SARS-CoV-2 RBD (JN.1) ELISA Kit

Catalog No.

KVV00304

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for SARS-CoV-2 RBD (JN.1) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any SARS-CoV-2 RBD (JN.1) present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for SARS-CoV-2 RBD (JN.1) is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 RBD (JN.1) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Specificity

SARS-CoV-2 RBD (JN.1)

Applications

Used for the quantitative determination of SARS-CoV-2 RBD (JN.1) concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

12.5 - 800 pg/mL

Sensitivity

8.26 pg/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/mL)	398.4	98.9	19.4	402.0	94.9	20.2
Standard deviation	11.5	2.8	1.3	22.7	3.1	1.7
CV (%)	2.9	2.8	6.6	5.6	3.3	8.3

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.

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