Catalog No.
PVV01201
Species reactivity
SARS-CoV-2 (2019-nCoV)
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant SARS-CoV-2 NSP3/PL-PRO (Glu1024-Gln1198).
Tested applications
ELISA: 1:4000-1:8000, WB: 1:2000-1:8000
Target
Non-structural protein 3,nsp3,PL2-PRO,Papain-like protease,Papain-like proteinase,PL-PRO,Replicase polyprotein 1ab,pp1ab,ORF1ab polyprotein
Purification
Purified by antigen affinity column.
Accession
P0DTD1
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Investigation of Immunoreactivity Profiles and Epitope Landscape in Divergent COVID-19 Trajectories and SARS-CoV-2 Variants., PMID:39873496
Proteome-Wide Analysis of Antibody Responses in Asymptomatic Omicron BA.2-Infected Individuals at the Amino Acid Resolution., PMID:39661118
MDA5 Is a Major Determinant of Developing Symptoms in Critically Ill COVID-19 Patients., PMID:39460899
SARS-CoV-2 Genotyping Highlights the Challenges in Spike Protein Drift Independent of Other Essential Proteins., PMID:39338537
The evolutionary and molecular history of a chikungunya virus outbreak lineage., PMID:39058744
How does a Saccharomyces cerevisiae extract influence the components of isolated rotavirus particles from stool samples collected in a clinical setting from children?, PMID:38946847
Identification of Three Novel Linear B-Cell Epitopes in Non-Structural Protein 3 of Porcine Epidemic Diarrhea Virus Using Monoclonal Antibodies., PMID:38543789
Visualization of Early RNA Replication Kinetics of SARS-CoV-2 by Using Single Molecule RNA-FISH Combined with Immunofluorescence., PMID:38400039
Antibodies against SARS-CoV-2 non-structural protein 3 cross-react with human muscle cells and neuroglial cells., PMID:38281898
A Novel Rotavirus Reverse Genetics Platform Supports Flexible Insertion of Exogenous Genes and Enables Rapid Development of a High-Throughput Neutralization Assay., PMID:37896813
Recombinant rotavirus expressing the glycosylated S1 protein of SARS-CoV-2., PMID:37830788
Mucosal and systemic neutralizing antibodies to norovirus induced in infant mice orally inoculated with recombinant rotaviruses., PMID:36821582
Computational design of candidate multi-epitope vaccine against SARS-CoV-2 targeting structural (S and N) and non-structural (NSP3 and NSP12) proteins., PMID:36744449
Targeting viral proteins for restraining SARS-CoV-2: focusing lens on viral proteins beyond spike for discovering new drug targets., PMID:36723288
The Cold-Adapted, Temperature-Sensitive SARS-CoV-2 Strain TS11 Is Attenuated in Syrian Hamsters and a Candidate Attenuated Vaccine., PMID:36680135
Novel neutralizing chicken IgY antibodies targeting 17 potent conserved peptides identified by SARS-CoV-2 proteome microarray, and future prospects., PMID:36618358
Generation of Recombinant Rotaviruses Expressing Human Norovirus Capsid Proteins., PMID:36314817
A Chikungunya Virus Multiepitope Recombinant Protein Expressed from the Binary System Insect Cell/Recombinant Baculovirus Is Useful for Laboratorial Diagnosis of Chikungunya., PMID:35889170
Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes., PMID:35758692
Antibodies targeting conserved non-canonical antigens and endemic coronaviruses associate with favorable outcomes in severe COVID-19., PMID:35738278
Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells., PMID:35730969
A Detailed Overview of Immune Escape, Antibody Escape, Partial Vaccine Escape of SARS-CoV-2 and Their Emerging Variants With Escape Mutations., PMID:35222380
RNAi-mediated siRNA sequences to combat the COVID-19 pandemic with the inhibition of SARS-CoV2., PMID:35071824
SARS-CoV-2 immune repertoire in MIS-C and pediatric COVID-19., PMID:34611361
Dynamic landscape mapping of humoral immunity to SARS-CoV-2 identifies non-structural protein antibodies associated with the survival of critical COVID-19 patients., PMID:34404759
Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies., PMID:34270554
Molecular insight into the genomic variation of SARS-CoV-2 strains from current outbreak., PMID:34166886
Potential transmission chains of variant B.1.1.7 and co-mutations of SARS-CoV-2., PMID:34127650
SARS-CoV-2 Proteins: Are They Useful as Targets for COVID-19 Drugs and Vaccines?, PMID:33622224
Distinct antibody repertoires against endemic human coronaviruses in children and adults., PMID:33497357
Infectious RNA vaccine protects mice against chikungunya virus infection., PMID:33273501
The Inhaled Steroid Ciclesonide Blocks SARS-CoV-2 RNA Replication by Targeting the Viral Replication-Transcription Complex in Cultured Cells., PMID:33055254
Non-synonymous mutations of SARS-CoV-2 leads epitope loss and segregates its variants., PMID:33049387
The use of knowledge management tools in viroinformatics. Example study of a highly conserved sequence motif in Nsp3 of SARS-CoV-2 as a therapeutic target., PMID:32828990
Hypervariable Domain of nsP3 of Eastern Equine Encephalitis Virus Is a Critical Determinant of Viral Virulence., PMID:32581106
Comparative Sequence Analysis of Historic and Current Porcine Rotavirus C Strains and Their Pathogenesis in 3-Day-Old and 3-Week-Old Piglets., PMID:32395116
Visualization of cell-type dependent effects of anti-E2 antibody and interferon-gamma treatments on localization and expression of Broccoli aptamer-tagged alphavirus RNAs., PMID:32210257
Both ADP-Ribosyl-Binding and Hydrolase Activities of the Alphavirus nsP3 Macrodomain Affect Neurovirulence in Mice., PMID:32047134
Specific recombinant proteins of porcine epidemic diarrhea virus are immunogenic, revealing their potential use as diagnostic markers., PMID:31500721
Differential recognition of peptides within feline coronavirus polyprotein 1 ab by sera from healthy cats and cats with feline infectious peritonitis., PMID:31048107
Rotavirus shedding following administration of RV3-BB human neonatal rotavirus vaccine., PMID:28481726
Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of chikungunya virus: potential use in basic research., PMID:26280524
Stress granule components G3BP1 and G3BP2 play a proviral role early in Chikungunya virus replication., PMID:25653451
The SARS-coronavirus papain-like protease: structure, function and inhibition by designed antiviral compounds., PMID:25554382
Unique epitopes recognized by antibodies induced in Chikungunya virus-infected non-human primates: implications for the study of immunopathology and vaccine development., PMID:24755730
Isolation, propagation, genome analysis and epidemiology of HKU1 betacoronaviruses., PMID:24394697
Novel attenuated Chikungunya vaccine candidates elicit protective immunity in C57BL/6 mice., PMID:24371047
Hypervariable domain of nonstructural protein nsP3 of Venezuelan equine encephalitis virus determines cell-specific mode of virus replication., PMID:23637407
Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development., PMID:23015702
Repeated circulation over 6 years of intergenogroup mono-reassortant G2P[4] rotavirus strains with genotype N1 of the NSP2 gene., PMID:22575821
For research use only. Not for human or drug use.
