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Catalog No.
KVV00304
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for SARS-CoV-2 RBD (JN.1) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any SARS-CoV-2 RBD (JN.1) present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for SARS-CoV-2 RBD (JN.1) is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 RBD (JN.1) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
SARS-CoV-2 RBD (JN.1)
Applications
Used for the quantitative determination of SARS-CoV-2 RBD (JN.1) concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
12.5 - 800 pg/mL
Sensitivity
8.26 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (pg/mL) |
398.4 |
98.9 |
19.4 |
402.0 |
94.9 |
20.2 |
|
Standard deviation |
11.5 |
2.8 |
1.3 |
22.7 |
3.1 |
1.7 |
|
CV (%) |
2.9 |
2.8 |
6.6 |
5.6 |
3.3 |
8.3 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.
For research use only. Not for human or drug use.
