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Rabies virus (RABV) Matrix protein (M) ELISA Kit

Catalog #:   KVV37701 Specific References (50) DATASHEET
Applications: Used for the quantitative determination of Rabies virus (RABV) Matrix protein (M) concentration in serum and plasma.
Sample type: Plasma, Serum
Sensitivity: 0.59 ng/mL
Range: 2.34 - 150 ng/mL
Overview

Catalog No.

KVV37701

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Rabies virus (RABV) Matrix protein (M) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Rabies virus (RABV) Matrix protein (M) present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Rabies virus (RABV) Matrix protein (M) is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Rabies virus (RABV) Matrix protein (M) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Specificity

Rabies virus (RABV) Matrix protein (M)

Applications

Used for the quantitative determination of Rabies virus (RABV) Matrix protein (M) concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

2.34 - 150 ng/mL

Sensitivity

0.59 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

 

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

16

16

16

24

24

24

Mean (ng/mL)

74.8

19.7

4.8

75.9

20.2

4.9

Standard deviation

3.8

0.8

0.2

4.2

0.8

0.3

CV (%)

5.1

4.0

4.5

5.5

3.9

5.9

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Data Image
  • Experiment Example
    CALCULATION OF RESULTS
    Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Rabies virus (RABV) Matrix protein (M) concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
References

Development of an oral gut-targeted rabies virus-like particles (RVLPs) vaccine with mucosal immune adjuvant LTB via delivering of localized-release microparticles., PMID:40476513

Dynamic bidirectional regulation between Stk38 and rabies virus M protein coordinates apoptosis progression during neurotropic infection., PMID:40398756

The rabies virus matrix protein (RABV M) interacts with host histone deacetylase 6 (HDAC6) to activate the MEK/ ERK signaling pathway and enhance viral replication., PMID:40300412

Structural Heterogeneity of the Rabies Virus Virion., PMID:39339924

The roles of rabies virus structural proteins in immune evasion and implications for vaccine development., PMID:39297428

A nucleoside-modified mRNA vaccine forming rabies virus-like particle elicits strong cellular and humoral immune responses against rabies virus infection in mice., PMID:39129566

Single Amino Acid Substitution in the Matrix Protein of Rabies Virus Is Associated with Neurovirulence in Mice., PMID:38793581

The matrix protein of lyssavirus hijacks autophagosome for efficient egress by recruiting NEDD4 through its PPxY motif., PMID:38566321

TRIM72 restricts lyssavirus infection by inducing K48-linked ubiquitination and proteasome degradation of the matrix protein., PMID:38408103

Fusion of Rabies Virus Glycoprotein or gh625 to Iduronate-2-Sulfatase for the Treatment of Mucopolysaccharidosis Type II., PMID:37212263

A novel epitope tag from rabies virus has versatile in vitro applications., PMID:37166480

Sf9 Cell Metabolism Throughout the Recombinant Baculovirus and Rabies Virus-Like Particles Production in Two Culture Systems., PMID:37162721

Morphogenesis of Bullet-Shaped Rabies Virus Particles Regulated by TSG101., PMID:37042780

Host Desmin Interacts with RABV Matrix Protein and Facilitates Virus Propagation., PMID:36851648

Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses., PMID:36680128

Proteomic analysis of canine vaccines., PMID:36662608

Biochemical monitoring throughout all stages of rabies virus-like particles production by Raman spectroscopy using global models., PMID:36587847

Henipaviruses and lyssaviruses target nucleolar treacle protein and regulate ribosomal RNA synthesis., PMID:36479968

Sf9 Cells Metabolism and Viability When Coinfected with Two Monocistronic Baculoviruses to Produce Rabies Virus-like Particles., PMID:36396754

Looking at the Pathogenesis of the Rabies Lyssavirus Strain Pasteur Vaccins through a Prism of the Disorder-Based Bioinformatics., PMID:36291645

Atomic model of vesicular stomatitis virus and mechanism of assembly., PMID:36216930

The Amino Acid at Position 95 in the Matrix Protein of Rabies Virus Is Involved in Antiviral Stress Granule Formation in Infected Cells., PMID:36069552

Lab-Attenuated Rabies Virus Facilitates Opening of the Blood-Brain Barrier by Inducing Matrix Metallopeptidase 8., PMID:36005758

Visualizing molecular interactions that determine assembly of a bullet-shaped vesicular stomatitis virus particle., PMID:35970826

Enteric viruses replicate in salivary glands and infect through saliva., PMID:35768512

Rabies Virus Exploits Cytoskeleton Network to Cause Early Disease Progression and Cellular Dysfunction., PMID:35685339

Early Transcriptional Changes in Rabies Virus-Infected Neurons and Their Impact on Neuronal Functions., PMID:34970230

Production of Rabies VLPs in Insect Cells by Two Monocistronic Baculoviruses Approach., PMID:34228257

Efficacy of a low-dose candidate malaria vaccine, R21 in adjuvant Matrix-M, with seasonal administration to children in Burkina Faso: a randomised controlled trial., PMID:33964223

Analyses of cell death mechanisms related to amino acid substitution at position 95 in the rabies virus matrix protein., PMID:33891533

Rabies virus matrix protein targets host actin cytoskeleton: a protein-protein interaction analysis., PMID:33289839

The ATPase ATP6V1A facilitates rabies virus replication by promoting virion uncoating and interacting with the viral matrix protein., PMID:33208464

Components and Architecture of the Rhabdovirus Ribonucleoprotein Complex., PMID:32872471

Characterization of the Immune Response of MERS-CoV Vaccine Candidates Derived from Two Different Vectors in Mice., PMID:31968702

The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication., PMID:31861477

Lyssavirus matrix protein cooperates with phosphoprotein to modulate the Jak-Stat pathway., PMID:31434934

Generation of Monoclonal Antibodies against Variable Epitopes of the M Protein of Rabies Virus., PMID:31018607

Molecular Fingerprinting of On-Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits., PMID:30377226

Segmentation of the rabies virus genome., PMID:29787783

Regulation of NF-κB by the p105-ABIN2-TPL2 complex and RelAp43 during rabies virus infection., PMID:29084252

Phosphoprotein Gene Contributes to the Enhanced Apoptosis Induced by Wild-Type Rabies Virus GD-SH-01 In Vitro., PMID:28928726

Pathogenicity and Immunogenicity of Recombinant Rabies Viruses Expressing the Lagos Bat Virus Matrix and Glycoprotein: Perspectives for a Pan-Lyssavirus Vaccine., PMID:30270894

Increased transgene expression level of rabies virus vector for transsynaptic tracing., PMID:28700657

Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6., PMID:28491824

The matrix protein of rabies virus binds to RelAp43 to modulate NF-κB-dependent gene expression related to innate immunity., PMID:28000711

Roles of nuclear trafficking in infection by cytoplasmic negative-strand RNA viruses: paramyxoviruses and beyond., PMID:27498841

Wild-type rabies virus induces autophagy in human and mouse neuroblastoma cell lines., PMID:27463027

Rabies virus matrix protein induces apoptosis by targeting mitochondria., PMID:27426727

Rabies virus inactivates cofilin to facilitate viral budding and release., PMID:27396619

[Expression and Purification of M Protein of RV in Baculovirus and Preparation of Its Polyclonal Antibody]., PMID:29995370

Datasheet
$ 1128
Product specifications
96 T 1128

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Rabies virus (RABV) Matrix protein (M) ELISA Kit [KVV37701]
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