CCNE1 indicate cisplatin-resistance in the set of two human bladder cancer cell lines: T24 cisplatin sensitive and T24R2 cisplatin resistant bladder cancer cell lines., PMID:26966728
An intrinsic DFF40/CAD endonuclease deficiency impairs oligonucleosomal DNA hydrolysis during caspase-dependent cell death: a common trait in human glioblastoma cells., PMID:26755073
Sensitization of breast cancer cells to doxorubicin via stable cell line generation and overexpression of DFF40., PMID:26529233
Data for a proteomic analysis of p53-independent induction of apoptosis by bortezomib., PMID:26217687
The effects of grape seed on apoptosis-related gene expression and oxidative stress in streptozotocin-induced diabetic rats., PMID:25565258
Dietary restriction preserves the mass and function of pancreatic β cells via cell kinetic regulation and suppression of oxidative/ER stress in diabetic mice., PMID:25488546
A proteomic analysis of p53-independent induction of apoptosis by bortezomib in 4T1 breast cancer cell line., PMID:25305590
Stable overexpression of DNA fragmentation factor in T-47D cells: sensitization of breast cancer cells to apoptosis in response to acetazolamide and sulfabenzamide., PMID:25086620
Safeguarding Stem Cell-Based Regenerative Therapy against Iatrogenic Cancerogenesis: Transgenic Expression of DNASE1, DNASE1L3, DNASE2, DFFB Controlled By POLA1 Promoter in Proliferating and Directed Differentiation Resisting Human Autologous Pluripotent Induced Stem Cells Leads to their Death., PMID:25045589
DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis., PMID:24312463
GM-CSF-DFF40: a novel humanized immunotoxin induces apoptosis in acute myeloid leukemia cells., PMID:23529188
Chromatin collapse during caspase-dependent apoptotic cell death requires DNA fragmentation factor, 40-kDa subunit-/caspase-activated deoxyribonuclease-mediated 3'-OH single-strand DNA breaks., PMID:23430749
[Study on gene differential expressions of substance and energy metabolism in chronic superficial gastritis patients of Pi deficiency syndrome and of pi-wei hygropyrexia syndrome]., PMID:23185754
[Repressional effects of the glutamate antibodies on expression of Dffb gene in the brain of rats with experimental Alzheimer's disease]., PMID:23156675
Sevoflurane and isoflurane preconditioning provides neuroprotection by inhibition of apoptosis-related mRNA expression in a rat model of focal cerebral ischemia., PMID:22871953
Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40., PMID:22609799
Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease., PMID:22253444
HepG2 cells infected with Klebsiella pneumoniae show DNA laddering at apoptotic and necrotic stages., PMID:22038342
Early apoptotic reorganization of spliceosomal proteins involves caspases, CAD and rearrangement of NuMA., PMID:22023725
Nonsynonymous single-nucleotide polymorphisms of the human apoptosis-related endonuclease--DNA fragmentation factor beta polypeptide, endonuclease G, and Flap endonuclease-1--genes show a low degree of genetic heterogeneity., PMID:22011247
Cheating death at the dawn of life: developmental control of apoptotic repression in the preimplantation embryo., PMID:21888895
Septic sera induces apoptosis and DNA fragmentation factor 40 activation in fibroblasts., PMID:21820410
For research use only. Not for human or drug use.
