Catalog No.
KVV37701
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Rabies virus (RABV) Matrix protein (M) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Rabies virus (RABV) Matrix protein (M) present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Rabies virus (RABV) Matrix protein (M) is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Rabies virus (RABV) Matrix protein (M) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Rabies virus (RABV) Matrix protein (M) concentration in serum and plasma.
Specificity
Rabies virus (RABV) Matrix protein (M)
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
2.34 - 150 ng/mL
Sensitivity
0.59 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (ng/mL)
|
74.8
|
19.7
|
4.8
|
75.9
|
20.2
|
4.9
|
|
Standard deviation
|
3.8
|
0.8
|
0.2
|
4.2
|
0.8
|
0.3
|
|
CV (%)
|
5.1
|
4.0
|
4.5
|
5.5
|
3.9
|
5.9
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Note
For Research Use Only.
[Expression and Purification of M Protein of RV in Baculovirus and Preparation of Its Polyclonal Antibody]., PMID:29995370
Rabies virus inactivates cofilin to facilitate viral budding and release., PMID:27396619
Rabies virus matrix protein induces apoptosis by targeting mitochondria., PMID:27426727
Wild-type rabies virus induces autophagy in human and mouse neuroblastoma cell lines., PMID:27463027
Roles of nuclear trafficking in infection by cytoplasmic negative-strand RNA viruses: paramyxoviruses and beyond., PMID:27498841
The matrix protein of rabies virus binds to RelAp43 to modulate NF-κB-dependent gene expression related to innate immunity., PMID:28000711
Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6., PMID:28491824
Increased transgene expression level of rabies virus vector for transsynaptic tracing., PMID:28700657
Pathogenicity and Immunogenicity of Recombinant Rabies Viruses Expressing the Lagos Bat Virus Matrix and Glycoprotein: Perspectives for a Pan-Lyssavirus Vaccine., PMID:30270894
Phosphoprotein Gene Contributes to the Enhanced Apoptosis Induced by Wild-Type Rabies Virus GD-SH-01 In Vitro., PMID:28928726
Regulation of NF-κB by the p105-ABIN2-TPL2 complex and RelAp43 during rabies virus infection., PMID:29084252
Segmentation of the rabies virus genome., PMID:29787783
Molecular Fingerprinting of On-Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits., PMID:30377226
Generation of Monoclonal Antibodies against Variable Epitopes of the M Protein of Rabies Virus., PMID:31018607
Lyssavirus matrix protein cooperates with phosphoprotein to modulate the Jak-Stat pathway., PMID:31434934
The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication., PMID:31861477
Characterization of the Immune Response of MERS-CoV Vaccine Candidates Derived from Two Different Vectors in Mice., PMID:31968702
Components and Architecture of the Rhabdovirus Ribonucleoprotein Complex., PMID:32872471
The ATPase ATP6V1A facilitates rabies virus replication by promoting virion uncoating and interacting with the viral matrix protein., PMID:33208464
Rabies virus matrix protein targets host actin cytoskeleton: a protein-protein interaction analysis., PMID:33289839
Analyses of cell death mechanisms related to amino acid substitution at position 95 in the rabies virus matrix protein., PMID:33891533
Efficacy of a low-dose candidate malaria vaccine, R21 in adjuvant Matrix-M, with seasonal administration to children in Burkina Faso: a randomised controlled trial., PMID:33964223
Production of Rabies VLPs in Insect Cells by Two Monocistronic Baculoviruses Approach., PMID:34228257
Early Transcriptional Changes in Rabies Virus-Infected Neurons and Their Impact on Neuronal Functions., PMID:34970230
Rabies Virus Exploits Cytoskeleton Network to Cause Early Disease Progression and Cellular Dysfunction., PMID:35685339
Enteric viruses replicate in salivary glands and infect through saliva., PMID:35768512
Visualizing molecular interactions that determine assembly of a bullet-shaped vesicular stomatitis virus particle., PMID:35970826
Lab-Attenuated Rabies Virus Facilitates Opening of the Blood-Brain Barrier by Inducing Matrix Metallopeptidase 8., PMID:36005758
The Amino Acid at Position 95 in the Matrix Protein of Rabies Virus Is Involved in Antiviral Stress Granule Formation in Infected Cells., PMID:36069552
Atomic model of vesicular stomatitis virus and mechanism of assembly., PMID:36216930
Looking at the Pathogenesis of the Rabies Lyssavirus Strain Pasteur Vaccins through a Prism of the Disorder-Based Bioinformatics., PMID:36291645
Sf9 Cells Metabolism and Viability When Coinfected with Two Monocistronic Baculoviruses to Produce Rabies Virus-like Particles., PMID:36396754
Henipaviruses and lyssaviruses target nucleolar treacle protein and regulate ribosomal RNA synthesis., PMID:36479968
Biochemical monitoring throughout all stages of rabies virus-like particles production by Raman spectroscopy using global models., PMID:36587847
Proteomic analysis of canine vaccines., PMID:36662608
Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses., PMID:36680128
Host Desmin Interacts with RABV Matrix Protein and Facilitates Virus Propagation., PMID:36851648
Morphogenesis of Bullet-Shaped Rabies Virus Particles Regulated by TSG101., PMID:37042780
Sf9 Cell Metabolism Throughout the Recombinant Baculovirus and Rabies Virus-Like Particles Production in Two Culture Systems., PMID:37162721
A novel epitope tag from rabies virus has versatile in vitro applications., PMID:37166480
Fusion of Rabies Virus Glycoprotein or gh625 to Iduronate-2-Sulfatase for the Treatment of Mucopolysaccharidosis Type II., PMID:37212263
TRIM72 restricts lyssavirus infection by inducing K48-linked ubiquitination and proteasome degradation of the matrix protein., PMID:38408103
The matrix protein of lyssavirus hijacks autophagosome for efficient egress by recruiting NEDD4 through its PPxY motif., PMID:38566321
Single Amino Acid Substitution in the Matrix Protein of Rabies Virus Is Associated with Neurovirulence in Mice., PMID:38793581
A nucleoside-modified mRNA vaccine forming rabies virus-like particle elicits strong cellular and humoral immune responses against rabies virus infection in mice., PMID:39129566
The roles of rabies virus structural proteins in immune evasion and implications for vaccine development., PMID:39297428
Structural Heterogeneity of the Rabies Virus Virion., PMID:39339924
The rabies virus matrix protein (RABV M) interacts with host histone deacetylase 6 (HDAC6) to activate the MEK/ ERK signaling pathway and enhance viral replication., PMID:40300412
Dynamic bidirectional regulation between Stk38 and rabies virus M protein coordinates apoptosis progression during neurotropic infection., PMID:40398756
Development of an oral gut-targeted rabies virus-like particles (RVLPs) vaccine with mucosal immune adjuvant LTB via delivering of localized-release microparticles., PMID:40476513
For research use only. Not for human or drug use.
