Anti-Semaglutide ELISA Kit (KAK13901)

Catalog No.

KAK13901

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Semaglutide has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-Semaglutide present is bound by the immobilized protein. After washing away any unbound substances, a HRP conjugated probe is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Semaglutide bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Anti-Semaglutide concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

15.63 - 1,000 ng/mL

Sensitivity

6.49 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	519.9	135.2	30.9	542.8	112.8	17.3
Standard deviation	28.3	9.5	2.0	71.5	16.7	2.5
CV (%)	5.5	7.1	6.4	13.2	14.8	14.7

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.

Alternative Names

CAS, 910463-68-2, Semaglutide, Rybelsus, Ozempic, NN9535, OG217SC, NNC 0113-0217

Document Download

Anti-Semaglutide ELISA Kit.pdf